Difference between revisions of "Team:OUC-China/Part Collection"

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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<span class="caret"></span></a>
 
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<span class="caret"></span></a>
 
<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
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<li><a href="https://2016.igem.org/Team:OUC-China/Project">Introduction</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Description">Description</a></li>
 
<li><a href="https://2016.igem.org/Team:OUC-China/Design">Design</a></li>
 
<li><a href="https://2016.igem.org/Team:OUC-China/Design">Design</a></li>
 
<li><a href="https://2016.igem.org/Team:OUC-China/Proof">Proof of concept </a></li>
 
<li><a href="https://2016.igem.org/Team:OUC-China/Proof">Proof of concept </a></li>
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<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
 
<li><a href="https://2016.igem.org/Team:OUC-China/Human_Practices">Overview</a></li>
 
<li><a href="https://2016.igem.org/Team:OUC-China/Human_Practices">Overview</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Communicating">Communicating & Improving</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Communicating_&_improving">Communicating & improving</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Investigating">Investigating & Promoting</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Investigating_&_promoting">Investigating & promoting</a></li>
 
<li><a href="https://2016.igem.org/Team:OUC-China/Potential_application">Potential application</a></li>
 
<li><a href="https://2016.igem.org/Team:OUC-China/Potential_application">Potential application</a></li>
 
</ul>
 
</ul>
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</nav>
 
</nav>
 
<div class="clearfix"></div>
 
<div class="clearfix"></div>
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<div class="jumbotron">
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        <div class="img_banner"><img src="https://static.igem.org/mediawiki/2016/0/0d/T--OUC-China--Part_Banner.jpg" class="img-responsive" alt="parts-banner"></div>
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<h1>Part collection</h1>
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<h3 class="text-center">Part Collection</h3>
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<p style="width:60%;margin:0 auto;"><h3>Introduction</h3>
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<p style="font-size:20px;font-family:'Calibri'">We devoted to constructing a library of stem-loops to regulate the expression of protein and exploring a toolkit.<br>We tested both native stem-loops and designed stem-loops and we found some parts can coordinate the expression well. Therefore, we submitted these parts to iGEM parts registry with detailed characterization. Then, with the demands of bigger library and more detailed data, we collected these measured stem-loops with various folding free energy to work on constructing a toolkit. </p>
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 +
<div class="table-responsive" style="width:60%;margin:0 auto;" >
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<table class="table table-bordered">
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  <tr>
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<td>Part Number</td>
 +
<td>Type</td>
 +
<td>Description</td>
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<td>Designer</td>
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<td>Length(bp)</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047001">BBa_K2047001</a></td>
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<td>Composite</td>
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<td>GFP_Stem-loop with free energy of -25.6 measured by Mfold</td>
 +
<td>Yu Jiang</td>
 +
<td>801</td>
 +
  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047003">BBa_K2047003</a></td>
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<td>Composite</td>
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<td>GFP_Stem-loop with free energy of -38.7measured by Mfold</td>
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<td>Yu Jiang</td>
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<td>809</td>
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  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047101">BBa_K2047101</a></td>
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<td>Composite</td>
 +
<td>GFP_Stem-loop with free energy of -30.1 measured by Mfold</td>
 +
<td>Yu Jiang</td>
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<td>814</td>
 +
  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047102">BBa_K2047102</a></td>
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<td>Composite</td>
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<td>GFP_Stem-loop with free energy of -14.9 measured by Mfold</td>
 +
<td>Yu Jiang</td>
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<td>800</td>
 +
  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047103">BBa_K2047103</a></td>
 +
<td>Composite</td>
 +
<td>GFP_Stem-loop with free energy of -38.8 measured by Mfold</td>
 +
<td>Yu Jiang</td>
 +
<td>821</td>
 +
  </tr>
 +
  <tr>
 +
<td><a href="http://parts.igem.org/Part:BBa_K2047104">BBa_K2047104</a></td>
 +
<td>Composite</td>
 +
<td>GFP_Stem-loop with free energy of -34.4 measured by Mfold</td>
 +
<td>Yu Jiang</td>
 +
<td>818</td>
 +
  </tr>
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  <tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2047106">BBa_K2047106</a></td>
 +
<td>Composite</td>
 +
<td>GFP_Stem-loop with free energy of -38.5 measured by Mfold</td>
 +
<td>Yu Jiang</td>
 +
<td>822</td>
 +
  </tr>
 +
  <tr>
 +
<td><a href="http://parts.igem.org/Part:BBa_K2047107">BBa_K2047107</a></td>
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<td>Composite</td>
 +
<td>GFP_Stem-loop with free energy of -44.9 measured by Mfold</td>
 +
<td>Yu Jiang</td>
 +
<td>798</td>
 +
  </tr>
 +
  <tr>
 +
<td><a href="http://parts.igem.org/Part:BBa_K2047108">BBa_K2047108</a></td>
 +
<td>Composite</td>
 +
<td>GFP_Stem-loop with free energy of -38.8 measured by Mfold</td>
 +
<td>Yu Jiang</td>
 +
<td>824</td>
 +
  </tr>
 +
</table>
 
</div>
 
</div>
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<div class="row">
 
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<div class="col-md-6 text-center">
 
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<a href="http://parts.igem.org/Part:BBa_K2047001">BBa_K2047001</a><br />
 
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<a href="http://parts.igem.org/Part:BBa_K2047002">BBa_K2047002</a><br />
 
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<a href="http://parts.igem.org/Part:BBa_K2047003">BBa_K2047003</a><br />
 
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<a href="http://parts.igem.org/Part:BBa_K2047004">BBa_K2047004</a><br />
 
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<a href="http://parts.igem.org/Part:BBa_K2047005">BBa_K2047005</a><br />
 
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<a href="http://parts.igem.org/Part:BBa_K2047006">BBa_K2047006</a><br />
 
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<a href="http://parts.igem.org/Part:BBa_K2047007">BBa_K2047007</a><br />
 
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<a href="http://parts.igem.org/Part:BBa_K2047008">BBa_K2047008</a><br />
 
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</div>
 
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<div class="col-md-6 text-center">
 
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<a href="http://parts.igem.org/Part:BBa_K2047101">BBa_K2047101</a><br />
 
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<a href="http://parts.igem.org/Part:BBa_K2047102">BBa_K2047102</a><br />
 
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<a href="http://parts.igem.org/Part:BBa_K2047103">BBa_K2047103</a><br />
 
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<a href="http://parts.igem.org/Part:BBa_K2047104">BBa_K2047104</a><br />
 
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<a href="http://parts.igem.org/Part:BBa_K2047105">BBa_K2047105</a><br />
 
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<a href="http://parts.igem.org/Part:BBa_K2047106">BBa_K2047106</a><br />
 
−
<a href="http://parts.igem.org/Part:BBa_K2047107">BBa_K2047107</a><br />
 
−
<a href="http://parts.igem.org/Part:BBa_K2047108">BBa_K2047108</a><br />
 
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</div>
 
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</div>
 
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<br />
 
 
<br />
 
<br />
 
<br />
 
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<div class="col-md-1"></div>
 
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<div class="col-md-5">
 
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<h3>Thanks:</h3>
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<h3>Thanks</h3>
 
<p>1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p>
 
<p>1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p>
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<p>2.NEW ENGLAND Biolabs</p>
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<p>2.NEW ENGLAND Biolabs</p>
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<p>3.Genscript</p>
 
</div>
 
</div>
 
<div class="col-md-5 Contact">
 
<div class="col-md-5 Contact">
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</div>
 
</div>
 
<div class="oucBottom">
 
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Revision as of 02:28, 20 October 2016

Part collection

parts-banner

Part Collection

Introduction

We devoted to constructing a library of stem-loops to regulate the expression of protein and exploring a toolkit.
We tested both native stem-loops and designed stem-loops and we found some parts can coordinate the expression well. Therefore, we submitted these parts to iGEM parts registry with detailed characterization. Then, with the demands of bigger library and more detailed data, we collected these measured stem-loops with various folding free energy to work on constructing a toolkit.

Part Number Type Description Designer Length(bp)
BBa_K2047001 Composite GFP_Stem-loop with free energy of -25.6 measured by Mfold Yu Jiang 801
BBa_K2047003 Composite GFP_Stem-loop with free energy of -38.7measured by Mfold Yu Jiang 809
BBa_K2047101 Composite GFP_Stem-loop with free energy of -30.1 measured by Mfold Yu Jiang 814
BBa_K2047102 Composite GFP_Stem-loop with free energy of -14.9 measured by Mfold Yu Jiang 800
BBa_K2047103 Composite GFP_Stem-loop with free energy of -38.8 measured by Mfold Yu Jiang 821
BBa_K2047104 Composite GFP_Stem-loop with free energy of -34.4 measured by Mfold Yu Jiang 818
BBa_K2047106 Composite GFP_Stem-loop with free energy of -38.5 measured by Mfold Yu Jiang 822
BBa_K2047107 Composite GFP_Stem-loop with free energy of -44.9 measured by Mfold Yu Jiang 798
BBa_K2047108 Composite GFP_Stem-loop with free energy of -38.8 measured by Mfold Yu Jiang 824


Cistrons Concerto

Thanks

1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences

2.NEW ENGLAND Biolabs

3.Genscript

Contact us:

E-mail: oucigem@163.com

Designed and built by @ Jasmine Chen and @ Zexin Jiao

We are OUC-iGEM logo-one logo-two