[2016-Attribution]

We are proud to say that all the ideas we worked on this year were proposed by undergraduate members of iGEM 2016 team Tokyo_Tech. We also want to thank all those advisors and instructors who helped us discuss these ideas and guided us through doing the experiments. The whole experimental data produced by our team comes from experiments all carried out by undergraduates.

The project "Snow White" was proposed by Ayako Tamaki.

[2016-member]

Performing all cloning (transformation, culture cells, mini prep)

- Kazuki Fujisawa, Yuya Netsu, Hiro Kondo, Koji Nishikawa, Yoshio Takata

Performing parts construction (restriction digest, ligation)

- Kazuki Fujisawa, Yuya Netsu, Hiro Kondo, Koji Nishikawa, Yoshio Takata

Performing Toxin assay

- mazEF system : Kentaro Akiyama, Kazuki Fujisawa

- yafNO system : Hiro Kondo, Yuya Netsu

Performing AHL assay

- Hazuki Hasegawa, Miki Nishimori

Performing AmiE assay

- Nae Fu

Performing Pheat assay

- Nae Fu

Performing Pcold assay

- Nae Fu

Modeling

- Shunya Akiyama, Ryuta Sasaki, Narumi Ogawa, Alex Llacsahuanga,

Wiki and slide design

- Ayako Tamaki, Ryuta Sasaki, Mizuki Tabata, Maoko Takenoshita, Jiawan Sun, Alex Llacsahuanga, Guo Xin, Hajime Fujita with help from other team members

Human Practices

- Hazuki Hasegawa, Nae Fu, Mako Kono, Nakahara Kengo, Nozomi Takeue with help from other team members

[2016-Contribution]

• General Support

  - Prof. Nakashima and Prof. Hayashi let us use their labs for all of our experiments and gave us advice on experimental skills.

  - Prof. Tagawa gave us advice on our project and helped us set up a forum.

  - Prof. Yamamura gave us advice on modeling.

  - Promega Corporation and Leave a Nest Co.,ltd gave us several kinds of restriction enzymes used in our experiments at no charge.

  - We'd like to thank all advisers and instructors who helped us through the discussion of these ideas and instructed us about experimental procedures.

• Instrumental Analysis Support

  - Biomaterial Analysis Center, Technical Department, checked the sequences of the plasmids which we asked.

• Gene Synthesis

  - We'd also like to thank MBL and IDT.

• Human Practice Support

  - Ochanomizu University Junior High School, Tokyo Metropolitan Nishi High School, Kichijo Girls’ School, Tokyo Gakugei University Senior High School, Kanagawa Prefectural Atsugi High School, and Koshigayakita High School warmly accepted our school visits.

  - Tastuya Ogawa, who is a science communicator at National Museum of Nature and Science, gave us important lessons about science communication.

  - Prof. Tsuge, Prof. Kakuta, and Dr. Jiro Nudeshima, who are working on The Tokyo Foundation, gave us lectures.

  - Prof. Nakasaki gave us great and helpful advice.

• Presentation Adviser

  - We'd like to thank all the people who came to check our presentations and gave us useful feedbacks:

  Professor:

  Yasunori Aizawa, Noriyuki Asakura, Hiroshi Iwasaki, Toshiaki Kamachi, Yasunori Tanji, Takeshi Hata, Takeshi Hirasawa, Junji Hirota, Shinji Masuda, Yuki Yamaguchi, Hideya Yuasa, Masaaki Wachi, Robert F. Whittier