Difference between revisions of "Team:Ain shams-Egypt/Attributions"

Line 90: Line 90:
 
  Wet Lab :  Restriction and Ligation, Gibson Assembly, Cell culture Transfection, Gene Sequencing, Bacterial transformation, Plasmid miniprep .  
 
  Wet Lab :  Restriction and Ligation, Gibson Assembly, Cell culture Transfection, Gene Sequencing, Bacterial transformation, Plasmid miniprep .  
 
Shared in the Human Practice role of IGEM Egypt 2016 .<br>
 
Shared in the Human Practice role of IGEM Egypt 2016 .<br>
<b> Maged   :</b> Wet Lab :  Restriction and Ligation, Gibson Assembly, Cell culture Transfection, Gene Sequencing, Bacterial transformation, Plasmid miniprep .  
+
<b>Ahmed Ashry   :</b> Wet Lab :  Restriction and Ligation, Gibson Assembly, Cell culture Transfection, Gene Sequencing, Bacterial transformation, Plasmid miniprep .  
 
Logo design .
 
Logo design .
 
57357  hospital visit poster , flyer and presentation design .
 
57357  hospital visit poster , flyer and presentation design .
Line 103: Line 103:
 
<div class="separator line-separator"><i class="fa fa-leaf"></i></div>
 
<div class="separator line-separator"><i class="fa fa-leaf"></i></div>
 
</div>
 
</div>
<p style="font-size:14pt !important;"><b>Dr. Steven Hussey:</b> Team instructor. Project guidance. Technical assistance.<br>
+
<p style="font-size:14pt !important;"><b>Assistant professor Hend Mohammad Elhelalay :</b> Team founder . Principle instructor . Logistics and facilitating team work .
 
<b>Dr Marwa Matboli Sayed </b> Formulation of study hypothesis.
 
<b>Dr Marwa Matboli Sayed </b> Formulation of study hypothesis.
 
Supervision of study design preparation.
 
Supervision of study design preparation.
Line 111: Line 111:
 
Drafting final results, discussion and conclusion.
 
Drafting final results, discussion and conclusion.
 
Supervision of human practice. <br>
 
Supervision of human practice. <br>
 +
<b>Dr Nashwa Nagy </b> Supervision of Execution of wet lab experiments: Restriction ligation, PCR amplification , Gibson assembly ,Bacterial transformation, Miniprep , Cell culture transfection ,Agarose gel electrophoresis.
 +
Data analysis and interpretation.
 +
Drafting final results, discussion and conclusion.
 +
Molecular biology laboratory techniques workshop  for iGEM students .
 +
<br>
 +
<b>Dr Prof.Dr. Hebatalla Said salem </b> Proposal design revision
 +
Supervision of practical work
 +
Supervision of human practice preparation
 +
Revision of final results and discussion
 +
<br>
  
<b> Professor. Dr. Mahomoud Ahmed Ali </b> Proposal design revision.
+
<b> Professor Dr. Mahomoud Ahmed Ali </b> Proposal design revision.
 
Supervision of practical work.
 
Supervision of practical work.
 
Supervision of human practice preparation.
 
Supervision of human practice preparation.

Revision as of 22:51, 19 October 2016

WATTS-APTAMER - UP_PRETORIA iGEM

WATTS-APTAMER - PRETORIA_UP iGEM

Attributions

The Ain Shams-Egypt team would like to extend special thanks to the numerous people and organizations that supported us throughout this project. We could not have done this without you.


Team members

Mostfa : Team leader ,Wet Lab : Restriction and Ligation, Gibson Assembly, Cell culture Transfection, Gene Sequencing, Bacterial transformation, Plasmid miniprep . Idea development ,System design . Dry Lab : Circuits design, Gibson assembly primers design, Basic and Composite parts design.Work shops ,Shared in Collaboration with zewail university . Shared in the Human Practice role of IGEM Egypt 2016 .
Hend : Wet Lab : Restriction and Ligation, Gibson Assembly, Cell culture Transfection, Gene Sequencing, Bacterial transformation, Plasmid miniprep . Idea development .Work shops . IT team coordinator . Conducting fundraising. Shared in the Human Practice role of IGEM Egypt 2016 .
Mahmoud : Wet Lab : Restriction and Ligation, Gibson Assembly, Cell culture Transfection, Gene Sequencing, Bacterial transformation, Plasmid miniprep . Idea development .Work shops . Video script and scientific data writing. Conducting fundraising . Collaboration with Zewiel University. Team structure development and team hierarchy. Shared in the Human Practice role of IGEM Egypt 2016 .
Nahla : Wet Lab : Restriction and Ligation, Gibson Assembly, Cell culture Transfection, Gene Sequencing, Bacterial transformation, Plasmid miniprep . Idea development .Work shops . Video script and scientific data writing. Team structure development and team hierarchy. Shared in the Human Practice role of IGEM Egypt 2016 .
Sally : Wet Lab : Restriction and Ligation, Gibson Assembly, Cell culture Transfection, Gene Sequencing, Bacterial transformation, Plasmid miniprep . Idea development .Work shops . Video script and scientific data writing. Team structure development and team hierarchy. Leader of humane practice and collaboration . Conducting fundraising.
Khaled: Wet Lab : Restriction and Ligation, Gibson Assembly, Cell culture Transfection, Gene Sequencing, Bacterial transformation, Plasmid miniprep . Idea development .Work shops . Characterization . Plasmid selection. Shared in the Human Practice role of IGEM Egypt 2016 .
Munir : Wet Lab : Restriction and Ligation, Gibson Assembly, Cell culture Transfection, Gene Sequencing, Bacterial transformation, Plasmid miniprep . Video making . Banner design. Creating Facebook page and YouTube channel . Shared in the Human Practice role of IGEM Egypt 2016 .
Ahmed A. Elkader : Wet Lab : Restriction and Ligation, Gibson Assembly, Cell culture Transfection, Gene Sequencing, Bacterial transformation, Plasmid miniprep . Wiki designing and coding . Shared in the Human Practice role of IGEM Egypt 2016 .
Nourhan : Logo and flyer design.Photography. Shared in the Human Practice role of IGEM Egypt 2016 .
Ahmed Hamdy : Preparation of the flow chart of the wet lab synthetic work . Wet Lab : Restriction and Ligation, Gibson Assembly, Cell culture Transfection, Gene Sequencing, Bacterial transformation, Plasmid miniprep . Idea Development . Shared in the Human Practice role of IGEM Egypt 2016 .
Maged : Wet Lab : Restriction and Ligation, Gibson Assembly, Cell culture Transfection, Gene Sequencing, Bacterial transformation, Plasmid miniprep . Idea Development . Shared in the Human Practice role of IGEM Egypt 2016 .
Ahmed El-Sayed : Bioinformatics analysis : to select relevant cirRNA for HCC through cirBase and Circ2Traits databases. and to retrieve competing endogenous RNA specific for HCC through Circular RNA interactome database and Starbase database. Wet Lab : Restriction and Ligation, Gibson Assembly, Cell culture Transfection, Gene Sequencing, Bacterial transformation, Plasmid miniprep . Shared in the Human Practice role of IGEM Egypt 2016 .
Ahmed Ashry : Wet Lab : Restriction and Ligation, Gibson Assembly, Cell culture Transfection, Gene Sequencing, Bacterial transformation, Plasmid miniprep . Logo design . 57357 hospital visit poster , flyer and presentation design . T-shirt Design and printing . T-shirt Design and printing. Photography. Shared in the Human Practice role of IGEM Egypt 2016 . Visited Portsaid international School (PIS)


Instructors

Assistant professor Hend Mohammad Elhelalay : Team founder . Principle instructor . Logistics and facilitating team work . Dr Marwa Matboli Sayed Formulation of study hypothesis. Supervision of study design preparation. Supervision of bioinformatics analysis to retrieve competing endogenous RNA network specific to hepatocellular carcinoma. Supervision of wet lab . Data analysis and interpretation. Drafting final results, discussion and conclusion. Supervision of human practice.
Dr Nashwa Nagy Supervision of Execution of wet lab experiments: Restriction ligation, PCR amplification , Gibson assembly ,Bacterial transformation, Miniprep , Cell culture transfection ,Agarose gel electrophoresis. Data analysis and interpretation. Drafting final results, discussion and conclusion. Molecular biology laboratory techniques workshop for iGEM students .
Dr Prof.Dr. Hebatalla Said salem Proposal design revision Supervision of practical work Supervision of human practice preparation Revision of final results and discussion
Professor Dr. Mahomoud Ahmed Ali Proposal design revision. Supervision of practical work. Supervision of human practice preparation. Data analysis and interpretation. Revision of final results and discussion.
Professor Dr. Hany Lotfy Hafez Supervision of students during practical work Facilitation of logistics issues for the students. Final approval of the results and discussion.



Thylakoid protein selection

Dr Michal Gwizdala and Dr Tjaart Kruger for advice given on choosing the appropriate protein in the thylakoid membrane to target for our aptamer design.



Lab support

Mr. Jonathan Botha: valuable input and advice on experiments and which protocols to use for transformation. General help on lab safety and good lab practice.
Mr. Donovin Coles: Advice on restriction enzyme digest procedures.
Mr. Lazarus Takawira: General advice on lab procedures.
Ms. Elodie Ekoka: Valuable input and advice on thylakoid extraction procedures.
Ms. Katrien Brown: General advice on lab procedures.
Mr. Alan Hall and Dr. Eudri Venter: Technical expertise with confocal microscopy experiments for visualising thylakoid binding to the aptamers.
Prof. N. Manyala, Dr. J. Dangbegnon, Dr. A Bello, Mr J. Madito and Mr A.A Khaleed from the Carbon Based Nano Material Research Group for their advice and help with regards to graphene.
Dr. T. Stander from the Carl and Emily Fuchs Institute for Microelectronics (CEFIM) for allowing us to use their current measuring equipment to test our cells’ functioning.
Ms. Elna Cowley, Lab Manager: Helped with Lab safety training.



Modelling

Dr Tjaart Krüger for advice given with modelling.



Human Practices support

Dr Karen Surridge-Talbot, Centre Manager: Renewable Energy Centre of Research & Development Clean Energy at the South African National Energy Development Institute (SANEDI).
Mrs Marie Venter, Natural and Agricultural Sciences Marketing and Communication, University of Pretoria.
Mrs Ntsatsi Mantsho, Department of Research and Innovation Support, University of Pretoria.
Mrs Dikeledi, Lesedi Power Project
Tuks FM
UP with Science, University of Pretoria
Mr Johann Louw, Kumba Virtual Reality Centre, Department of Mining Engineering, University of Pretoria
Dr Bertie Meyer, Aix Marseille documentary
Mr Roland Henwood, Aix Marseille documentary




Wiki support

Team William & Mary 2015 - CSS and inspiration from their beautiful wiki.
Eric Herot - "Okra Seedling Time Lapse" Video. Creative Commons Attribution license (reuse allowed).
Wikimedia Commons - MacQuarie University, Aix-Marseille University, Heidelberg University images on collaborations page. FeesMustFall image on Human Practices Silver page. Creative Commons Attribution-Share Alike license.
Team BostonU 2014 - Medal images and inspiration.



Funding Support

Sappi
The University of Pretoria
The Department of Science & Technology
Inqaba Biotec
Thermo Fisher Scientific
Whitehead Scientific
Integrated DNA Technologies
The Forestry and Agricultural Biotechnology Institute
The Forest Molecular Genetics Programme
The African Centre for Gene Technologies
The South African National Energy Development Institute



Donations

Mr Plastic Group Bedfordview: Clifford Van Niekerk, for donating perspex for the construction of our PBEC.
Dr SG Radhakrishnan, Department of Chemistry, Faculty of Natural and Agricultural sciences for kindly donating the proton exchange membrane (Nafion 117) we needed to test our cell.
Dr Karen Surridge-Talbot and SANEDI for the generous donation of a stall at the Women in Energy Business Summit.
Prof. N. Manyala, Dr. J. Dangbegnon, Dr. A Bello, Mr J. Madito and Mr A.A Khaleed from the Carbon Based Nano Material Research Group for assisting the team by growing graphene foam on request at no expense to the team.




WATTS-APTAMER - UP_PRETORIA iGEM