Sunday, August 7th
Tasks:
Sara & Tyler
- Picked colonies on MCherry 1-5 plates from 8/4 and MCherry 1 from 8/5
- Because Tet plates were contaminated, used a higher concentration of tetracyline than previous, 20 uL of 5 mg/ml, working concentration 50 ug/ml 2 ml cultures of each
- MCherry and GFP storage vector plates had lawns on them, and lawns on the negative control--plates were irregularly poured or antibiotic is bad, could be both
- Antibiotic is also from 2011
- Minipreps of Cas9 and GFP storage vector cultures- nanodrop notes
Tube
Concentration (ng/uL)
260/280
260/230
Cas9 1:2 from 8/5
52
1.97
1.90
Cas9 1:3 from 8/1
241
1.90
2.30
Cas9 1:3 from 8/4
152
1.90
2.22
GFP 1 storage in pSB1T3
74
1.87
1.44
GFP 2 storage in pSB1T3 / reblank
63/41
1.82/1.96
1.07/1.78
GFP 3 storage in pSB1T3
9.4
1.73
0..82
Sara & Tyler
- Picked colonies on MCherry 1-5 plates from 8/4 and MCherry 1 from 8/5
- Because Tet plates were contaminated, used a higher concentration of tetracyline than previous, 20 uL of 5 mg/ml, working concentration 50 ug/ml 2 ml cultures of each
- MCherry and GFP storage vector plates had lawns on them, and lawns on the negative control--plates were irregularly poured or antibiotic is bad, could be both
- Antibiotic is also from 2011
- Minipreps of Cas9 and GFP storage vector cultures- nanodrop notes
| Tube | Concentration (ng/uL) | 260/280 | 260/230 |
|---|---|---|---|
| Cas9 1:2 from 8/5 | 52 | 1.97 | 1.90 |
| Cas9 1:3 from 8/1 | 241 | 1.90 | 2.30 |
| Cas9 1:3 from 8/4 | 152 | 1.90 | 2.22 |
| GFP 1 storage in pSB1T3 | 74 | 1.87 | 1.44 |
| GFP 2 storage in pSB1T3 / reblank | 63/41 | 1.82/1.96 | 1.07/1.78 |
| GFP 3 storage in pSB1T3 | 9.4 | 1.73 | 0..82 |
