Difference between revisions of "Team:William and Mary/Interlab"
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<img src="https://static.igem.org/mediawiki/parts/c/c3/T--William_and_Mary--Forum.jpg" width="600px"> | <img src="https://static.igem.org/mediawiki/parts/c/c3/T--William_and_Mary--Forum.jpg" width="600px"> | ||
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In addition to participating in the Interlab Study, we also wanted to determine if there would be a noticeable difference | In addition to participating in the Interlab Study, we also wanted to determine if there would be a noticeable difference | ||
between measurements taken at Midlog and measurements taken on those same samples at Saturation. | between measurements taken at Midlog and measurements taken on those same samples at Saturation. | ||
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<img src="https://static.igem.org/mediawiki/parts/c/c3/T--William_and_Mary--Forum.jpg" width="600px"> | <img src="https://static.igem.org/mediawiki/parts/c/c3/T--William_and_Mary--Forum.jpg" width="600px"> | ||
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We noticed that although there were differences between the midlog and saturation measurements, they were not very drastic | We noticed that although there were differences between the midlog and saturation measurements, they were not very drastic | ||
across the different devices when looking at bulk population-level mean measurements, with the exception of the negative control. | across the different devices when looking at bulk population-level mean measurements, with the exception of the negative control. | ||
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<img src="https://static.igem.org/mediawiki/parts/5/5e/T--William_and_Mary--LSBDemo.png" width="500px"> | <img src="https://static.igem.org/mediawiki/parts/5/5e/T--William_and_Mary--LSBDemo.png" width="500px"> | ||
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| + | <p class='large' style="padding-left:40px; text-align: center; padding-top: 20px; color: #A9A9A9; font-size: .75em !important;"> | ||
| + | Fig. 3: Representative single-cell histograms of absolute fluorescence levels for the same sample at midlog phase vs. at saturation phase | ||
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Revision as of 02:58, 20 October 2016
We participated in the Interlab Measurement Study using Flow Cytometry analysis on a FACS (Fluorescence-Activated Cell Sorter)
machine following the protocol provided by iGEM. Our results are presented here:
In addition to participating in the Interlab Study, we also wanted to determine if there would be a noticeable difference
between measurements taken at Midlog and measurements taken on those same samples at Saturation.
We noticed that although there were differences between the midlog and saturation measurements, they were not very drastic
across the different devices when looking at bulk population-level mean measurements, with the exception of the negative control.
However, because we measured the devices with flow cytometry, we were additionally able to assess the changes in population
heterogeneity which occur with between midlog phase and saturation phase. We found that the midlog populations tended to be more
unimodal than the saturation populations.
Fig. 3: Representative single-cell histograms of absolute fluorescence levels for the same sample at midlog phase vs. at saturation phase
Interlab
