Difference between revisions of "Team:Northwestern/09 10"

 
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Latest revision as of 15:35, 18 October 2016

Notebook

Saturday, September 10th

Tasks:

Jordan & Tyler

  • Ran a Gibson on Cas9-Lrz-SS and TorA (with PCR-added homology)
  • Made 5 mL overnight cultures of:
    • 10 colonies from Cas9-DsbA Gibson assembly product with PCR-added homology to insert
    • 2 colonies from Cas9-DsbA Gibson assembly product with original DsbA gBlock
  • Gibson reaction of TorA-Cas9:
    • 3.3µL water
    • 1.16 µL backbone
    • 0.54µL insert
    • 5µL Gibson Mix
  • Miniprep Results: