Difference between revisions of "Team:Oxford/Chemicals"

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<h2 id="TBE-Buffer">TBE Buffer pH 8.3</h2>
 
<h2 id="TBE-Buffer">TBE Buffer pH 8.3</h2>
−
   <p><u>Use:</u> General buffer for DNA experiments.<br>Made of three chemicals: Tris,borate and EDTA.</p>
+
<div class="div50" style="padding-top: 30px">
 +
   <p><u>Use:</u> General buffer for DNA experiments.<br>Made of three chemicals: Tris, borate and EDTA</p>
 
   <p>Non-Hazardous</p>
 
   <p>Non-Hazardous</p>
−
  <img src="https://static.igem.org/mediawiki/2016/4/47/T--Oxford--TBE.gif" style="float: right" width="40%"/>
+
</div>
−
 
+
<div class="div50">
 +
  <img src="https://static.igem.org/mediawiki/2016/4/47/T--Oxford--TBE.gif" style="float: right" width="90%"/>
 +
</div>
 +
<br style="clear: both">
 +
<p></p>
 
<!-- Copper Sulphate -->
 
<!-- Copper Sulphate -->
  
−
<h2 id="Copper-Sulfate-Pentahydrate">Copper Sulphate Pentahydrate:</h2>
+
<h2 id="Copper-Sulfate-Pentahydrate" style="align: left;">Copper Sulphate Pentahydrate</h2>
 +
<div class="div50" style="padding-top: 50px">
 
   <p><u>Use:</u> Induction of copper promoters and Cu2+ chelation assays.</p>
 
   <p><u>Use:</u> Induction of copper promoters and Cu2+ chelation assays.</p>
 
   <p><u>Signal word:</u> Warning</p>
 
   <p><u>Signal word:</u> Warning</p>
−
 
+
</div>
−
  <img src="https://static.igem.org/mediawiki/2016/b/b6/T--Oxford--CUSO4H2O.png" style="float: right" width="30%"/>
+
<div class="div50">
 +
  <img src="https://static.igem.org/mediawiki/2016/b/b6/T--Oxford--CUSO4H2O.png" style="float: right" width="80%"/>
 +
</div>
 +
<br style="clear: both">
 
   <p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
   <p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
   <ul>
 
   <ul>
Line 121: Line 130:
  
 
  <h2 id="Agarose">Agarose</h2>
 
  <h2 id="Agarose">Agarose</h2>
 +
<div class="div50">
 
   <p><u>Use:</u> DNA electrophoresis gel matrix</p>
 
   <p><u>Use:</u> DNA electrophoresis gel matrix</p>
 
   <p>Non-Hazardous</p>
 
   <p>Non-Hazardous</p>
−
  <img src="https://static.igem.org/mediawiki/2016/d/df/T--Oxford--Agarose.png" style="float: right" width="40%"/>
+
</div>
−
 
+
<div class="div50">
 +
  <img src="https://static.igem.org/mediawiki/2016/d/df/T--Oxford--Agarose.png" style="float: right" width="100%"/>
 +
</div>
 +
<br style="clear: both">
 +
<p></p>
 
<!-- Arabinose -->
 
<!-- Arabinose -->
  
 
<h2 id="L-Arabinose">L(+)-Arabinose</h2>
 
<h2 id="L-Arabinose">L(+)-Arabinose</h2>
 +
<div class="div50" style="padding-top: 50px">
 
<p><u>Use:</u> Induction of pBAD expression</p>
 
<p><u>Use:</u> Induction of pBAD expression</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
−
 
+
</div>
−
  <img src="https://static.igem.org/mediawiki/2016/a/af/T--Oxford--Arabinose.png" style="float: right" width="40%"/>
+
<div class="div50">
−
 
+
  <img src="https://static.igem.org/mediawiki/2016/a/af/T--Oxford--Arabinose.png" style="float: right" width="80%"/>
 +
</div>
 +
<br style="clear: both">
 +
<p></p>
  
 
<!-- Bathocuproinedisulfonic Acid Disodium salt -->
 
<!-- Bathocuproinedisulfonic Acid Disodium salt -->
  
 
<h2 id="BCA-Salt">Bathocuproinedisulfonic Acid Disodium Salt</h2>
 
<h2 id="BCA-Salt">Bathocuproinedisulfonic Acid Disodium Salt</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Chromophore in Cu+/Cu2+ detection assay</p>
 
<p><u>Use:</u> Chromophore in Cu+/Cu2+ detection assay</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
−
 
+
</div>
−
<img src= "https://static.igem.org/mediawiki/2016/6/64/T--Oxford--BCA.png" style="float: right"  width="40%">
+
<div class="div50">
 +
<img src= "https://static.igem.org/mediawiki/2016/6/64/T--Oxford--BCA.png" style="float: right"  width="90%">
 +
</div>
 +
<br style="clear: both">
 +
<p></p>
  
 
<!-- Ascorbate -->
 
<!-- Ascorbate -->
  
 
<h2 id="Sodium-Ascorbate">L(+)-Sodium Ascorbate</h2>
 
<h2 id="Sodium-Ascorbate">L(+)-Sodium Ascorbate</h2>
 +
<div class="div50" style="padding-top: 50px">
 
<p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay</p>
 
<p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/4/47/T--Oxford--Ascorbate.png" style="float: right" width="40%"/>
+
<div class="div50">
 +
<img src="https://static.igem.org/mediawiki/2016/4/47/T--Oxford--Ascorbate.png" style="float: right" width="90%"/>
 +
</div>
 +
<br style="clear: both">
 +
<p></p>
  
 
<!-- Glutathione -->
 
<!-- Glutathione -->
  
 
<h2 id="L-Glutathione">L-Glutathione</h2>
 
<h2 id="L-Glutathione">L-Glutathione</h2>
 +
<div class="div50" style="padding-top: 50px">
 
<p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay.</p>
 
<p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay.</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/d/dd/T--Oxford--Glutathione.png" style="float: right" width="40%"/>
+
<div class="div50">
 +
<img src="https://static.igem.org/mediawiki/2016/d/dd/T--Oxford--Glutathione.png" style="float: right" width="90%"/>
 +
</div>
 +
<br style="clear: both">
 +
<p></p>
  
 
<!-- Chloramphenicol -->
 
<!-- Chloramphenicol -->
  
 
<h2 id="Chloramphenicol">Chloramphenicol</h2>
 
<h2 id="Chloramphenicol">Chloramphenicol</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Antibiotic and positive selection marker for all plasmids except pBAD</p>
 
<p><u>Use:</u> Antibiotic and positive selection marker for all plasmids except pBAD</p>
 
<p><u>Signal word:</u> Danger</p>
 
<p><u>Signal word:</u> Danger</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/c/cd/T--Oxford--Chloramphenicol.png" style="float: right" width="40%"/>
+
<div class="div50">
−
 
+
<img src="https://static.igem.org/mediawiki/2016/c/cd/T--Oxford--Chloramphenicol.png" style="float: right" width="90%"/>
 +
</div>
 +
<br style="clear: both">
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 179: Line 215:
  
 
<h2 id="Ampicillin">Ampicillin</h2>
 
<h2 id="Ampicillin">Ampicillin</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Antibiotic and positive selection marker for pBAD.</p>
 
<p><u>Use:</u> Antibiotic and positive selection marker for pBAD.</p>
 
<p><u>Signal word:</u> Danger</p>
 
<p><u>Signal word:</u> Danger</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/8/8f/T--Oxford--Ampicillin.png" style="float: right" width="40%"/>
+
<div class="div50">
−
 
+
<img src="https://static.igem.org/mediawiki/2016/8/8f/T--Oxford--Ampicillin.png" style="float: right" width="90%"/>
 +
</div>
 +
<br style="clear: both">
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 203: Line 242:
  
 
<h2 id="Tris">Tris pH 7.0 and 8.0</h2>
 
<h2 id="Tris">Tris pH 7.0 and 8.0</h2>
 +
<div class="div50" style="padding-top: 30px">
 
<p><u>Use:</u> General buffer for protein and copper assays</p>
 
<p><u>Use:</u> General buffer for protein and copper assays</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/6/62/T--Oxford--TRIS.png" style="float: right" width="30%"/>
+
<div class="div50">
 +
<img src="https://static.igem.org/mediawiki/2016/6/62/T--Oxford--TRIS.png" style="float: right" width="65%"/>
 +
</div>
 +
<br style="clear: both">
 +
<p></p>
  
 
<!-- Glycerol -->
 
<!-- Glycerol -->
  
 
<h2 id="Glycerol">Glycerol</h2>
 
<h2 id="Glycerol">Glycerol</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer</p>
 
<p><u>Use:</u> Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/f/f6/T--Oxford--Glycerol.png" style="float: right" width="40%"/>
+
<div class="div50">
 +
<img src="https://static.igem.org/mediawiki/2016/f/f6/T--Oxford--Glycerol.png" style="float: right" width="90%"/>
 +
</div>
 +
<br style="clear: both">
 +
<p></p>
  
 
<!-- DTT -->
 
<!-- DTT -->
  
 
<h2 id="DL-Dithiothreitol">DL-Dithiothreitol</h2>
 
<h2 id="DL-Dithiothreitol">DL-Dithiothreitol</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay and protein purification</p>
 
<p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay and protein purification</p>
 
<p><u>Signal word:</u> Warning</p>
 
<p><u>Signal word:</u> Warning</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/e/ee/T--Oxford--DTT.png" style="float: right" width="40%"/>
+
<div class="div50">
−
 
+
<img src="https://static.igem.org/mediawiki/2016/e/ee/T--Oxford--DTT.png" style="float: right" width="90%"/>
 +
</div>
 +
<br style="clear: both">
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 240: Line 292:
  
 
<h2 id="Imidiazole">Imidazole</h2>
 
<h2 id="Imidiazole">Imidazole</h2>
 +
<div class="div50" style="padding-top: 55px">
 
<p><u>Use:</u> Elution of His-tagged target proteins from nickel bead column during protein purification</p>
 
<p><u>Use:</u> Elution of His-tagged target proteins from nickel bead column during protein purification</p>
 
<p><u>Signal word:</u> Danger</p>
 
<p><u>Signal word:</u> Danger</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/0/01/T--Oxford--Imidiazole.png" style="float: right" width="30%"/>
+
<div class="div50">
−
 
+
<img src="https://static.igem.org/mediawiki/2016/0/01/T--Oxford--Imidiazole.png" style="float: right" width="60%"/>
−
 
+
</div>
 +
<br style="clear: both">
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 263: Line 317:
  
 
<h2 id="Nickel-Affinity-Beads">Nickel Affinity Beads</h2>
 
<h2 id="Nickel-Affinity-Beads">Nickel Affinity Beads</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Selection of His-tagged target proteins during protein purification</p>
 
<p><u>Use:</u> Selection of His-tagged target proteins during protein purification</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/b/b9/T--Oxford--NiBEADS.gif" id=" Cont" style="float: right" width="40%"/>
+
<div class="div50">
 +
<img src="https://static.igem.org/mediawiki/2016/b/b9/T--Oxford--NiBEADS.gif" id=" Cont" style="float: right" width="90%"/>
 +
</div>
 
<br id="Cont">
 
<br id="Cont">
 +
<br style="clear: both">
 +
<p></p>
 +
 
<!-- Sodium Alginate -->
 
<!-- Sodium Alginate -->
  
 
<h2 id="Sodium-Alginate">Sodium Alginate</h2>
 
<h2 id="Sodium-Alginate">Sodium Alginate</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Major component of bacterial delivery beads</p>
 
<p><u>Use:</u> Major component of bacterial delivery beads</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/8/8a/T--Oxford--ALGINATE.png" style="float: right" width="55%"/>
+
<div class="div50">
 +
<img src="https://static.igem.org/mediawiki/2016/8/8a/T--Oxford--ALGINATE.png" style="float: right" width="90%"/>
 +
</div>
 +
<br style="clear: both">
 +
<p></p>
  
 
<!-- Crystal Violet -->
 
<!-- Crystal Violet -->
  
 
<h2 id="Crystal-Violet">Crystal Violet</h2>
 
<h2 id="Crystal-Violet">Crystal Violet</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Marker for bead quality assay</p>
 
<p><u>Use:</u> Marker for bead quality assay</p>
 
<p><u>Signal word:</u> Danger</p>
 
<p><u>Signal word:</u> Danger</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/f/f6/T--Oxford--Crystal_Violet.png" style="float: right" width="30%"/>
+
<div class="div50">
−
 
+
<img src="https://static.igem.org/mediawiki/2016/f/f6/T--Oxford--Crystal_Violet.png" style="float: right" width="70%"/>
 +
</div>
 +
<br style="clear: both">
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 304: Line 372:
  
 
<h2 id="Lysogeny-Broth">Lysogeny Broth</h2>
 
<h2 id="Lysogeny-Broth">Lysogeny Broth</h2>
−
<p><u>Use:</u> Media for general bacterial growth</p>
+
<div class="div50" style="padding-top: 50px">
 +
<p><u>Use:</u> Medium for general bacterial growth</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/a/ab/T--Oxford--LB.png" style="float: right" />
+
<div class="div50">
 +
<img src="https://static.igem.org/mediawiki/2016/a/ab/T--Oxford--LB.png" style="float: right" width="70%"/>
 +
</div>
 +
<br style="clear: both">
 +
<p></p>
  
 
<!-- Phosphate buffered saline -->
 
<!-- Phosphate buffered saline -->
  
 
<h2 id="Phosphate-Buffered-Saline">Phosphate Buffered Saline pH 7.4</h2>
 
<h2 id="Phosphate-Buffered-Saline">Phosphate Buffered Saline pH 7.4</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Dilution buffer for flow cytometry experiments</p>
 
<p><u>Use:</u> Dilution buffer for flow cytometry experiments</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/c/c1/T--Oxford--PBS.gif " style="float: right" width="40%"/>
+
<div class="div50">
 +
<img src="https://static.igem.org/mediawiki/2016/c/c1/T--Oxford--PBS.gif " style="float: right" width="65%"/>
 +
</div>
 +
<br style="clear: both">
 +
<p></p>
  
 
<!-- SDS -->
 
<!-- SDS -->
  
 
<h2 id="Sodium-Dodecyl-Sulfate">Sodium Dodecyl Sulphate</h2>
 
<h2 id="Sodium-Dodecyl-Sulfate">Sodium Dodecyl Sulphate</h2>
 +
 +
<div class="div50">
 
<p><u>Use:</u> Detergent in protein identification gels</p>
 
<p><u>Use:</u> Detergent in protein identification gels</p>
 
<p><u>Signal word:</u> Danger</p>
 
<p><u>Signal word:</u> Danger</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/1/15/T--Oxford--SDS.jpg" class="rotate" style="float: right" width="50%"/>
+
<div class="div50">
−
 
+
<img src="https://static.igem.org/mediawiki/2016/1/15/T--Oxford--SDS.jpg" style="float: right" width="70%"/>
 +
</div>
 +
<br style="clear: both">
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 347: Line 429:
  
 
<h2 id="Ethanol">Ethanol 100%</h2>
 
<h2 id="Ethanol">Ethanol 100%</h2>
 +
<div class="div50">
 
<p><u>Use:</u> solvent for chloramphenicol</p>
 
<p><u>Use:</u> solvent for chloramphenicol</p>
 
<p><u>Signal word:</u> Danger</p>
 
<p><u>Signal word:</u> Danger</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/b/b2/T--Oxford--Ethanol.png" style="float: right" width="60%"/>
+
<div class="div50">
−
 
+
<img src="https://static.igem.org/mediawiki/2016/b/b2/T--Oxford--Ethanol.png" style="float: right; padding-top: 30px" width="60%"/>
 +
</div>
 +
<br style="clear: both">
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 368: Line 453:
  
 
<h2 id="Transfection-Buffer-I">Transfection Buffer I (Manganese[II] Chloride)</h2>
 
<h2 id="Transfection-Buffer-I">Transfection Buffer I (Manganese[II] Chloride)</h2>
 +
<div class="div50">
 
<p><u>Use:</u> First soaking buffer in creating competent E. coli cells</p>
 
<p><u>Use:</u> First soaking buffer in creating competent E. coli cells</p>
 
<p><u>Signal word:</u> Danger</p>
 
<p><u>Signal word:</u> Danger</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/f/fa/T--Oxford--MnCl.png" style="float: right"/>
+
<div class="div50">
−
 
+
<img src="https://static.igem.org/mediawiki/2016/f/fa/T--Oxford--MnCl.png" style="float: right" width="60%"/>
 +
</div>
 +
<br style="clear: both">
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<ul>
 
<ul>

Revision as of 17:51, 17 October 2016

iGEM Oxford 2016 - Cure for Copper

Chemicals

Ethidium Bromide

Use: Staining DNA electrophoresis gels.

Signal word: Danger


Hazard statement(s)

  • H302 Harmful if swallowed.
  • H330 Fatal if inhaled.
  • H341 Suspected of causing genetic defects.

Precautionary statement(s)

  • P260 Do not breathe dust/fume/gas/mist/vapours/spray.
  • P281 Use personal protective equipment as required.
  • P284 Wear respiratory protection.
  • P310 Immediately call a POISON CENTER or doctor/ physician.

TBE Buffer pH 8.3

Use: General buffer for DNA experiments.
Made of three chemicals: Tris, borate and EDTA

Non-Hazardous


Copper Sulphate Pentahydrate

Use: Induction of copper promoters and Cu2+ chelation assays.

Signal word: Warning


Hazard statement(s)

  • H302 Harmful if swallowed.
  • H315 Causes skin irritation.
  • H319 Causes serious eye irritation.
  • H410 Very toxic to aquatic life with long lasting effects.

Precautionary statement(s)

  • P273 Avoid release to the environment.
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes.Remove contact lenses, if present and easy to do. Continue rinsing.
  • P501 Dispose of contents/container to an approved waste disposal plant.

Agarose

Use: DNA electrophoresis gel matrix

Non-Hazardous


L(+)-Arabinose

Use: Induction of pBAD expression

Non-Hazardous


Bathocuproinedisulfonic Acid Disodium Salt

Use: Chromophore in Cu+/Cu2+ detection assay

Non-Hazardous


L(+)-Sodium Ascorbate

Use: Reducing agent in Cu+/Cu2+ detection assay

Non-Hazardous


L-Glutathione

Use: Reducing agent in Cu+/Cu2+ detection assay.

Non-Hazardous


Chloramphenicol

Use: Antibiotic and positive selection marker for all plasmids except pBAD

Signal word: Danger


Hazard statement(s)

  • H350 May cause cancer

Precautionary statement(s)

  • P201 Obtain special instructions before use.
  • P308 + P313 If exposed or concerned: Get medical advice/attention.

Ampicillin

Use: Antibiotic and positive selection marker for pBAD.

Signal word: Danger


Hazard statement(s)

  • H315 Causes skin irritation
  • H317 May cause an allergic skin reaction
  • H319 Causes serious eye irritation
  • H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled
  • H335 May cause respiratory irritation

Precautionary statement(s)

  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P342 + P311 If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician

Tris pH 7.0 and 8.0

Use: General buffer for protein and copper assays

Non-Hazardous


Glycerol

Use: Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer

Non-Hazardous


DL-Dithiothreitol

Use: Reducing agent in Cu+/Cu2+ detection assay and protein purification

Signal word: Warning


Hazard statement(s)

  • H302 Harmful if swallowed
  • H315 Causes skin irritation
  • H319 Causes serious eye irritation
  • H335 May cause respiratory irritation

Precautionary statement(s)

  • P261 Avoid breathing dust
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

Imidazole

Use: Elution of His-tagged target proteins from nickel bead column during protein purification

Signal word: Danger


Hazard statement(s)

  • H302 Harmful if swallowed
  • H314 Causes severe skin burns and eye damage
  • H360D May damage the unborn child

Precautionary statement(s)

  • P201 Obtain special instructions before use
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P310 Immediately call a POISON CENTER or doctor/physician

Nickel Affinity Beads

Use: Selection of His-tagged target proteins during protein purification

Non-Hazardous



Sodium Alginate

Use: Major component of bacterial delivery beads

Non-Hazardous


Crystal Violet

Use: Marker for bead quality assay

Signal word: Danger


Hazard statement(s)

  • H302 Harmful if swallowed
  • H318 Causes serious eye damage
  • H350 May cause cancer
  • H410 Very toxic to aquatic life with long lasting effects

Precautionary statement(s)

  • P201 Obtain special instructions before use
  • P273 Avoid release to the environment
  • P280 Wear protective gloves/ eye protection/ face protection
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P308 + P313 If exposed or concerned: Get medical advice/attention
  • P501 Dispose of contents/container to an approved waste disposal plant

Lysogeny Broth

Use: Medium for general bacterial growth

Non-Hazardous


Phosphate Buffered Saline pH 7.4

Use: Dilution buffer for flow cytometry experiments

Non-Hazardous


Sodium Dodecyl Sulphate

Use: Detergent in protein identification gels

Signal word: Danger


Hazard statement(s)

  • H228 Flammable solid
  • H302 + H332 Harmful if swallowed or if inhaled
  • H315 Causes skin irritation
  • H318 Causes serious eye damage
  • H335 May cause respiratory irritation
  • H412 Harmful to aquatic life with long lasting effects

Precautionary statement(s)

  • P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P280 Wear eye protection/face protection
  • P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth
  • P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor
  • P370 + P378 In case of fire: Use dry powder or dry sand to extinguish

Ethanol 100%

Use: solvent for chloramphenicol

Signal word: Danger


Hazard statement(s)

  • H225 Highly flammable liquid and vapour
  • H319 Causes serious eye irritation

Precautionary statement(s)

  • P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P370 + P378 In case of fire: Use dry powder or dry sand to extinguish
  • P403 + P235 Store in a well-ventilated place. Keep cool

Transfection Buffer I (Manganese[II] Chloride)

Use: First soaking buffer in creating competent E. coli cells

Signal word: Danger


Hazard statement(s)

  • H302 Harmful if swallowed
  • H318 Causes serious eye damage
  • H373 May cause damage to organs (Brain) through prolonged or repeated exposure if inhaled
  • H411 Toxic to aquatic life with long lasting effects

Precautionary statement(s)

  • P273 Avoid release to the environment
  • P280 Wear eye protection/ face protection
  • P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. Rinse mouth
  • P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician
  • P391 Collect spillage
  • P501 Dispose of contents/container to an approved waste disposal plant

Transfection Buffer II

Use: Second soaking buffer in creating competent E. coli cells

Non-Hazardous

Chitosan

Use: Coating for bacterial delivery beads

Non-Hazardous

InstantBlue

Use: Protein stain for protein identification gels

Signal word: Warning

Hazard statement(s)

  • H290 May be corrosive to metals
  • H315 Causes skin irritation
  • H319 Causes serious eye irritation

Precautionary statement(s)

  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

NEB Purple Loading Dye

Use: Stabilizer and marker for DNA gel electrophoresis

Non-Hazardous

Roche cOmplete EDTA-free Protease Inhibitor Cocktail

Use: Prevention of protein cleavage during protein purification

Signal word: Danger

Hazard statement(s)

  • H314 Causes severe skin burns and eye damage Skin corrosion/irritation

Precautionary statement(s)

  • P260 Do not breathe dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P301 + P330 + P331 IF SWALLOWED: Rinse mouth. Do NOT induce vomiting
  • P303 + P361 + P353 IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower
  • P304 + P340 + P310 IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. Immediately call a POISON CENTER or doctor/physician
  • P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician

Hydrochloric Acid 1M

Use: Simulation of stomach acid conditions in bead experiments

Signal word: Warning

Hazard statement(s)

  • H290 May be corrosive to metals

Sodium Hydroxide

Use: Simulation of duodenum conditions in bead experiments

Signal word: Danger

Hazard statement(s)

  • H290 May be corrosive to metals
  • H314 Causes severe skin burns and eye damage

Precautionary statement(s)

  • P260 Do not breathe dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
  • P304 + P340 + P310 IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

Monobasic Sodium Phosphate

Use: Simulation of duodenum conditions in bead experiments

Non-Hazardous

Glacial Acetic Acid

Use: Bead experiments

Signal word: Danger

Hazard statement(s)

  • H226 Flammable liquid and vapour
  • H314 Causes severe skin burns and eye damage

Precautionary statement(s)

  • P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
  • P260 Do not breathe dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P370 + P378 In case of fire: Use dry powder or dry sand to extinguish

Calcium Chloride

Use: Forming beads from alginate. Ca2+ causes alginate phase change

Signal word: Warning

Hazard statement(s)

  • H319 Causes serious eye irritation

Precautionary statement(s)

  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing