Difference between revisions of "Team:Nagahama/Protocol"

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Medium
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LB medium (100 mL liquid)
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1.Measure 1g Tripton
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2.Measure 0.5g Yeast Extract
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3.Measure 1g Nacl
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4.Add 100mL H2O
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5.autoclave(121℃ 20min)
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<Br>
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2×YT medium (100mL liquid)
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1.Measure 1.6g Tripton
 +
2.Measure 1g Yeast Extract
 +
3.Measure 0.5g Nacl
 +
4.Add 100mL H2O
 +
5.autoclave(121℃ 20min)

Revision as of 03:00, 19 October 2016

Medium

LB medium (100 mL liquid) 1.Measure 1g Tripton 2.Measure 0.5g Yeast Extract 3.Measure 1g Nacl 4.Add 100mL H2O 5.autoclave(121℃ 20min)


2×YT medium (100mL liquid) 1.Measure 1.6g Tripton 2.Measure 1g Yeast Extract 3.Measure 0.5g Nacl 4.Add 100mL H2O 5.autoclave(121℃ 20min)