Difference between revisions of "Team:KAIT Japan/Labnote"

Line 15: Line 15:
 
<br>
 
<br>
 
<dt>8/18<br>
 
<dt>8/18<br>
−
transcription<br>
+
<dd>transcription<br>
 
R0051 K592024 I14044 B0015 R0010<br>
 
R0051 K592024 I14044 B0015 R0010<br>
 
C0051(psbic3)→E・coli-DH5X<br>
 
C0051(psbic3)→E・coli-DH5X<br>
Line 21: Line 21:
 
<br>
 
<br>
 
<dt>8/18<br>
 
<dt>8/18<br>
−
miniprep<br>
+
<dd>miniprep<br>
 
J04450<br>
 
J04450<br>
 
colony PCR<br>
 
colony PCR<br>
Line 28: Line 28:
 
<br>
 
<br>
 
<dt>8/23<br>
 
<dt>8/23<br>
−
restriction emzyme fragmation<br>
+
<dd>restriction emzyme fragmation<br>
  
 
<br>
 
<br>
 
<dt>8/24<br>
 
<dt>8/24<br>
−
3A assembly<br>
+
<dd>3A assembly<br>
 
Ligation<br>
 
Ligation<br>
 
PCR<br>
 
PCR<br>
Line 39: Line 39:
 
<br>
 
<br>
 
<dt>8/25<br>
 
<dt>8/25<br>
−
Miniprep<br>
+
<dd>Miniprep<br>
 
J04450<br>
 
J04450<br>
  
 
<br>
 
<br>
−
8/26<br>
+
<dt>8/26<br>
−
Restriction enzyme treatment<br>
+
<dd>Restriction enzyme treatment<br>
  
 
<br>
 
<br>
 
<dt>8/29<br>
 
<dt>8/29<br>
−
Purity a gel <br>
+
<dd>Purity a gel <br>
  
 
<br>
 
<br>
 
<dt>8/30<br>
 
<dt>8/30<br>
−
PCR
+
<dd>PCR
  
 
<br>
 
<br>
 
<dt>8/31<br>
 
<dt>8/31<br>
−
PCR<br>
+
<dd>PCR<br>
 
3A assembly<br>
 
3A assembly<br>
 
K595024 + I14044<br>
 
K595024 + I14044<br>
Line 62: Line 62:
 
<br>
 
<br>
 
<dt>9/1<br>
 
<dt>9/1<br>
−
PCR<br>
+
<dd>PCR<br>
 
K595024 I14044 C0051 R0010 B0015 <br>
 
K595024 I14044 C0051 R0010 B0015 <br>
 
purity a gel <br>
 
purity a gel <br>
Line 69: Line 69:
  
 
<br>
 
<br>
−
9/2<br>
+
<dt>9/2<br>
−
Miniprep<br>
+
<dd>Miniprep<br>
 
K592024 I14044 C0051 R0010 B0015<br>
 
K592024 I14044 C0051 R0010 B0015<br>
 
Pre-culture<br>
 
Pre-culture<br>
Line 77: Line 77:
 
<br>
 
<br>
 
<dt>9/3<br>
 
<dt>9/3<br>
−
Miniprep<br>
+
<dd>Miniprep<br>
 
K592024 I14044 C0051 R010 B0015 J04450<br>
 
K592024 I14044 C0051 R010 B0015 J04450<br>
  
 
<br>
 
<br>
 
<dt>9/5<br>
 
<dt>9/5<br>
−
Restriction enzyme treatment<br>
+
<dd>Restriction enzyme treatment<br>
  
 
<br>
 
<br>
 
<dt>9/6<br>
 
<dt>9/6<br>
−
PCR<br>
+
<dd>PCR<br>
 
Restriction enzyme treatment<br>
 
Restriction enzyme treatment<br>
 
Pre-culture<br>
 
Pre-culture<br>
Line 93: Line 93:
 
<br>
 
<br>
 
<dt>9/7
 
<dt>9/7
−
Miniprep<br>
+
<dd>Miniprep<br>
 
K592024 I14044 C0051 R010 B0015 J04450<br>
 
K592024 I14044 C0051 R010 B0015 J04450<br>
 
Restriction enzyme treatment<br>
 
Restriction enzyme treatment<br>
Line 101: Line 101:
 
<br>
 
<br>
 
<dt>9/8<br>
 
<dt>9/8<br>
−
Miniprep<br>
+
<dd>Miniprep<br>
 
K592024 I14044 C0051 R010 B0015 J04450<br>
 
K592024 I14044 C0051 R010 B0015 J04450<br>
  
 
<br>
 
<br>
 
<dt>9/8<br>
 
<dt>9/8<br>
−
PCR<br>
+
<dd>PCR<br>
  
 
<br>
 
<br>
 
<dt>9/12<br>
 
<dt>9/12<br>
−
Gel purification<br>
+
<dd>Gel purification<br>
−
 
+
−
 
+
  
 
<br>
 
<br>
 
<dt>9/13<br>
 
<dt>9/13<br>
−
Restriction enzyme treatment<br>
+
<dd>Restriction enzyme treatment<br>
 
C0051 R010 B0015X B0015E J04450<br>
 
C0051 R010 B0015X B0015E J04450<br>
 
Pre-culture<br>
 
Pre-culture<br>
Line 124: Line 122:
 
<br>
 
<br>
 
<dt>9/14<br>
 
<dt>9/14<br>
−
Restriction enzyme treatment<br>
+
<dd>Restriction enzyme treatment<br>
 
Miniprep<br>
 
Miniprep<br>
 
K592024 I14044 J04450<br>
 
K592024 I14044 J04450<br>
Line 130: Line 128:
 
<br>
 
<br>
 
<dt>/15<br>
 
<dt>/15<br>
−
Transformation<br>
+
<dd>Transformation<br>
 
Restriction enzyme treatment<br>
 
Restriction enzyme treatment<br>
 
K592024 I14044 J04450<br>
 
K592024 I14044 J04450<br>
Line 136: Line 134:
 
<br>
 
<br>
 
<dt>9/16<br>
 
<dt>9/16<br>
−
PCR<br>
+
<dd>PCR<br>
 
K592024 I14044 J04450<br>
 
K592024 I14044 J04450<br>
 
Miniprep<br>
 
Miniprep<br>
Line 144: Line 142:
 
<br>
 
<br>
 
<dt>9/19<br>
 
<dt>9/19<br>
−
Miniprep<br>
+
<dd>Miniprep<br>
  
 
<br>
 
<br>
 
<dt>9/21<br>
 
<dt>9/21<br>
−
Miniprep<br>
+
<dd>Miniprep<br>
  
 
<br>
 
<br>
 
<dt>9/22<br>
 
<dt>9/22<br>
−
Restriction emzyme fragmation<br>
+
<dd>Restriction emzyme fragmation<br>
 
Miniprep<br>
 
Miniprep<br>
 
Purify a gel<br>
 
Purify a gel<br>
Line 158: Line 156:
 
<br>
 
<br>
 
<dt>9/23<br>
 
<dt>9/23<br>
−
Miniprep<br>
+
<dd>Miniprep<br>
 
Purify a gel<br>
 
Purify a gel<br>
  
 
<br>
 
<br>
 
<dt>9/24<br>
 
<dt>9/24<br>
−
restriction emzyme fragmation<br>
+
<dd>Restriction emzyme fragmation<br>
  
 
<br>
 
<br>
 
<dt>9/25<br>
 
<dt>9/25<br>
−
purify a gel<br>
+
<dd>Purify a gel<br>
−
ligation<br>
+
Ligation<br>
−
transformation<br>
+
Transformation<br>
  
 
<br>
 
<br>
 
<dt>9/26<br>
 
<dt>9/26<br>
−
Miniprep<br>
+
<dd>Miniprep<br>
  
 
<br>
 
<br>
 
<dt>9/27<br>
 
<dt>9/27<br>
−
Purify a gel<br>
+
<dd>Purify a gel<br>
 
Restriction emzyme fragmation<br>
 
Restriction emzyme fragmation<br>
  
  
 
<br>
 
<br>
−
9/28<br>
+
<dt>9/28<br>
−
Restriction emzyme fragmation<br>
+
<dd>Restriction emzyme fragmation<br>
  
 
<br>
 
<br>
 
<dt>9/30<br>
 
<dt>9/30<br>
−
Restriction emzyme fragmation<br>
+
<dd>Restriction emzyme fragmation<br>
 
Purify a gel<br>
 
Purify a gel<br>
  
 
<br>
 
<br>
 
<dt>10/1<br>
 
<dt>10/1<br>
−
Ligation<br>
+
<dd>Ligation<br>
 
transformation<br>
 
transformation<br>
 
Pre-culture<br>
 
Pre-culture<br>
Line 199: Line 197:
 
<br>
 
<br>
 
<dt>10/2<br>
 
<dt>10/2<br>
−
Pre-culture<br>
+
<dd>Pre-culture<br>
 
Miniprep<br>
 
Miniprep<br>
 
Electrophoresis to confirm<br>
 
Electrophoresis to confirm<br>
Line 205: Line 203:
 
<br>
 
<br>
 
<dt>10/3<br>
 
<dt>10/3<br>
−
miniprep<br>
+
<dd>miniprep<br>
 
PCR<br>
 
PCR<br>
 
Pre-culture<br>
 
Pre-culture<br>
Line 212: Line 210:
 
<br>
 
<br>
 
<dt>10/4<br>
 
<dt>10/4<br>
−
Restriction emzyme fragmation<br>
+
<dd>Restriction emzyme fragmation<br>
 
Purify a gel<br>
 
Purify a gel<br>
 
Miniprep<br>
 
Miniprep<br>
Line 219: Line 217:
 
<br>
 
<br>
 
<dt>10/5<br>
 
<dt>10/5<br>
−
Ligation<br>
+
<dd>Ligation<br>
 
Transformation<br>
 
Transformation<br>
 
Purify a gel<br>
 
Purify a gel<br>
Line 226: Line 224:
 
<br>
 
<br>
 
<dt>10/6<br>
 
<dt>10/6<br>
−
KIB…miniprep<br>
+
<dd>KIB…miniprep<br>
 
Electrophoresis to confirm<br>
 
Electrophoresis to confirm<br>
 
Purify a gel<br>
 
Purify a gel<br>
Line 232: Line 230:
 
<br>
 
<br>
 
<dt>10/7<br>
 
<dt>10/7<br>
−
Restriction emzyme fragmation<br>
+
<dd>Restriction emzyme fragmation<br>
 
Ligation<br>
 
Ligation<br>
 
Transformation<br>
 
Transformation<br>
Line 238: Line 236:
 
<br>
 
<br>
 
<dt>10/8<br>
 
<dt>10/8<br>
−
Miniprep<br>
+
<dd>Miniprep<br>
 
Purify a gel<br>
 
Purify a gel<br>
 
Restriction emzyme fragmation<br>
 
Restriction emzyme fragmation<br>
Line 244: Line 242:
 
<br>
 
<br>
 
<dt>10/9<br>
 
<dt>10/9<br>
−
Pre-culture<br>
+
<dd>Pre-culture<br>
 
Purify a gel<br>
 
Purify a gel<br>
  
 
<br>
 
<br>
 
<dt>0/10<br>
 
<dt>0/10<br>
−
Ligation<br>
+
<dd>Ligation<br>
 
Transformation<br>
 
Transformation<br>
  
 
<br>
 
<br>
 
<dt>10/11<br>
 
<dt>10/11<br>
−
pre-culture<br>
+
<dd>pre-culture<br>
 
Miniprep<br>
 
Miniprep<br>
  
 
<br>
 
<br>
 
<dt>10/12<br>
 
<dt>10/12<br>
−
Purify a gel<br>
+
<dd>Purify a gel<br>
  
 
<br>
 
<br>
 
<dt>10/13<br>
 
<dt>10/13<br>
−
Electrophoresis to confirm<br>
+
<dd>Electrophoresis to confirm<br>
  
 
<br>
 
<br>
 
<dt>10/14<br>
 
<dt>10/14<br>
−
Restriction emzyme fragmation<br>
+
<dd>Restriction emzyme fragmation<br>
  
  
 
<br>
 
<br>
 
<dt>10/15<br>
 
<dt>10/15<br>
−
Electrophoresis to confirm<br>
+
<dd>Electrophoresis to confirm<br>
 
Restriction emzyme fragmation<br>
 
Restriction emzyme fragmation<br>
  
Line 278: Line 276:
 
<br>
 
<br>
 
<dt>10/16<br>
 
<dt>10/16<br>
−
Electrophoresis to confirm<br>
+
<dd>Electrophoresis to confirm<br>
 
<br>
 
<br>
  

Revision as of 15:53, 19 October 2016

Labonote

8/17
restriction emzyme fragmation
K592024 B0015 C0051→Eco RI & SpeI
I14144 R100 B0015 →XbaI & PstI
EcoRI XbaI PstI →Promega
SpeI→Takara

8/18
transcription
R0051 K592024 I14044 B0015 R0010
C0051(psbic3)→E・coli-DH5X

8/18
miniprep
J04450
colony PCR
K592024 I14044 B0015 R0010 C0051

8/23
restriction emzyme fragmation

8/24
3A assembly
Ligation
PCR
R0051 K592024 I14044 B0015 R0010 C0051

8/25
Miniprep
J04450

8/26
Restriction enzyme treatment

8/29
Purity a gel

8/30
PCR
8/31
PCR
3A assembly
K595024 + I14044

9/1
PCR
K595024 I14044 C0051 R0010 B0015
purity a gel
preculture
K595024 I14044 C0051 R0010 B0015

9/2
Miniprep
K592024 I14044 C0051 R0010 B0015
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450

9/3
Miniprep
K592024 I14044 C0051 R010 B0015 J04450

9/5
Restriction enzyme treatment

9/6
PCR
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450

9/7
Miniprep
K592024 I14044 C0051 R010 B0015 J04450
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450

9/8
Miniprep
K592024 I14044 C0051 R010 B0015 J04450

9/8
PCR

9/12
Gel purification

9/13
Restriction enzyme treatment
C0051 R010 B0015X B0015E J04450
Pre-culture
K592024 I14044 J04450
Restriction enzyme treatment

9/14
Restriction enzyme treatment
Miniprep
K592024 I14044 J04450

/15
Transformation
Restriction enzyme treatment
K592024 I14044 J04450

9/16
PCR
K592024 I14044 J04450
Miniprep
Restriction emzyme fragmation

9/19
Miniprep

9/21
Miniprep

9/22
Restriction emzyme fragmation
Miniprep
Purify a gel

9/23
Miniprep
Purify a gel

9/24
Restriction emzyme fragmation

9/25
Purify a gel
Ligation
Transformation

9/26
Miniprep

9/27
Purify a gel
Restriction emzyme fragmation

9/28
Restriction emzyme fragmation

9/30
Restriction emzyme fragmation
Purify a gel

10/1
Ligation
transformation
Pre-culture
Purification of antisensence

10/2
Pre-culture
Miniprep
Electrophoresis to confirm

10/3
miniprep
PCR
Pre-culture
Purify a gel

10/4
Restriction emzyme fragmation
Purify a gel
Miniprep
Electrophoresis to confirm

10/5
Ligation
Transformation
Purify a gel
Restriction emzyme fragmation

10/6
KIB…miniprep
Electrophoresis to confirm
Purify a gel

10/7
Restriction emzyme fragmation
Ligation
Transformation

10/8
Miniprep
Purify a gel
Restriction emzyme fragmation

10/9
Pre-culture
Purify a gel

0/10
Ligation
Transformation

10/11
pre-culture
Miniprep

10/12
Purify a gel

10/13
Electrophoresis to confirm

10/14
Restriction emzyme fragmation

10/15
Electrophoresis to confirm
Restriction emzyme fragmation

10/16
Electrophoresis to confirm