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{{KAIT_Japan}} | {{KAIT_Japan}} | ||
<html> | <html> | ||
+ | <h1>Labonote</h1> | ||
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<br> | <br> | ||
8/17<br> | 8/17<br> | ||
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Ligation<br> | Ligation<br> | ||
PCR<br> | PCR<br> | ||
− | R0051 K592024 I14044 B0015 R0010 C0051 | + | R0051 K592024 I14044 B0015 R0010 C0051 <br> |
+ | <br> | ||
+ | 8/25<br> | ||
+ | Miniprep<br> | ||
+ | J04450<br> | ||
+ | <br> | ||
+ | 8/26<br> | ||
+ | Restriction enzyme treatment<br> | ||
+ | <br> | ||
+ | 8/29<br> | ||
+ | Purity a gel <br> | ||
+ | <br> | ||
+ | 8/30<br> | ||
+ | PCR | ||
+ | <br> | ||
+ | 8/31<br> | ||
+ | PCR<br> | ||
+ | 3A assembly<br> | ||
+ | K595024 + I14044<br> | ||
+ | <br> | ||
+ | 9/1<br> | ||
+ | PCR<br> | ||
+ | K595024 I14044 C0051 R0010 B0015 <br> | ||
+ | purity a gel <br> | ||
+ | preculture<br> | ||
+ | K595024 I14044 C0051 R0010 B0015 <br> | ||
+ | <br> | ||
+ | 9/2<br> | ||
+ | Miniprep<br> | ||
+ | K592024 I14044 C0051 R0010 B0015<br> | ||
+ | Pre-culture<br> | ||
+ | K592024 I14044 C0051 R010 B0015 J04450<br> | ||
+ | <br> | ||
+ | 9/3<br> | ||
+ | Miniprep<br> | ||
+ | K592024 I14044 C0051 R010 B0015 J04450<br> | ||
+ | <br> | ||
+ | 9/5<br> | ||
+ | Restriction enzyme treatment<br> | ||
+ | <br> | ||
+ | 9/6<br> | ||
+ | PCR<br> | ||
+ | Restriction enzyme treatment<br> | ||
+ | Pre-culture<br> | ||
+ | K592024 I14044 C0051 R010 B0015 J04450<br> | ||
+ | |||
+ | <br> | ||
+ | 9/7 | ||
+ | Miniprep<br> | ||
+ | K592024 I14044 C0051 R010 B0015 J04450<br> | ||
+ | Restriction enzyme treatment<br> | ||
+ | Pre-culture<br> | ||
+ | K592024 I14044 C0051 R010 B0015 J04450<br> | ||
+ | |||
+ | <br> | ||
+ | 9/8<br> | ||
+ | Miniprep<br> | ||
+ | K592024 I14044 C0051 R010 B0015 J04450<br> | ||
+ | |||
+ | <br> | ||
+ | 9/8<br> | ||
+ | PCR<br> | ||
+ | |||
+ | <br> | ||
+ | 9/12<br> | ||
+ | Gel purification<br> | ||
+ | |||
+ | |||
+ | |||
+ | <br> | ||
+ | 9/13<br> | ||
+ | Restriction enzyme treatment<br> | ||
+ | C0051 R010 B0015X B0015E J04450<br> | ||
+ | Pre-culture<br> | ||
+ | K592024 I14044 J04450<br> | ||
+ | Restriction enzyme treatment<br> | ||
+ | |||
+ | 9/14<br> | ||
+ | Restriction enzyme treatment<br> | ||
+ | Miniprep<br> | ||
+ | K592024 I14044 J04450<br> | ||
+ | |||
+ | <br> | ||
+ | 9/15<br> | ||
+ | Transformation<br> | ||
+ | Restriction enzyme treatment<br> | ||
+ | K592024 I14044 J04450<br> | ||
<br> | <br> | ||
9/16<br> | 9/16<br> | ||
− | + | PCR<br> | |
− | + | K592024 I14044 J04450<br> | |
+ | Miniprep<br> | ||
+ | Restriction emzyme fragmation<br> | ||
<br> | <br> | ||
9/19<br> | 9/19<br> | ||
− | + | Miniprep<br> | |
<br> | <br> | ||
9/21<br> | 9/21<br> | ||
− | + | Miniprep<br> | |
<br> | <br> | ||
9/22<br> | 9/22<br> | ||
− | + | Restriction emzyme fragmation<br> | |
− | + | Miniprep<br> | |
− | + | Purify a gel<br> | |
<br> | <br> | ||
9/23<br> | 9/23<br> | ||
− | + | Miniprep<br> | |
− | + | Purify a gel<br> | |
<br> | <br> | ||
Line 86: | Line 170: | ||
<br> | <br> | ||
9/26<br> | 9/26<br> | ||
− | + | Miniprep<br> | |
<br> | <br> | ||
9/27<br> | 9/27<br> | ||
− | + | Purify a gel<br> | |
− | + | Restriction emzyme fragmation<br> | |
<br> | <br> | ||
9/28<br> | 9/28<br> | ||
− | + | Restriction emzyme fragmation<br> | |
<br> | <br> | ||
9/30<br> | 9/30<br> | ||
− | + | Restriction emzyme fragmation<br> | |
− | + | Purify a gel<br> | |
<br> | <br> | ||
10/1<br> | 10/1<br> | ||
− | + | Ligation<br> | |
transformation<br> | transformation<br> | ||
− | + | Pre-culture<br> | |
− | + | Purification of antisensence<br> | |
<br> | <br> | ||
10/2<br> | 10/2<br> | ||
− | + | Pre-culture<br> | |
− | + | Miniprep<br> | |
− | + | Electrophoresis to confirm<br> | |
<br> | <br> | ||
Line 120: | Line 204: | ||
miniprep<br> | miniprep<br> | ||
PCR<br> | PCR<br> | ||
− | + | Pre-culture<br> | |
− | + | Purify a gel<br> | |
<br> | <br> | ||
10/4<br> | 10/4<br> | ||
− | + | Restriction emzyme fragmation<br> | |
− | + | Purify a gel<br> | |
− | + | Miniprep<br> | |
− | + | Electrophoresis to confirm<br> | |
<br> | <br> | ||
10/5<br> | 10/5<br> | ||
− | + | Ligation<br> | |
− | + | Transformation<br> | |
− | + | Purify a gel<br> | |
− | + | Restriction emzyme fragmation<br> | |
<br> | <br> | ||
10/6<br> | 10/6<br> | ||
KIB…miniprep<br> | KIB…miniprep<br> | ||
− | + | Electrophoresis to confirm<br> | |
− | + | Purify a gel<br> | |
<br> | <br> | ||
10/7<br> | 10/7<br> | ||
− | + | Restriction emzyme fragmation<br> | |
− | + | Ligation<br> | |
− | + | Transformation<br> | |
<br> | <br> | ||
10/8<br> | 10/8<br> | ||
− | + | Miniprep<br> | |
− | + | Purify a gel<br> | |
− | + | Restriction emzyme fragmation<br> | |
<br> | <br> | ||
10/9<br> | 10/9<br> | ||
− | + | Pre-culture<br> | |
− | + | Purify a gel<br> | |
<br> | <br> | ||
10/10<br> | 10/10<br> | ||
ligation<br> | ligation<br> | ||
− | + | Transformation<br> | |
<br> | <br> | ||
10/11<br> | 10/11<br> | ||
pre-culture<br> | pre-culture<br> | ||
− | + | Miniprep<br> | |
<br> | <br> | ||
10/12<br> | 10/12<br> | ||
− | + | Purify a gel<br> | |
<br> | <br> | ||
10/13<br> | 10/13<br> | ||
− | + | Electrophoresis to confirm<br> | |
<br> | <br> | ||
10/14<br> | 10/14<br> | ||
− | + | Restriction emzyme fragmation<br> | |
Line 192: | Line 276: | ||
10/16<br> | 10/16<br> | ||
electrophoresis to confirm<br> | electrophoresis to confirm<br> | ||
− | + | ||
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Revision as of 14:47, 19 October 2016
Labonote
8/17
restriction emzyme fragmation
K592024 B0015 C0051→Eco RI & SpeI
I14144 R100 B0015 →XbaI & PstI
EcoRI XbaI PstI →Promega
SpeI→Takara
8/18
transcription
R0051 K592024 I14044 B0015 R0010
C0051(psbic3)→E・coli-DH5X
8/18
miniprep
J04450
colony PCR
K592024 I14044 B0015 R0010 C0051
8/23
restriction emzyme fragmation
8/24
3A assembly
Ligation
PCR
R0051 K592024 I14044 B0015 R0010 C0051
8/25
Miniprep
J04450
8/26
Restriction enzyme treatment
8/29
Purity a gel
8/30
PCR
8/31
PCR
3A assembly
K595024 + I14044
9/1
PCR
K595024 I14044 C0051 R0010 B0015
purity a gel
preculture
K595024 I14044 C0051 R0010 B0015
9/2
Miniprep
K592024 I14044 C0051 R0010 B0015
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
9/3
Miniprep
K592024 I14044 C0051 R010 B0015 J04450
9/5
Restriction enzyme treatment
9/6
PCR
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
9/7 Miniprep
K592024 I14044 C0051 R010 B0015 J04450
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
9/8
Miniprep
K592024 I14044 C0051 R010 B0015 J04450
9/8
PCR
9/12
Gel purification
9/13
Restriction enzyme treatment
C0051 R010 B0015X B0015E J04450
Pre-culture
K592024 I14044 J04450
Restriction enzyme treatment
9/14
Restriction enzyme treatment
Miniprep
K592024 I14044 J04450
9/15
Transformation
Restriction enzyme treatment
K592024 I14044 J04450
9/16
PCR
K592024 I14044 J04450
Miniprep
Restriction emzyme fragmation
9/19
Miniprep
9/21
Miniprep
9/22
Restriction emzyme fragmation
Miniprep
Purify a gel
9/23
Miniprep
Purify a gel
9/24
restriction emzyme fragmation
9/25
purify a gel
ligation
transformation
9/26
Miniprep
9/27
Purify a gel
Restriction emzyme fragmation
9/28
Restriction emzyme fragmation
9/30
Restriction emzyme fragmation
Purify a gel
10/1
Ligation
transformation
Pre-culture
Purification of antisensence
10/2
Pre-culture
Miniprep
Electrophoresis to confirm
10/3
miniprep
PCR
Pre-culture
Purify a gel
10/4
Restriction emzyme fragmation
Purify a gel
Miniprep
Electrophoresis to confirm
10/5
Ligation
Transformation
Purify a gel
Restriction emzyme fragmation
10/6
KIB…miniprep
Electrophoresis to confirm
Purify a gel
10/7
Restriction emzyme fragmation
Ligation
Transformation
10/8
Miniprep
Purify a gel
Restriction emzyme fragmation
10/9
Pre-culture
Purify a gel
10/10
ligation
Transformation
10/11
pre-culture
Miniprep
10/12
Purify a gel
10/13
Electrophoresis to confirm
10/14
Restriction emzyme fragmation
10/15
electrophoresis to confirm
restriction emzyme fragmation
10/16
electrophoresis to confirm