Difference between revisions of "Team:UChicago/Basic Part"
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| − | We submitted | + | We submitted a plasmid containing both GAL4-KaiC fusion protein and a KaiB protein, linked by a self-cleaving linker:</span></p> |
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<span class="ptitle"><a href="http://parts.igem.org/Part:BBa_K2176002">BBa_K2176002</a></span><br><br> | <span class="ptitle"><a href="http://parts.igem.org/Part:BBa_K2176002">BBa_K2176002</a></span><br><br> | ||
<p><span class="p"> | <p><span class="p"> | ||
| − | This plasmid contains KaiC fused to a Gal4 activation domain, with a self-cleaving peptide linker, P2A, fusing it to KaiB. P2A cleaves itself during translation, causing the KaiC-Gal4AD and the KaiB to separate. We did this to allow us to control the exact ratio of KaiC, KaiB, and KaiA, and make sure it is constant and equal in every cell.</span></p> | + | This plasmid contains KaiC fused to a Gal4 activation domain, with a self-cleaving peptide linker, P2A, fusing it to KaiB. P2A cleaves itself during translation, causing the KaiC-Gal4AD and the KaiB to separate. We did this to allow us to control the exact ratio of KaiC, KaiB, and KaiA, and make sure it is constant and equal in every cell. It is also codon optimized for yeast, and contains an NLS sequence to localize it to the nucleus. This part was synthesized by Genscript.</span></p> |
</div> | </div> | ||
Revision as of 21:15, 19 October 2016
We submitted a plasmid containing both GAL4-KaiC fusion protein and a KaiB protein, linked by a self-cleaving linker:
This plasmid contains KaiC fused to a Gal4 activation domain, with a self-cleaving peptide linker, P2A, fusing it to KaiB. P2A cleaves itself during translation, causing the KaiC-Gal4AD and the KaiB to separate. We did this to allow us to control the exact ratio of KaiC, KaiB, and KaiA, and make sure it is constant and equal in every cell. It is also codon optimized for yeast, and contains an NLS sequence to localize it to the nucleus. This part was synthesized by Genscript.
