Difference between revisions of "Team:KAIT Japan/Labnote"

Line 14: Line 14:
  
 
<br>
 
<br>
−
8/18<br>
+
<dt>8/18<br>
 
transcription<br>
 
transcription<br>
 
R0051 K592024 I14044 B0015 R0010<br>
 
R0051 K592024 I14044 B0015 R0010<br>
Line 20: Line 20:
  
 
<br>
 
<br>
−
8/18<br>
+
<dt>8/18<br>
 
miniprep<br>
 
miniprep<br>
 
J04450<br>
 
J04450<br>
Line 27: Line 27:
  
 
<br>
 
<br>
−
8/23<br>
+
<dt>8/23<br>
 
restriction emzyme fragmation<br>
 
restriction emzyme fragmation<br>
  
 
<br>
 
<br>
−
8/24<br>
+
<dt>8/24<br>
 
3A assembly<br>
 
3A assembly<br>
 
Ligation<br>
 
Ligation<br>
Line 38: Line 38:
  
 
<br>
 
<br>
−
8/25<br>
+
<dt>8/25<br>
 
Miniprep<br>
 
Miniprep<br>
 
J04450<br>
 
J04450<br>
Line 47: Line 47:
  
 
<br>
 
<br>
−
8/29<br>
+
<dt>8/29<br>
 
Purity a gel <br>
 
Purity a gel <br>
  
 
<br>
 
<br>
−
8/30<br>
+
<dt>8/30<br>
 
PCR
 
PCR
  
 
<br>
 
<br>
−
8/31<br>
+
<dt>8/31<br>
 
PCR<br>
 
PCR<br>
 
3A assembly<br>
 
3A assembly<br>
Line 61: Line 61:
  
 
<br>
 
<br>
−
9/1<br>
+
<dt>9/1<br>
 
PCR<br>
 
PCR<br>
 
K595024 I14044 C0051 R0010 B0015 <br>
 
K595024 I14044 C0051 R0010 B0015 <br>
Line 76: Line 76:
  
 
<br>
 
<br>
−
9/3<br>
+
<dt>9/3<br>
 
Miniprep<br>
 
Miniprep<br>
 
K592024 I14044 C0051 R010 B0015 J04450<br>
 
K592024 I14044 C0051 R010 B0015 J04450<br>
  
 
<br>
 
<br>
−
9/5<br>
+
<dt>9/5<br>
 
Restriction enzyme treatment<br>
 
Restriction enzyme treatment<br>
  
 
<br>
 
<br>
−
9/6<br>
+
<dt>9/6<br>
 
PCR<br>
 
PCR<br>
 
Restriction enzyme treatment<br>
 
Restriction enzyme treatment<br>
Line 92: Line 92:
  
 
<br>
 
<br>
−
9/7
+
<dt>9/7
 
Miniprep<br>
 
Miniprep<br>
 
K592024 I14044 C0051 R010 B0015 J04450<br>
 
K592024 I14044 C0051 R010 B0015 J04450<br>
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<br>
 
<br>
−
9/8<br>
+
<dt>9/8<br>
 
Miniprep<br>
 
Miniprep<br>
 
K592024 I14044 C0051 R010 B0015 J04450<br>
 
K592024 I14044 C0051 R010 B0015 J04450<br>
  
 
<br>
 
<br>
−
9/8<br>
+
<dt>9/8<br>
 
PCR<br>
 
PCR<br>
  
 
<br>
 
<br>
−
9/12<br>
+
<dt>9/12<br>
 
Gel purification<br>
 
Gel purification<br>
  
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<br>
 
<br>
−
9/13<br>
+
<dt>9/13<br>
 
Restriction enzyme treatment<br>
 
Restriction enzyme treatment<br>
 
C0051 R010 B0015X B0015E J04450<br>
 
C0051 R010 B0015X B0015E J04450<br>
Line 122: Line 122:
 
Restriction enzyme treatment<br>
 
Restriction enzyme treatment<br>
  
−
9/14<br>
+
<br>
 +
<dt>9/14<br>
 
Restriction enzyme treatment<br>
 
Restriction enzyme treatment<br>
 
Miniprep<br>
 
Miniprep<br>
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<br>
 
<br>
−
9/15<br>
+
<dt>/15<br>
 
Transformation<br>
 
Transformation<br>
 
Restriction enzyme treatment<br>
 
Restriction enzyme treatment<br>
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<br>
 
<br>
−
9/16<br>
+
<dt>9/16<br>
 
PCR<br>
 
PCR<br>
 
K592024 I14044 J04450<br>
 
K592024 I14044 J04450<br>
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<br>
 
<br>
−
9/19<br>
+
<dt>9/19<br>
 
Miniprep<br>
 
Miniprep<br>
  
 
<br>
 
<br>
−
9/21<br>
+
<dt>9/21<br>
 
Miniprep<br>
 
Miniprep<br>
  
 
<br>
 
<br>
−
9/22<br>
+
<dt>9/22<br>
 
Restriction emzyme fragmation<br>
 
Restriction emzyme fragmation<br>
 
Miniprep<br>
 
Miniprep<br>
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<br>
 
<br>
−
9/23<br>
+
<dt>9/23<br>
 
Miniprep<br>
 
Miniprep<br>
 
Purify a gel<br>
 
Purify a gel<br>
  
 
<br>
 
<br>
−
9/24<br>
+
<dt>9/24<br>
 
restriction emzyme fragmation<br>
 
restriction emzyme fragmation<br>
  
 
<br>
 
<br>
−
9/25<br>
+
<dt>9/25<br>
 
purify a gel<br>
 
purify a gel<br>
 
ligation<br>
 
ligation<br>
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<br>
 
<br>
−
9/26<br>
+
<dt>9/26<br>
 
Miniprep<br>
 
Miniprep<br>
  
 
<br>
 
<br>
−
9/27<br>
+
<dt>9/27<br>
 
Purify a gel<br>
 
Purify a gel<br>
 
Restriction emzyme fragmation<br>
 
Restriction emzyme fragmation<br>
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<br>
 
<br>
−
9/30<br>
+
<dt>9/30<br>
 
Restriction emzyme fragmation<br>
 
Restriction emzyme fragmation<br>
 
Purify a gel<br>
 
Purify a gel<br>
  
 
<br>
 
<br>
−
10/1<br>
+
<dt>10/1<br>
 
Ligation<br>
 
Ligation<br>
 
transformation<br>
 
transformation<br>
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<br>
 
<br>
−
10/2<br>
+
<dt>10/2<br>
 
Pre-culture<br>
 
Pre-culture<br>
 
Miniprep<br>
 
Miniprep<br>
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<br>
 
<br>
−
10/3<br>
+
<dt>10/3<br>
 
miniprep<br>
 
miniprep<br>
 
PCR<br>
 
PCR<br>
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<br>
 
<br>
−
10/4<br>
+
<dt>10/4<br>
 
Restriction emzyme fragmation<br>
 
Restriction emzyme fragmation<br>
 
Purify a gel<br>
 
Purify a gel<br>
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<br>
 
<br>
−
10/5<br>
+
<dt>10/5<br>
 
Ligation<br>
 
Ligation<br>
 
Transformation<br>
 
Transformation<br>
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<br>
 
<br>
−
10/6<br>
+
<dt>10/6<br>
 
KIB…miniprep<br>
 
KIB…miniprep<br>
 
Electrophoresis to confirm<br>
 
Electrophoresis to confirm<br>
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<br>
 
<br>
−
10/7<br>
+
<dt>10/7<br>
 
Restriction emzyme fragmation<br>
 
Restriction emzyme fragmation<br>
 
Ligation<br>
 
Ligation<br>
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<br>
 
<br>
−
10/8<br>
+
<dt>10/8<br>
 
Miniprep<br>
 
Miniprep<br>
 
Purify a gel<br>
 
Purify a gel<br>
Line 242: Line 243:
  
 
<br>
 
<br>
−
10/9<br>
+
<dt>10/9<br>
 
Pre-culture<br>
 
Pre-culture<br>
 
Purify a gel<br>
 
Purify a gel<br>
  
 
<br>
 
<br>
−
10/10<br>
+
<dt>0/10<br>
 
Ligation<br>
 
Ligation<br>
 
Transformation<br>
 
Transformation<br>
  
 
<br>
 
<br>
−
10/11<br>
+
<dt>10/11<br>
 
pre-culture<br>
 
pre-culture<br>
 
Miniprep<br>
 
Miniprep<br>
  
 
<br>
 
<br>
−
10/12<br>
+
<dt>10/12<br>
 
Purify a gel<br>
 
Purify a gel<br>
  
 
<br>
 
<br>
−
10/13<br>
+
<dt>10/13<br>
 
Electrophoresis to confirm<br>
 
Electrophoresis to confirm<br>
  
 
<br>
 
<br>
−
10/14<br>
+
<dt>10/14<br>
 
Restriction emzyme fragmation<br>
 
Restriction emzyme fragmation<br>
  
  
 
<br>
 
<br>
−
10/15<br>
+
<dt>10/15<br>
 
Electrophoresis to confirm<br>
 
Electrophoresis to confirm<br>
 
Restriction emzyme fragmation<br>
 
Restriction emzyme fragmation<br>
Line 276: Line 277:
  
 
<br>
 
<br>
−
10/16<br>
+
<dt>10/16<br>
 
Electrophoresis to confirm<br>
 
Electrophoresis to confirm<br>
 
<br>
 
<br>

Revision as of 15:49, 19 October 2016

Labonote

8/17
restriction emzyme fragmation
K592024 B0015 C0051→Eco RI & SpeI
I14144 R100 B0015 →XbaI & PstI
EcoRI XbaI PstI →Promega
SpeI→Takara

8/18
transcription
R0051 K592024 I14044 B0015 R0010
C0051(psbic3)→E・coli-DH5X

8/18
miniprep
J04450
colony PCR
K592024 I14044 B0015 R0010 C0051

8/23
restriction emzyme fragmation

8/24
3A assembly
Ligation
PCR
R0051 K592024 I14044 B0015 R0010 C0051

8/25
Miniprep
J04450

8/26
Restriction enzyme treatment

8/29
Purity a gel

8/30
PCR
8/31
PCR
3A assembly
K595024 + I14044

9/1
PCR
K595024 I14044 C0051 R0010 B0015
purity a gel
preculture
K595024 I14044 C0051 R0010 B0015

9/2
Miniprep
K592024 I14044 C0051 R0010 B0015
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450

9/3
Miniprep
K592024 I14044 C0051 R010 B0015 J04450

9/5
Restriction enzyme treatment

9/6
PCR
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450

9/7 Miniprep
K592024 I14044 C0051 R010 B0015 J04450
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450

9/8
Miniprep
K592024 I14044 C0051 R010 B0015 J04450

9/8
PCR

9/12
Gel purification

9/13
Restriction enzyme treatment
C0051 R010 B0015X B0015E J04450
Pre-culture
K592024 I14044 J04450
Restriction enzyme treatment

9/14
Restriction enzyme treatment
Miniprep
K592024 I14044 J04450

/15
Transformation
Restriction enzyme treatment
K592024 I14044 J04450

9/16
PCR
K592024 I14044 J04450
Miniprep
Restriction emzyme fragmation

9/19
Miniprep

9/21
Miniprep

9/22
Restriction emzyme fragmation
Miniprep
Purify a gel

9/23
Miniprep
Purify a gel

9/24
restriction emzyme fragmation

9/25
purify a gel
ligation
transformation

9/26
Miniprep

9/27
Purify a gel
Restriction emzyme fragmation

9/28
Restriction emzyme fragmation

9/30
Restriction emzyme fragmation
Purify a gel

10/1
Ligation
transformation
Pre-culture
Purification of antisensence

10/2
Pre-culture
Miniprep
Electrophoresis to confirm

10/3
miniprep
PCR
Pre-culture
Purify a gel

10/4
Restriction emzyme fragmation
Purify a gel
Miniprep
Electrophoresis to confirm

10/5
Ligation
Transformation
Purify a gel
Restriction emzyme fragmation

10/6
KIB…miniprep
Electrophoresis to confirm
Purify a gel

10/7
Restriction emzyme fragmation
Ligation
Transformation

10/8
Miniprep
Purify a gel
Restriction emzyme fragmation

10/9
Pre-culture
Purify a gel

0/10
Ligation
Transformation

10/11
pre-culture
Miniprep

10/12
Purify a gel

10/13
Electrophoresis to confirm

10/14
Restriction emzyme fragmation

10/15
Electrophoresis to confirm
Restriction emzyme fragmation

10/16
Electrophoresis to confirm