Difference between revisions of "Team:Duesseldorf/Experiments"
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Revision as of 01:05, 20 October 2016
Lab Book
These are the protocols we used. If changes have been made, they are documented in the respective lab diary.
- AQUA Cloning
- Colony PCR
- Qiagen Miniprep
- Promega Miniprep
- PCR gel cleanup Qiagen
- PCR gel cleanup Promega
- PCR purification
- Phusion PCR
- pJET blund end cloning
- pJET sticky end cloning
- Plate
- Restriction digest
- Site- Directed Mutagenesis
- Test restriction digest
- Transformation
- Mammal Cell Splitting
- Mammal Cell Transfection
- Boiling Prep
- Topo Cloning
Our lab diary
- Noch ausfüllen!
