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Revision as of 19:01, 19 October 2016
Labonote
K592024 B0015 C0051→Eco RI & SpeI
I14144 R100 B0015 →XbaI & PstI
EcoRI XbaI PstI →Promega
SpeI→Takara
Transcription
R0051 K592024 I14044 B0015 R0010
C0051(psbic3)→E・coli-DH5X
miniprep
J04450
colony PCR
K592024 I14044 B0015 R0010 C0051
Ligation
PCR
R0051 K592024 I14044 B0015 R0010 C0051
J04450
3A assembly
K595024 + I14044
K595024 I14044 C0051 R0010 B0015
purity a gel
preculture
K595024 I14044 C0051 R0010 B0015
K592024 I14044 C0051 R0010 B0015
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
K592024 I14044 C0051 R010 B0015 J04450
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
K592024 I14044 C0051 R010 B0015 J04450
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
K592024 I14044 C0051 R010 B0015 J04450
C0051 R010 B0015X B0015E J04450
Pre-culture
K592024 I14044 J04450
Restriction enzyme treatment
Miniprep
K592024 I14044 J04450
Restriction enzyme treatment
K592024 I14044 J04450
K592024 I14044 J04450
Miniprep
Restriction emzyme fragmation
Miniprep
Purify a gel
Purify a gel
Ligation
Transformation
Restriction emzyme fragmation
Purify a gel
transformation
Pre-culture
Purification of antisensence
Miniprep
Electrophoresis to confirm
PCR
Pre-culture
Purify a gel
Purify a gel
Miniprep
Electrophoresis to confirm
Transformation
Purify a gel
Restriction emzyme fragmation
Electrophoresis to confirm
Purify a gel
Ligation
Transformation
Purify a gel
Restriction emzyme fragmation
Purify a gel
Transformation
Miniprep
Restriction emzyme fragmation