To meet the criteria of the Gold Medal, we submitted BBa_K1949060
and we characterized BBa_R0071, BBa_C0071, BBa_C0171, BBa_K1529300, BBa_K1529310, BBa_K1096001 and BBa_K1096002.

To meet the criteria of the Silver Medal, we submitted BBa_K1949050, BBa_K1949052, BBa_K1949030 and BBa_K1949032.

To meet the criteria of the Bronze Medal, we submitted BBa_K1949000, BBa_K1949001, BBa_K1949100, BBa_K1949101 and BBa_K1949102.

BBa_K1949060 meets the Gold Medal criteria!

We simulated our final genetic circuits and found that the circuits did not work, because Prhl activity was too weak compared to Plux. (see the Model page and the AHL Only Assay page). We therefore considered using the improved Prhl (BBa_K1529310, BBa_K1529300) established by iGEM 2014 Tokyo_Tech, but we noticed that they were inappropriate for two reasons (see the Discussion). Then, we decided to improve Prhl Promoter and obtain our original improved Prhl (Noticeable Mutant) (BBa_K1949060), hereafter referred to as Prhl(NM), that suited our goal.

Our purpose is to create strong Prhl for our final genetic circuits.

This experiment consists of the three parts below.

1. Improved Prhl by iGEM 2014 Tokyo_Tech and characterize RhlR assay.

2. Improvement of the wild type Prhl.

3. Comparison of the improved Prhl by iGEM 2014 Tokyo_Tech to our original improved Prhl

The past improved Prhl did not suit for our final circuits and we could construct the improved Prhl appropriate to our final circuits.

For more information, see <- なにこれ

BBa_K1949050 meets the Silver Medal criteria!

The Prince coli expresses AmiE protein, and Snow White coli recovers from its apparent death and wakes up again. We tested the function of AmiE protein that influences the end of the story.

Our objective is to characterize the function of AmiE protein. We prepared three samples shown below. When we tested the AmiE degradation ability with these samples, the results show that C4HSL is not degraded by AmiE, but 3OC12HSL is degraded by AmiE.

• PBAD-rbs-amiE(pSB6A1)

• Ptet-rbs-luxR-tt-Plux-rbs-gfp (pSB6A1)

• Ptet-rbs-luxR-tt-Plux-rbs-gfp (pSB6A1)

BBa_K1949102 meets the Bronze Medal criteria!

The biggest attraction of the TA system is that it is able to control cell growth and synthesis of protein. In this experiment, mazE expression was induced by the addition of IPTG(2 mM) after mazF expression was induced by the addition of arabinose(0.02%). As a result, it was able to resuscitate from a state of being inhibited cell growth. We named this experiment as "Stop & Go" because it was to resuscitate growth from inhibiting cell growth.