Difference between revisions of "Team:Oxford/Chemicals"

Line 404: Line 404:
 
</div>
 
</div>
 
<div class="div50">
 
<div class="div50">
−
<img src="https://static.igem.org/mediawiki/2016/1/15/T--Oxford--SDS.jpg" style="float: right" width="70%"/>
+
<img src="https://static.igem.org/mediawiki/2016/1/15/T--Oxford--SDS.jpg" style="float: right" width="90%"/>
 
</div>
 
</div>
 
<br style="clear: both">
 
<br style="clear: both">
Line 481: Line 481:
  
 
<h2 id="Transfection-Buffer-II">Transfection Buffer II</h2>
 
<h2 id="Transfection-Buffer-II">Transfection Buffer II</h2>
 +
 
<p><u>Use:</u> Second soaking buffer in creating competent E. coli cells</p>
 
<p><u>Use:</u> Second soaking buffer in creating competent E. coli cells</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
Line 487: Line 488:
  
 
<h2 id="Chitosan">Chitosan</h2>
 
<h2 id="Chitosan">Chitosan</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Coating for bacterial delivery beads</p>
 
<p><u>Use:</u> Coating for bacterial delivery beads</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/4/4a/T--Oxford--Chitosan.png" class="rotate" style="float: right" width="45%"/>
+
<div class="div50">
 +
<img src="https://static.igem.org/mediawiki/2016/4/4a/T--Oxford--Chitosan.png" style="float: right" width="90%"/>
 +
</div>
 +
<br style="clear: both">
 +
<p></p>
  
 
<!-- Instant Blue -->
 
<!-- Instant Blue -->
  
 
<h2 id="Instant-Blue">InstantBlue</h2>
 
<h2 id="Instant-Blue">InstantBlue</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Protein stain for protein identification gels</p>
 
<p><u>Use:</u> Protein stain for protein identification gels</p>
 
<p><u>Signal word:</u> Warning</p>
 
<p><u>Signal word:</u> Warning</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/9/9c/T--Oxford--InstantBlue.png" style="float: right" width="50%"/>
+
<div class="div50">
−
 
+
<img src="https://static.igem.org/mediawiki/2016/9/9c/T--Oxford--InstantBlue.png" style="float: right" width="90%"/>
 +
</div>
 +
<br style="clear: both">
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 514: Line 523:
  
 
<h2 id="NEB-Purple-Loading-Dye">NEB Purple Loading Dye</h2>
 
<h2 id="NEB-Purple-Loading-Dye">NEB Purple Loading Dye</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Stabilizer and marker for DNA gel electrophoresis</p>
 
<p><u>Use:</u> Stabilizer and marker for DNA gel electrophoresis</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
−
 
+
</div>
 +
<div class="div50">
 
<img src="https://static.igem.org/mediawiki/2016/8/88/T--Oxford--pURPLE_LOADING_DYE.jpg" style="float: right"/>
 
<img src="https://static.igem.org/mediawiki/2016/8/88/T--Oxford--pURPLE_LOADING_DYE.jpg" style="float: right"/>
 +
</div>
 +
<br style="clear: both">
 +
<p></p>
  
 
<!-- Protease Inhibitors -->
 
<!-- Protease Inhibitors -->
  
 
<h2 id="Protease-Inhibitors">Roche cOmplete EDTA-free Protease Inhibitor Cocktail</h2>
 
<h2 id="Protease-Inhibitors">Roche cOmplete EDTA-free Protease Inhibitor Cocktail</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Prevention of protein cleavage during protein purification</p>
 
<p><u>Use:</u> Prevention of protein cleavage during protein purification</p>
 
<p><u>Signal word:</u> Danger</p>
 
<p><u>Signal word:</u> Danger</p>
−
 
+
</div>
 +
<div class="div50">
 
<img src="https://static.igem.org/mediawiki/2016/6/68/T--Oxford--protease_inhibitors.jpg" style="float: right"/>
 
<img src="https://static.igem.org/mediawiki/2016/6/68/T--Oxford--protease_inhibitors.jpg" style="float: right"/>
−
 
+
</div>
 +
<br style="clear: both">
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 542: Line 559:
  
 
<!-- HCL -->
 
<!-- HCL -->
−
<section>
 
 
<h2 id="Hydrochloric-Acid">Hydrochloric Acid 1M</h2>
 
<h2 id="Hydrochloric-Acid">Hydrochloric Acid 1M</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Simulation of stomach acid conditions in bead experiments</p>
 
<p><u>Use:</u> Simulation of stomach acid conditions in bead experiments</p>
 
<p><u>Signal word:</u> Warning</p>
 
<p><u>Signal word:</u> Warning</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/f/f5/T--Oxford--HCL.png" style="float: right" width="40%"/>
+
<div class="div50">
−
 
+
<img src="https://static.igem.org/mediawiki/2016/f/f5/T--Oxford--HCL.png" style="float: right" width="90%"/>
−
 
+
</div>
 +
<br style="clear: both">
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
 
<li>H290 May be corrosive to metals</li>
 
<li>H290 May be corrosive to metals</li>
 
</ul>
 
</ul>
−
</section>
 
  
 
<!-- NaOH -->
 
<!-- NaOH -->
  
 
<h2 id="Sodium-Hydroxide">Sodium Hydroxide</h2>
 
<h2 id="Sodium-Hydroxide">Sodium Hydroxide</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Simulation of duodenum conditions in bead experiments</p>
 
<p><u>Use:</u> Simulation of duodenum conditions in bead experiments</p>
 
<p><u>Signal word:</u> Danger</p>
 
<p><u>Signal word:</u> Danger</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/d/d7/T--Oxford--SODIUM_HYDROXIDE.png" style="float: right" width="40%"/>
+
<div class="div50">
−
 
+
<img src="https://static.igem.org/mediawiki/2016/d/d7/T--Oxford--SODIUM_HYDROXIDE.png" style="float: right" width="90%"/>
−
 
+
</div>
 +
<br style="clear: both">
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 582: Line 601:
  
 
<h2 id="Monobasic-Sodium-Phosphate">Monobasic Sodium Phosphate</h2>
 
<h2 id="Monobasic-Sodium-Phosphate">Monobasic Sodium Phosphate</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Simulation of duodenum conditions in bead experiments</p>
 
<p><u>Use:</u> Simulation of duodenum conditions in bead experiments</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
−
 
+
</div>
 +
<div class="div50">
 
<img src="https://static.igem.org/mediawiki/2016/b/ba/T--Oxford--MBNaP.png" style="float: right"/>
 
<img src="https://static.igem.org/mediawiki/2016/b/ba/T--Oxford--MBNaP.png" style="float: right"/>
−
 
+
</div>
 +
<br style="clear: both">
 +
<p></p>
  
 
<!-- Glacial Acetic ACid -->
 
<!-- Glacial Acetic ACid -->
  
 
<h2 id="Glacial-Acetic-Acid">Glacial Acetic Acid</h2>
 
<h2 id="Glacial-Acetic-Acid">Glacial Acetic Acid</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Bead experiments</p>
 
<p><u>Use:</u> Bead experiments</p>
 
<p><u>Signal word:</u> Danger</p>
 
<p><u>Signal word:</u> Danger</p>
−
 
+
</div>
−
<img src="https://static.igem.org/mediawiki/2016/1/16/T--Oxford--Acetic_Acid.png" style="float: right" width="40%"/>
+
<div class="div50">
−
 
+
<img src="https://static.igem.org/mediawiki/2016/1/16/T--Oxford--Acetic_Acid.png" style="float: right" width="80%"/>
 +
</div>
 +
<br style="clear: both">
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 614: Line 640:
  
 
<h2 id="Calcium-Chloride">Calcium Chloride</h2>
 
<h2 id="Calcium-Chloride">Calcium Chloride</h2>
 +
<div class="div50">
 
<p><u>Use:</u> Forming beads from alginate.  Ca2+ causes alginate phase change</p>
 
<p><u>Use:</u> Forming beads from alginate.  Ca2+ causes alginate phase change</p>
 
<p><u>Signal word:</u> Warning</p>
 
<p><u>Signal word:</u> Warning</p>
−
 
+
</div>
 +
<div class="div50">
 
<img src="https://static.igem.org/mediawiki/2016/8/85/T--Oxford--CACL2.png" style="float: right"/>
 
<img src="https://static.igem.org/mediawiki/2016/8/85/T--Oxford--CACL2.png" style="float: right"/>
−
 
+
</div>
 +
<br style="clear: both">
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<ul>
 
<ul>

Revision as of 18:06, 17 October 2016

iGEM Oxford 2016 - Cure for Copper

Chemicals

Ethidium Bromide

Use: Staining DNA electrophoresis gels.

Signal word: Danger


Hazard statement(s)

  • H302 Harmful if swallowed.
  • H330 Fatal if inhaled.
  • H341 Suspected of causing genetic defects.

Precautionary statement(s)

  • P260 Do not breathe dust/fume/gas/mist/vapours/spray.
  • P281 Use personal protective equipment as required.
  • P284 Wear respiratory protection.
  • P310 Immediately call a POISON CENTER or doctor/ physician.

TBE Buffer pH 8.3

Use: General buffer for DNA experiments.
Made of three chemicals: Tris, borate and EDTA

Non-Hazardous


Copper Sulphate Pentahydrate

Use: Induction of copper promoters and Cu2+ chelation assays.

Signal word: Warning


Hazard statement(s)

  • H302 Harmful if swallowed.
  • H315 Causes skin irritation.
  • H319 Causes serious eye irritation.
  • H410 Very toxic to aquatic life with long lasting effects.

Precautionary statement(s)

  • P273 Avoid release to the environment.
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes.Remove contact lenses, if present and easy to do. Continue rinsing.
  • P501 Dispose of contents/container to an approved waste disposal plant.

Agarose

Use: DNA electrophoresis gel matrix

Non-Hazardous


L(+)-Arabinose

Use: Induction of pBAD expression

Non-Hazardous


Bathocuproinedisulfonic Acid Disodium Salt

Use: Chromophore in Cu+/Cu2+ detection assay

Non-Hazardous


L(+)-Sodium Ascorbate

Use: Reducing agent in Cu+/Cu2+ detection assay

Non-Hazardous


L-Glutathione

Use: Reducing agent in Cu+/Cu2+ detection assay.

Non-Hazardous


Chloramphenicol

Use: Antibiotic and positive selection marker for all plasmids except pBAD

Signal word: Danger


Hazard statement(s)

  • H350 May cause cancer

Precautionary statement(s)

  • P201 Obtain special instructions before use.
  • P308 + P313 If exposed or concerned: Get medical advice/attention.

Ampicillin

Use: Antibiotic and positive selection marker for pBAD.

Signal word: Danger


Hazard statement(s)

  • H315 Causes skin irritation
  • H317 May cause an allergic skin reaction
  • H319 Causes serious eye irritation
  • H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled
  • H335 May cause respiratory irritation

Precautionary statement(s)

  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P342 + P311 If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician

Tris pH 7.0 and 8.0

Use: General buffer for protein and copper assays

Non-Hazardous


Glycerol

Use: Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer

Non-Hazardous


DL-Dithiothreitol

Use: Reducing agent in Cu+/Cu2+ detection assay and protein purification

Signal word: Warning


Hazard statement(s)

  • H302 Harmful if swallowed
  • H315 Causes skin irritation
  • H319 Causes serious eye irritation
  • H335 May cause respiratory irritation

Precautionary statement(s)

  • P261 Avoid breathing dust
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

Imidazole

Use: Elution of His-tagged target proteins from nickel bead column during protein purification

Signal word: Danger


Hazard statement(s)

  • H302 Harmful if swallowed
  • H314 Causes severe skin burns and eye damage
  • H360D May damage the unborn child

Precautionary statement(s)

  • P201 Obtain special instructions before use
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P310 Immediately call a POISON CENTER or doctor/physician

Nickel Affinity Beads

Use: Selection of His-tagged target proteins during protein purification

Non-Hazardous



Sodium Alginate

Use: Major component of bacterial delivery beads

Non-Hazardous


Crystal Violet

Use: Marker for bead quality assay

Signal word: Danger


Hazard statement(s)

  • H302 Harmful if swallowed
  • H318 Causes serious eye damage
  • H350 May cause cancer
  • H410 Very toxic to aquatic life with long lasting effects

Precautionary statement(s)

  • P201 Obtain special instructions before use
  • P273 Avoid release to the environment
  • P280 Wear protective gloves/ eye protection/ face protection
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P308 + P313 If exposed or concerned: Get medical advice/attention
  • P501 Dispose of contents/container to an approved waste disposal plant

Lysogeny Broth

Use: Medium for general bacterial growth

Non-Hazardous


Phosphate Buffered Saline pH 7.4

Use: Dilution buffer for flow cytometry experiments

Non-Hazardous


Sodium Dodecyl Sulphate

Use: Detergent in protein identification gels

Signal word: Danger


Hazard statement(s)

  • H228 Flammable solid
  • H302 + H332 Harmful if swallowed or if inhaled
  • H315 Causes skin irritation
  • H318 Causes serious eye damage
  • H335 May cause respiratory irritation
  • H412 Harmful to aquatic life with long lasting effects

Precautionary statement(s)

  • P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P280 Wear eye protection/face protection
  • P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth
  • P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor
  • P370 + P378 In case of fire: Use dry powder or dry sand to extinguish

Ethanol 100%

Use: solvent for chloramphenicol

Signal word: Danger


Hazard statement(s)

  • H225 Highly flammable liquid and vapour
  • H319 Causes serious eye irritation

Precautionary statement(s)

  • P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P370 + P378 In case of fire: Use dry powder or dry sand to extinguish
  • P403 + P235 Store in a well-ventilated place. Keep cool

Transfection Buffer I (Manganese[II] Chloride)

Use: First soaking buffer in creating competent E. coli cells

Signal word: Danger


Hazard statement(s)

  • H302 Harmful if swallowed
  • H318 Causes serious eye damage
  • H373 May cause damage to organs (Brain) through prolonged or repeated exposure if inhaled
  • H411 Toxic to aquatic life with long lasting effects

Precautionary statement(s)

  • P273 Avoid release to the environment
  • P280 Wear eye protection/ face protection
  • P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. Rinse mouth
  • P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician
  • P391 Collect spillage
  • P501 Dispose of contents/container to an approved waste disposal plant

Transfection Buffer II

Use: Second soaking buffer in creating competent E. coli cells

Non-Hazardous

Chitosan

Use: Coating for bacterial delivery beads

Non-Hazardous


InstantBlue

Use: Protein stain for protein identification gels

Signal word: Warning


Hazard statement(s)

  • H290 May be corrosive to metals
  • H315 Causes skin irritation
  • H319 Causes serious eye irritation

Precautionary statement(s)

  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

NEB Purple Loading Dye

Use: Stabilizer and marker for DNA gel electrophoresis

Non-Hazardous


Roche cOmplete EDTA-free Protease Inhibitor Cocktail

Use: Prevention of protein cleavage during protein purification

Signal word: Danger


Hazard statement(s)

  • H314 Causes severe skin burns and eye damage Skin corrosion/irritation

Precautionary statement(s)

  • P260 Do not breathe dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P301 + P330 + P331 IF SWALLOWED: Rinse mouth. Do NOT induce vomiting
  • P303 + P361 + P353 IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower
  • P304 + P340 + P310 IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. Immediately call a POISON CENTER or doctor/physician
  • P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician

Hydrochloric Acid 1M

Use: Simulation of stomach acid conditions in bead experiments

Signal word: Warning


Hazard statement(s)

  • H290 May be corrosive to metals

Sodium Hydroxide

Use: Simulation of duodenum conditions in bead experiments

Signal word: Danger


Hazard statement(s)

  • H290 May be corrosive to metals
  • H314 Causes severe skin burns and eye damage

Precautionary statement(s)

  • P260 Do not breathe dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
  • P304 + P340 + P310 IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

Monobasic Sodium Phosphate

Use: Simulation of duodenum conditions in bead experiments

Non-Hazardous


Glacial Acetic Acid

Use: Bead experiments

Signal word: Danger


Hazard statement(s)

  • H226 Flammable liquid and vapour
  • H314 Causes severe skin burns and eye damage

Precautionary statement(s)

  • P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
  • P260 Do not breathe dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P370 + P378 In case of fire: Use dry powder or dry sand to extinguish

Calcium Chloride

Use: Forming beads from alginate. Ca2+ causes alginate phase change

Signal word: Warning


Hazard statement(s)

  • H319 Causes serious eye irritation

Precautionary statement(s)

  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing