Difference between revisions of "Team:KAIT Japan/Labnote"
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<h1>Labonote</h1> | <h1>Labonote</h1> | ||
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8/17<br> | 8/17<br> | ||
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10/10<br> | 10/10<br> | ||
| − | + | Ligation<br> | |
Transformation<br> | Transformation<br> | ||
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10/15<br> | 10/15<br> | ||
| − | + | Electrophoresis to confirm<br> | |
| − | + | Restriction emzyme fragmation<br> | |
<br> | <br> | ||
10/16<br> | 10/16<br> | ||
| − | + | Electrophoresis to confirm<br> | |
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Revision as of 15:27, 19 October 2016
Labonote
8/17
restriction emzyme fragmation
K592024 B0015 C0051→Eco RI & SpeI
I14144 R100 B0015 →XbaI & PstI
EcoRI XbaI PstI →Promega
SpeI→Takara
8/18
transcription
R0051 K592024 I14044 B0015 R0010
C0051(psbic3)→E・coli-DH5X
8/18
miniprep
J04450
colony PCR
K592024 I14044 B0015 R0010 C0051
8/23
restriction emzyme fragmation
8/24
3A assembly
Ligation
PCR
R0051 K592024 I14044 B0015 R0010 C0051
8/25
Miniprep
J04450
8/26
Restriction enzyme treatment
8/29
Purity a gel
8/30
PCR
8/31
PCR
3A assembly
K595024 + I14044
9/1
PCR
K595024 I14044 C0051 R0010 B0015
purity a gel
preculture
K595024 I14044 C0051 R0010 B0015
9/2
Miniprep
K592024 I14044 C0051 R0010 B0015
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
9/3
Miniprep
K592024 I14044 C0051 R010 B0015 J04450
9/5
Restriction enzyme treatment
9/6
PCR
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
9/7 Miniprep
K592024 I14044 C0051 R010 B0015 J04450
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
9/8
Miniprep
K592024 I14044 C0051 R010 B0015 J04450
9/8
PCR
9/12
Gel purification
9/13
Restriction enzyme treatment
C0051 R010 B0015X B0015E J04450
Pre-culture
K592024 I14044 J04450
Restriction enzyme treatment
9/14
Restriction enzyme treatment
Miniprep
K592024 I14044 J04450
9/15
Transformation
Restriction enzyme treatment
K592024 I14044 J04450
9/16
PCR
K592024 I14044 J04450
Miniprep
Restriction emzyme fragmation
9/19
Miniprep
9/21
Miniprep
9/22
Restriction emzyme fragmation
Miniprep
Purify a gel
9/23
Miniprep
Purify a gel
9/24
restriction emzyme fragmation
9/25
purify a gel
ligation
transformation
9/26
Miniprep
9/27
Purify a gel
Restriction emzyme fragmation
9/28
Restriction emzyme fragmation
9/30
Restriction emzyme fragmation
Purify a gel
10/1
Ligation
transformation
Pre-culture
Purification of antisensence
10/2
Pre-culture
Miniprep
Electrophoresis to confirm
10/3
miniprep
PCR
Pre-culture
Purify a gel
10/4
Restriction emzyme fragmation
Purify a gel
Miniprep
Electrophoresis to confirm
10/5
Ligation
Transformation
Purify a gel
Restriction emzyme fragmation
10/6
KIB…miniprep
Electrophoresis to confirm
Purify a gel
10/7
Restriction emzyme fragmation
Ligation
Transformation
10/8
Miniprep
Purify a gel
Restriction emzyme fragmation
10/9
Pre-culture
Purify a gel
10/10
Ligation
Transformation
10/11
pre-culture
Miniprep
10/12
Purify a gel
10/13
Electrophoresis to confirm
10/14
Restriction emzyme fragmation
10/15
Electrophoresis to confirm
Restriction emzyme fragmation
10/16
Electrophoresis to confirm
