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<br> | <br> | ||
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− | <dd> | + | |
+ | <dt>8/17<br> | ||
+ | <dd>Restriction emzyme fragmation<br> | ||
K592024 B0015 C0051→Eco RI & SpeI<br> | K592024 B0015 C0051→Eco RI & SpeI<br> | ||
I14144 R100 B0015 →XbaI & PstI<br> | I14144 R100 B0015 →XbaI & PstI<br> | ||
EcoRI XbaI PstI →Promega<br> | EcoRI XbaI PstI →Promega<br> | ||
SpeI→Takara<br> | SpeI→Takara<br> | ||
+ | Transcription<br> | ||
<br> | <br> | ||
<dt>8/18<br> | <dt>8/18<br> | ||
− | <dd> | + | <dd>Transcription<br> |
R0051 K592024 I14044 B0015 R0010<br> | R0051 K592024 I14044 B0015 R0010<br> | ||
C0051(psbic3)→E・coli-DH5X<br> | C0051(psbic3)→E・coli-DH5X<br> | ||
− | + | miniprep<br> | |
− | + | ||
− | + | ||
− | + | ||
J04450<br> | J04450<br> | ||
colony PCR<br> | colony PCR<br> | ||
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</dl> | </dl> | ||
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Revision as of 18:35, 19 October 2016
Laborite
K592024 B0015 C0051→Eco RI & SpeI
I14144 R100 B0015 →XbaI & PstI
EcoRI XbaI PstI →Promega
SpeI→Takara
Transcription
R0051 K592024 I14044 B0015 R0010
C0051(psbic3)→E・coli-DH5X
miniprep
J04450
colony PCR
K592024 I14044 B0015 R0010 C0051
Ligation
PCR
R0051 K592024 I14044 B0015 R0010 C0051
J04450
3A assembly
K595024 + I14044
K595024 I14044 C0051 R0010 B0015
purity a gel
preculture
K595024 I14044 C0051 R0010 B0015
K592024 I14044 C0051 R0010 B0015
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
K592024 I14044 C0051 R010 B0015 J04450
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
K592024 I14044 C0051 R010 B0015 J04450
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
K592024 I14044 C0051 R010 B0015 J04450
C0051 R010 B0015X B0015E J04450
Pre-culture
K592024 I14044 J04450
Restriction enzyme treatment
Miniprep
K592024 I14044 J04450
Restriction enzyme treatment
K592024 I14044 J04450
K592024 I14044 J04450
Miniprep
Restriction emzyme fragmation
Miniprep
Purify a gel
Purify a gel
Ligation
Transformation
Restriction emzyme fragmation
Purify a gel
transformation
Pre-culture
Purification of antisensence
Miniprep
Electrophoresis to confirm
PCR
Pre-culture
Purify a gel
Purify a gel
Miniprep
Electrophoresis to confirm
Transformation
Purify a gel
Restriction emzyme fragmation
Electrophoresis to confirm
Purify a gel
Ligation
Transformation
Purify a gel
Restriction emzyme fragmation
Purify a gel
Transformation
Miniprep
Restriction emzyme fragmation