Difference between revisions of "Team:Pumas Mexico/Experiments"
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Revision as of 22:00, 19 October 2016
Modified and development by Carolina Cruz, Alejandro Alarcón y Franklin Cruz
Bold Basal Growth Medium
To make A solution:
Add 10g sodium nitrate, 0.03g Magnesium sulphate heptahydrate, 0.1g Sodium chloride, 0.03g Dibasic potassium phosphate, 0.07g Monobasic potassium phosphate and 0.01g Calcium chloride to 400mL distilled water >br>To make trace elementes
Add 8.82g Zinc sulfate heptahydrate, 1.44g Magnesium chloride tetrahydrate and 1.57g Copper sulfate pentahydrate to 1000mL distilled water. Autoclave is recquired to dissolve.
To make B solution
Add 50g Ethylenediaminetetraacetic acid, 31g Potassium Hydroxide and 4.98g Heptahydrate iron sulfate.
Important poinst: If prepare growth medicum add 10mL A solution, 1ml trace elements and 1mL C solution to 936mL distilled water Add 1mL Sulfuric acid to ajust pH
To make a Nutritional stress growth medium add half nitrogen sources.
To make a solid growth medium add 15g Bacteriological to 1000mL growth medium.
TAP growth medium
To make salts:
Add 15.0g Ammonium chloride, 4.0g Magnesium sulphate heptahydrate, and 2.0g Calcium chloride to 1000mL distilled water.
To make phosphate solution.
Add 28.8g Dipotassium phosphate, and 14.4 g Monopotassium phosphate to 1000mL distilled water.
To make trace elements.
Add 8.82 Zinc sulfate heptahydrate, 1.44g Magnesium chloride tetrahydrate, 1.57g Copper sulfate pentahydrate to 1000mL distilled water. Autoclave is recquired to dissolve.
Important points:
If prepare a Growth medium (1000mL), add 2.42g Tris(hydroxymethyl)aminomethane, 25mL TAP, .375mL phosphate solution, 2mL trace elements and 1mL Acetic acid to 1000mL distilled wate.
Add 1mL Sulfuric acid to ajust pH.
To make a Nutritional stress growth medium add half nitrogen sources.
To make a solid growth medium add 15g Bacteriological to 1000mL growth medium.
Describe the experiments, research and protocols you used in your iGEM project.
