Difference between revisions of "Team:OUC-China/Communicating"
| Line 171: | Line 171: | ||
<br> | <br> | ||
<h3 class="text-center"><img src="https://static.igem.org/mediawiki/2016/c/cf/T--OUC-China--head-icon1.fw.png" alt="icon">CONSULT Dr. Xu<img src="https://static.igem.org/mediawiki/2016/f/f8/T--OUC-China--head-icon2.fw.png" alt="icon"></h3> | <h3 class="text-center"><img src="https://static.igem.org/mediawiki/2016/c/cf/T--OUC-China--head-icon1.fw.png" alt="icon">CONSULT Dr. Xu<img src="https://static.igem.org/mediawiki/2016/f/f8/T--OUC-China--head-icon2.fw.png" alt="icon"></h3> | ||
| − | <p>At the beginning of our project design, we met a tough problem of choosing chassis. We had two choices, <i>B.subtilis</i> and <i>E.coli</i>. The <i>B.subtilis</i> is a kind of Gram-positive bacterium and is more similar to our prototype---<i> | + | <p>At the beginning of our project design, we met a tough problem of choosing chassis. We had two choices, <i>B.subtilis</i> and <i>E.coli</i>. The <i>B.subtilis</i> is a kind of Gram-positive bacterium and is more similar to our prototype---<i>Clostridium cellulolyticum</i>, but the <i>E. coli</i> is more versatile and widely used. </p> |
| − | <p>We summarized our doubts and consulted Dr. Xu Chenggang, an expert in <i>C.cellulolyticum</i> research. Then we got the advice of comparing more differences that related to our design and he recommended us several useful software. In the process of blasting and comparing enzyme system between | + | <p>We summarized our doubts and consulted Dr. Xu Chenggang, an expert in <i>C. cellulolyticum</i> research. Then we got the advice of comparing more differences that related to our design and he recommended us several useful software. In the process of blasting and comparing enzyme system between them, we found that <i>E. coli</i> has no exoribonuclease that can digest mRNA from 5’ termini, which will be of great benefit to our whole design. With the help of expert and our deep research, we confirmed our chassis and went on with further design.</p> |
<div class="row"> | <div class="row"> | ||
<div class="col-md-2"></div> | <div class="col-md-2"></div> | ||
| Line 184: | Line 184: | ||
<hr> | <hr> | ||
<h3 class="text-center"><img src="https://static.igem.org/mediawiki/2016/c/cf/T--OUC-China--head-icon1.fw.png" alt="icon">JOIN THE CCIC<img src="https://static.igem.org/mediawiki/2016/f/f8/T--OUC-China--head-icon2.fw.png" alt="icon"></h3> | <h3 class="text-center"><img src="https://static.igem.org/mediawiki/2016/c/cf/T--OUC-China--head-icon1.fw.png" alt="icon">JOIN THE CCIC<img src="https://static.igem.org/mediawiki/2016/f/f8/T--OUC-China--head-icon2.fw.png" alt="icon"></h3> | ||
| − | <p>Then in September, we joined the CCiC (Conference of China iGEMer Committee) held by SYSU, which is a communicating platform for Chinese iGEM teams. About 30 teams and 300 iGEMers from all over China gathered together. We shared our design with other iGEMers and | + | <p>Then in September, we joined the CCiC (Conference of China iGEMer Committee) held by SYSU-CHINA, which is a communicating platform for Chinese iGEM teams. About 30 teams and 300 iGEMers from all over China gathered together. We shared our design with other iGEMers and were glad that we received many specific responses, from constructive project design to detailed data process skills. Then according to those suggestions, we modified our original design and added two supplementary proof experiments, which was essential to the integrity of our project.</p> |
<div class="row"> | <div class="row"> | ||
<div class="col-md-2"></div> | <div class="col-md-2"></div> | ||
Revision as of 17:37, 19 October 2016
Communicating & Improving
CONSULT Dr. Xu
At the beginning of our project design, we met a tough problem of choosing chassis. We had two choices, B.subtilis and E.coli. The B.subtilis is a kind of Gram-positive bacterium and is more similar to our prototype---Clostridium cellulolyticum, but the E. coli is more versatile and widely used.
We summarized our doubts and consulted Dr. Xu Chenggang, an expert in C. cellulolyticum research. Then we got the advice of comparing more differences that related to our design and he recommended us several useful software. In the process of blasting and comparing enzyme system between them, we found that E. coli has no exoribonuclease that can digest mRNA from 5’ termini, which will be of great benefit to our whole design. With the help of expert and our deep research, we confirmed our chassis and went on with further design.
Figure 1. 2016 OUC-iGEMers with Dr. Xu
JOIN THE CCIC
Then in September, we joined the CCiC (Conference of China iGEMer Committee) held by SYSU-CHINA, which is a communicating platform for Chinese iGEM teams. About 30 teams and 300 iGEMers from all over China gathered together. We shared our design with other iGEMers and were glad that we received many specific responses, from constructive project design to detailed data process skills. Then according to those suggestions, we modified our original design and added two supplementary proof experiments, which was essential to the integrity of our project.
Figure 2．Open ceremony of CCiC
Cistrons Concerto
Thanks
1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
2.NEW ENGLAND Biolabs
Contact us:
E-mail: oucigem@163.com
Designed and built by @ Jasmine Chen and @ Zexin Jiao
We are OUC-iGEM
