Difference between revisions of "Team:KAIT Japan/Labnote"

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{{KAIT_Japan}}
 
{{KAIT_Japan}}
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<h1>Labonote</h1>
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}<h1>Labonote</h1>
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10/16<br>
 
10/16<br>
 
electrophoresis to confirm<br>
 
electrophoresis to confirm<br>
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<br>
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Revision as of 14:56, 19 October 2016

Labonote


8/17
restriction emzyme fragmation
K592024 B0015 C0051→Eco RI & SpeI
I14144 R100 B0015 →XbaI & PstI
EcoRI XbaI PstI →Promega
SpeI→Takara

8/18
transcription
R0051 K592024 I14044 B0015 R0010
C0051(psbic3)→E・coli-DH5X

8/18
miniprep
J04450
colony PCR
K592024 I14044 B0015 R0010 C0051

8/23
restriction emzyme fragmation

8/24
3A assembly
Ligation
PCR
R0051 K592024 I14044 B0015 R0010 C0051

8/25
Miniprep
J04450

8/26
Restriction enzyme treatment

8/29
Purity a gel

8/30
PCR
8/31
PCR
3A assembly
K595024 + I14044

9/1
PCR
K595024 I14044 C0051 R0010 B0015
purity a gel
preculture
K595024 I14044 C0051 R0010 B0015

9/2
Miniprep
K592024 I14044 C0051 R0010 B0015
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450

9/3
Miniprep
K592024 I14044 C0051 R010 B0015 J04450

9/5
Restriction enzyme treatment

9/6
PCR
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450

9/7 Miniprep
K592024 I14044 C0051 R010 B0015 J04450
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450

9/8
Miniprep
K592024 I14044 C0051 R010 B0015 J04450

9/8
PCR

9/12
Gel purification

9/13
Restriction enzyme treatment
C0051 R010 B0015X B0015E J04450
Pre-culture
K592024 I14044 J04450
Restriction enzyme treatment
9/14
Restriction enzyme treatment
Miniprep
K592024 I14044 J04450

9/15
Transformation
Restriction enzyme treatment
K592024 I14044 J04450

9/16
PCR
K592024 I14044 J04450
Miniprep
Restriction emzyme fragmation

9/19
Miniprep

9/21
Miniprep

9/22
Restriction emzyme fragmation
Miniprep
Purify a gel

9/23
Miniprep
Purify a gel

9/24
restriction emzyme fragmation

9/25
purify a gel
ligation
transformation

9/26
Miniprep

9/27
Purify a gel
Restriction emzyme fragmation

9/28
Restriction emzyme fragmation

9/30
Restriction emzyme fragmation
Purify a gel

10/1
Ligation
transformation
Pre-culture
Purification of antisensence

10/2
Pre-culture
Miniprep
Electrophoresis to confirm

10/3
miniprep
PCR
Pre-culture
Purify a gel

10/4
Restriction emzyme fragmation
Purify a gel
Miniprep
Electrophoresis to confirm

10/5
Ligation
Transformation
Purify a gel
Restriction emzyme fragmation

10/6
KIB…miniprep
Electrophoresis to confirm
Purify a gel

10/7
Restriction emzyme fragmation
Ligation
Transformation

10/8
Miniprep
Purify a gel
Restriction emzyme fragmation

10/9
Pre-culture
Purify a gel

10/10
ligation
Transformation

10/11
pre-culture
Miniprep

10/12
Purify a gel

10/13
Electrophoresis to confirm

10/14
Restriction emzyme fragmation

10/15
electrophoresis to confirm
restriction emzyme fragmation

10/16
electrophoresis to confirm