Difference between revisions of "Team:FAFU-CHINA/Basic Part"

Line 1: Line 1:
 +
{{:Team:FAFU-CHINA/menu2}}
 +
<html>
 +
  <div id="main-content" class="container">
 +
    <div class="col-md-3" id="document-sidebar">
 +
</html>
 +
__TOC__
 +
__NOEDITSECTION__
 +
<html>
 +
    </div>
 +
  <script>
 +
        $("#toctitle").remove();
 +
var toc_tbl = $("#toc")
 +
    .prop("id","table-contents")
 +
    .addClass("panel panel-primary")
 +
    .removeClass("toc")
 +
    .children()
 +
      .clone();
 +
       
 +
       
 +
$("#document-sidebar")
 +
.children()
 +
.remove()
 +
.end()
 +
.html('<div class="bs-docs-sidebar hidden-print hidden-xs hidden-sm" id="page-contents"></div>');
 +
 +
toc_tbl.appendTo("#page-contents");
 +
        $("#page-contents ul").addClass("nav");
 +
        $("#page-contents > ul").addClass("bs-docs-sidenav");
 +
        $("#toctitle").remove();
 +
        $(".tocnumber").remove();
 +
  </script>
 +
    <div class="col-md-9" id="document-body">
 +
</html>
 +
 +
    <!-- theme -->
 +
<html>
 +
<head>
 +
  <meta charset="utf-8">
 +
  <meta http-equiv="X-UA-Compatible" content="IE=edge">
 +
  <meta name="viewport" content="width=device-width, initial-scale=1,maximum-scale=1.0,user-scalable=no">
 +
  <meta name="description" content="">
 +
  <meta name="author" content="">
 +
  <meta http-equiv="X-UA-Compatible" content="IE=edge" />
 +
  <link rel="shortcut icon" href="/favicon.ico">
 +
 +
  <title>Team FAFU-CHINA</title>
 +
 +
  <!-- Bootstrap core CSS -->
 +
 +
  <link rel="stylesheet" href="https://2016.igem.org/Team:FAFU-CHINA/page/css/toolbar?action=raw&ctype=text/css">
 +
  <link rel="stylesheet" href="https://2016.igem.org/Team:FAFU-CHINA/page/css/cstyle?action=raw&ctype=text/css">
 +
 +
 +
 +
  <style>
 +
  body {
 +
  font-family: Lato, inherit;
 +
}
 +
.bs-docs-header #title-icon{
 +
  margin: auto;
 +
  display: block;
 +
}
 +
.team-content h2{
 +
    font-size: 1em;
 +
}
 +
.team-content .team-member h2{
 +
    text-align: center;
 +
}
 +
.team-content h3{
 +
    font-size: 0.8em;
 +
}
 +
.team-content .team-member h3{
 +
    text-align: center;
 +
}
 +
.team-content p{
 +
    margin-left: 15px;
 +
    margin-right: 15px;
 +
    font-size: 0.8em;
 +
}
 +
 +
.team-content .team-member p{
 +
    text-align: center;
 +
    height: 100px;
 +
    overflow: auto;
 +
}
 +
.team-content .team-member img{
 +
    width: 100%;
 +
    max-width: 300px;
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
    display: block;
 +
    max-height: 100%;
 +
    margin-top: 25px;
 +
    margin-bottom: 25px;
 +
}
 +
.team-content .group-photo img{
 +
    width: 100%;
 +
    max-width: 1000px;
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
    display: block;
 +
    max-height: 100%;
 +
    margin-top: 25px;
 +
    margin-bottom: 25px;
 +
}
 +
.svg {
 +
    width:100%;
 +
    height:300px;
 +
}
 +
  #content {
 +
    margin-top: 70px;
 +
    font-family: Lato, inherit;
 +
  }
 +
.footer img {
 +
    width: 100%;
 +
    max-width: 200px;
 +
    display: block;
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
}
 +
  </style>
 +
 +
 +
 
 +
 +
 +
  <!-- HTML5 shim and Respond.js IE8 support of HTML5 elements and media queries -->
 +
  <!--[if lt IE 9]>
 +
      <script src="https://2016.igem.org/Team:FAFU-CHINA/page/css/html5shiv?action=raw&ctype=text/javascrip"></script>
 +
      <script src="https://2016.igem.org/Team:FAFU-CHINA/page/css/respond?action=raw&ctype=text/javascrip"></script>
 +
 +
    <![endif]-->
 +
 +
</head>
 +
</html>
 +
    <!-- theme -->
 +
    <!-- word begin-->
 
= Basic Part ==
 
= Basic Part ==
 
<div>
 
<div>
Line 1,396: Line 1,533:
 
   </div>
 
   </div>
 
</div>
 
</div>
 +
 +
    <!-- word end -->
 +
<!-- themejs -->
 +
<html>
 +
<footer>
 +
    <script src="https://2016.igem.org/Team:FAFU-CHINA/page/js/bootstrap?action=raw&ctype=text/javascript"></script>
 +
<script>
 +
document.querySelector("head > link[rel='stylesheet']").remove();
 +
//$('#bars_item img').replaceWith($('#bars_item img').clone());
 +
 +
</script>
 +
 +
 +
  <script>
 +
if (!window.ga) {
 +
  (function(i,s,o,g,r,a,m){i['GoogleAnalyticsObject']=r;i[r]=i[r]||function(){
 +
  (i[r].q=i[r].q||[]).push(arguments)},i[r].l=1*new Date();a=s.createElement(o),
 +
  m=s.getElementsByTagName(o)[0];a.async=1;a.src=g;m.parentNode.insertBefore(a,m)
 +
  })(window,document,'script','//www.google-analytics.com/analytics.js','ga');
 +
 
 +
  window.ga('create', 'UA-42690126-5', 'auto');
 +
  window.ga('require', 'linkid', 'linkid.js');
 +
  window.ga('require', 'displayfeatures');
 +
  window.ga('send', 'pageview');
 +
} else {
 +
  window.ga('send', 'pageview', window.location.pathname+window.location.search);
 +
}
 +
 
 +
  </script>
 +
  <script>
 +
  $('#page-contents a').click(function() {
 +
    console.log(this.hash);
 +
    if (location.pathname.replace(/^\//,'') == this.pathname.replace(/^\//,'')
 +
        || location.hostname == this.hostname) {
 +
 +
        var target = $('[id="'+this.hash.substring(1)+'"]');
 +
        target = target.length ? target : $('[name="' + this.hash.slice(1) +'"]');
 +
          if (target.length) {
 +
            $('html,body').animate({
 +
                scrollTop: target.offset().top-70
 +
            }, 1000);
 +
            return false;
 +
        }
 +
    }
 +
  });
 +
  $(document.body).scrollspy({
 +
      target: '.bs-docs-sidebar',
 +
      offset: 71
 +
    });
 +
  function affix_sidebar () {
 +
      var $sideBar = $('.bs-docs-sidebar')
 +
 +
      $sideBar.affix({
 +
        offset: {
 +
          top: function () {
 +
            var offsetTop      = $sideBar.offset().top
 +
            var sideBarMargin  = parseInt($sideBar.children(0).css('margin-top'), 10)
 +
            var navOuterHeight = $('#index-nav-main').height()
 +
 +
            return (this.top = offsetTop - navOuterHeight - sideBarMargin)
 +
          },
 +
          bottom: function () {
 +
            return (this.bottom = $('.bs-docs-footer').outerHeight(true))
 +
          }
 +
        }
 +
      })
 +
    }
 +
  affix_sidebar();
 +
 +
  $("#searchform").submit(function (e){
 +
    $("#searchInput").val("FAFU"+$("#searchInput").val());
 +
  });
 +
  $('.reference>a, span.mw-cite-backlink > a').click(function() {
 +
    console.log(this.hash);
 +
    if (location.pathname.replace(/^\//,'') == this.pathname.replace(/^\//,'')
 +
        || location.hostname == this.hostname) {
 +
 +
        var target = $('[id="'+this.hash.substring(1)+'"]');
 +
        target = target.length ? target : $('[name="' + this.hash.slice(1) +'"]');
 +
          if (target.length) {
 +
            $('html,body').animate({
 +
                scrollTop: target.offset().top-70
 +
            }, 1000);
 +
            return false;
 +
        }
 +
    }
 +
  });
 +
 +
 +
  </script>
 +
</footer>
 +
</html>
 +
 +
<!-- themejs -->

Revision as of 11:43, 17 October 2016

Team FAFU-CHINA

Basic Part =

Cry4Aa4+Extended FMDV 2A-pChlamy_3

Gene Name

Cry4Aa4+Extended FMDV 2A

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

3673bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="263.20001220703125" src="http://b263.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/yfm7rDaljwf3tLDwN85uBcwarSLNpdnfMlUxh1G7pP4!/b/dAcBAAAAAAAA&bo=sAAIAQAAAAADAJw!" width="175.1999969482422">

<img height="294.3999938964844" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/O5V87mM7vgBQcBKlBbjOuO*vaJzRp9ohwsGNYf368uI!/b/dMYAAAAAAAAA&bo=nQAnAQAAAAADAJ4!" width="156.0">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA

Cry4Aa4+Extended FMDV 2A-pChlamy

Gene Name

Cry10Aa4+Extended FMDV 2A

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

2158bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/bQ8OOekpGDUmt.8vkQvJx0XUpn6PMh1VgaiF0dasSRg!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="308.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/LjSeZIndHm9zkUE6gT36bEx3VnYrEwCbYS7Aq.zBlqw!/b/dMcAAAAAAAAA&bo=7QA1AQAAAAADAPw!" width="236.8000030517578">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA

Cry11Aa4+ Extended FMDV 2A-pChlamy_3

Gene Name

Cry11Aa4+ Extended FMDV 2A

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

2071bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/d0.upRVhjL7OQjfkLGru*.dUocUXc4fDAJpeQEXjZe8!/b/dMYAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="308.0" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/0MvndoK9mmRt.kJuTifErWbDLJSPJui*8L*GLlyeGl8!/b/dMYAAAAAAAAA&bo=qAA1AQAAAAADALk!" width="167.1999969482422">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA

Cyt1-pChlamy_3

Gene Name

Cyt1

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

762bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/Sy.SKFU5edTQSIF.h2uwVTZjSgxUj3zmtAOCAGbAaZo!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="279.20001220703125" src="http://b264.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/xncHHhLWQz1aqSpaH4UR7R5bMZ4zFZ*PmV1nZxwVTQ8!/b/dAgBAAAAAAAA&bo=sAAYAQAAAAADAIw!" width="175.1999969482422">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA

Cyt2-pChlamy_3

Gene Name

Cyt1

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

801bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/d0mowbkc0M494OfDGRHIGtmKGQNkXPPWleN7nPIwSf0!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="280.0" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/irbPRy7*zARuesyKRBXZFk6ZWMGm44ZwwyjWYNaqYZA!/b/dAYBAAAAAAAA&bo=4QAZAQAAAAADANw!" width="224.0">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA

EGFP-pChlamy_3

Gene Name

06-EGFP（TAA）

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

732bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b260.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/kV2mqmj1q8v1ump215nFHpnjMUzwxqSu0DronaWKuIs!/b/dAQBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="296.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/aRGJqrgiOwOJDeH5hDKr.6c7zKU8VMfcgFHbm35PZ6I!/b/dMcAAAAAAAAA&bo=8AApAQAAAAADAP0!" width="239.1999969482422">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA

Cry4Aa4+Extended2A+Cyt2-pChlamy3

Gene Name

Cry4Aa4+Extended2A+Cyt2

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

4461bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/uN*b2mkL9gaipMv8XpN.4wpt8raBNnOQuGwbKC.w*vE!/b/dMcAAAAAAAAA&bo=qQD*AAAAAAADAHM!" width="168.8000030517578">

<img height="236.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/JI.J1CeIa*MhQu9aafo8T8eZeOr7zqxlK5q6W3NjbEA!/b/dMgAAAAAAAAA&bo=mADtAAAAAAADAFA!" width="151.1999969482422">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and BamHI

Lane 2: Plasmid DNA

Cry4Aa4+Extended2A+Cyt1-pChlamy3

Gene Name

Cry4Aa4+Extended2A+Cyt1-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

4422bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/pDvRNLD368siSdiKOdhck0A80upc24m2Aqj5qn.yyfY!/b/dMgAAAAAAAAA&bo=qQD*AAAAAAADAHM!" width="168.8000030517578">

<img height="280.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/LqdjCnlIs0z8YJ*6kbyj5ACC8gGPT6IlK2OdWcxc4T0!/b/dMcAAAAAAAAA&bo=rgAZAQAAAAADAJM!" width="173.60000610351562">

Lane M: DNA Marker

Lane 1: Plasmid digested by BamHI and KpnI

Lane 2: Plasmid DNA

Cry10Aa4+Extended2A+Cyt2-pChlamy3

Gene Name

Cry10Aa4+Extended2A+Cyt2-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

2946bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/H1*GSbsbhQbTR1eeNDiS8fA5AfLGIser.g5DKjHyGtU!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="264.0" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/JfiiusuOJGnfyciEpeNFbNeC5oqcVaFFkIBoPRxw6x0!/b/dMYAAAAAAAAA&bo=jQAJAQAAAAADAKA!" width="140.0">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA

Cry10Aa4+Extended2A+Cyt2-pChlamy3

Gene Name

Cry10Aa4+Extended2A+Cyt2-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

2946bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/9p5OCN4X0ji10gEBq68TdCUN93aBGaDnJIBTc5E0f3M!/b/dMcAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="273.6000061035156" src="http://b259.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/1zft.p5lOoX2yVoxccDyfQWcQjbGRb2SD*Zq97vSedo!/b/dAMBAAAAAAAA&bo=kQASAQAAAAADAKc!" width="144.8000030517578">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA

Cry10Aa4+Extended2A+Cyt1-pChlamy3

Gene Name

Cry10Aa4+Extended2A+Cyt1-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

2907bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/qchMjMQfSkqMMyaq5LhKw3ZuaGXoJRnrEvO0Slo0S1A!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="288.79998779296875" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/Rme26s7JjvB5bA9BlIrNu00W2reMmKwHkx9yaus6ikU!/b/dMgAAAAAAAAA&bo=zQAhAQAAAAADAMg!" width="204.8000030517578">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA

Cry11Aa4+Extended2A+Cyt2-pChlamy3

Gene Name

Cry11Aa4+Extended2A+Cyt2-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

2859bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/0LciDoc2Vues3GO6fYi7EnNqrsVS8mSF.qFDZziDpEA!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="258.3999938964844" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/hJNHsLw6HFbz9eR7eszEpW*U.RfWwyhYSlkMIPiWAbc!/b/dMYAAAAAAAAA&bo=wwADAQAAAAADAOQ!" width="194.39999389648438">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA

Cry11Aa4+Extended2A+Cyt1-pChlamy3

Gene Name

Cry11Aa4+Extended2A+Cyt1-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

2820bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b264.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/.39If2pAeG1h2OXRhp49InOl3WTN1asUm8AXJFwwqZs!/b/dAgBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="264.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/pZ5hAlXd5CMg3MJ.c2OE2MHz2ke4Ev3fW3jyPfdOUPU!/b/dMgAAAAAAAAA&bo=vQAJAQAAAAADAJA!" width="188.0">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA

Cry11Aa4+Extended2A+EGFP-pChlamy3

Gene Name

Cry11Aa4+Extended2A+EGFP-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

2790bp

Cloning Strategy

XbaI and PstI

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

<img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/cI8hqU6TnQQhJWgxrwgmQv0Mwgv4T3KwluBqsv0K9KA!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422">

<img height="259.20001220703125" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/94oeXP5iFtJKrKNWD4RQCmRJXVM2sO.rQztD9R2O50w!/b/dMYAAAAAAAAA&bo=qQAEAQAAAAADAIk!" width="168.8000030517578">

Lane M: DNA Marker

Lane 1: Plasmid digested by XbaI and PstI

Lane 2: Plasmid DNA