Difference between revisions of "Team:William and Mary/Results"

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Results
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  Over the summer we successfully characterized each individual component of the Circuit Control Toolbox. Each of these components can be used either individually or in concert to orthogonally modify the output of transfer functions of an arbitrary genetic circuit. We also characterized many existing parts and created a predictive mathematical model of molecular titration. The entirety of the circuit toolbox as well as the parts used to characterized them has been submitted to the registry, on a unified backbone designed for ease of use with Gibson Assembly.
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<b>Achievements</b>
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<b>*</b> Characterized four orthogonal methods to control transfer functions.
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<b>1.</b> The ribozyme RiboJ was used to insulate circuits and characterization from genetic context.
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<b>2.</b> A synthetic enhancer system was characterized, allowing for multistate response.
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<b>3.</b> The Community Library of RBSs was characterized under a variety of conditions, using RiboJ to ensure that results were generalizable. These RBSs can be used to tune the amplitude of the transfer function of an arbitrary genetic circuit.
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<b>4.</b> Molecular titration was characterized using a repeat sequence array, which allows an arbitrary genetic circuit to shift it’s transfer function along the x axis.
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<b>*</b> Created a suite of parts that allows for the construction of variable length repeat LacO or TetO arrays.
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<b>*</b> Participated in the Interlab Measurement Project.
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<b>*</b> Created a mathematical model of molecular titration of arbitrary length repeat arrays.
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<p>Here you can describe the results of your project and your future plans. </p>
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<h5>What should this page contain?</h5>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project </li>
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<li> Considerations for replicating the experiments </li>
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<h5> Project Achievements </h5>
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<ul>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<h5>Inspiration</h5>
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<p>See how other teams presented their results.</p>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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Revision as of 02:48, 20 October 2016


...

Results

Over the summer we successfully characterized each individual component of the Circuit Control Toolbox. Each of these components can be used either individually or in concert to orthogonally modify the output of transfer functions of an arbitrary genetic circuit. We also characterized many existing parts and created a predictive mathematical model of molecular titration. The entirety of the circuit toolbox as well as the parts used to characterized them has been submitted to the registry, on a unified backbone designed for ease of use with Gibson Assembly.

Achievements

* Characterized four orthogonal methods to control transfer functions.

1. The ribozyme RiboJ was used to insulate circuits and characterization from genetic context.

2. A synthetic enhancer system was characterized, allowing for multistate response.

3. The Community Library of RBSs was characterized under a variety of conditions, using RiboJ to ensure that results were generalizable. These RBSs can be used to tune the amplitude of the transfer function of an arbitrary genetic circuit.

4. Molecular titration was characterized using a repeat sequence array, which allows an arbitrary genetic circuit to shift it’s transfer function along the x axis.

* Created a suite of parts that allows for the construction of variable length repeat LacO or TetO arrays.

* Participated in the Interlab Measurement Project.

* Created a mathematical model of molecular titration of arbitrary length repeat arrays.