Difference between revisions of "Team:UFAM-UEA Brazil/Project/NewPromoters"
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<p id="t2">Different regions for interaction of the promoter with RNA polymerase were described as represented in the figure 1 bellow, such as: up element, sequences of -35 and -10 hexamer, extended -10 element, discriminator. </p> | <p id="t2">Different regions for interaction of the promoter with RNA polymerase were described as represented in the figure 1 bellow, such as: up element, sequences of -35 and -10 hexamer, extended -10 element, discriminator. </p> | ||
<center><img src="https://static.igem.org/mediawiki/2016/thumb/8/84/UFAM_UEA_PROJECT_PROMOTER1.png/800px-UFAM_UEA_PROJECT_PROMOTER1.png"></center> | <center><img src="https://static.igem.org/mediawiki/2016/thumb/8/84/UFAM_UEA_PROJECT_PROMOTER1.png/800px-UFAM_UEA_PROJECT_PROMOTER1.png"></center> | ||
| − | < Figure 1 – Specific sequences for interaction between promoters sequences and RNA polymerase> | + | <p> Figure 1 – Specific sequences for interaction between promoters sequences and RNA polymerase </p> |
<p id="t2">Together, these regions specify the RNA polymerase recognition and interaction to the promoter. Furthermore, two others elements control the transcription of certain gene or set of genes: the repressor proteins and its operators. Many steps of this process can be also highly regulated by accessory protein factors and small ligands, temperature, salt and solute concentrations, and other environmental variables. </p> | <p id="t2">Together, these regions specify the RNA polymerase recognition and interaction to the promoter. Furthermore, two others elements control the transcription of certain gene or set of genes: the repressor proteins and its operators. Many steps of this process can be also highly regulated by accessory protein factors and small ligands, temperature, salt and solute concentrations, and other environmental variables. </p> | ||
| + | <p id="t2">About Operon Mer, it’s regulated by MerR repressor protein that, in the absence of mercury, binds on a promoter-operator-MerR complex, in which the RNA polymerase is prevented from recognizing the promoter sequence, not having genetical transcription. However, in the presence of mercury, it dislocates from the promoter region and binds to it, being allosterically altered, as represented below. </p> | ||
| + | |||
| + | <p id="t2">This unique system of genetic regulated expression sensible to the mercury absence and presence, inactivating and activating, inspired us to design new promoter sequences, merging the well documented and widely used promoter sequences with mer operator. We designed 8 new promoters, the first and only collection of MerR regulated promoters, sensible to mercury, of iGEM!</p> | ||
| + | <p id="t2">The new designed promoters were merged with Tac promoter and JK26 promoter, promoters that interact with RNA polymerase on exponential and stationary phase of bacterial growth. Check out the synthetic genetic circuits on the videos below.</p> | ||
</br> | </br> | ||
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</br> | </br> | ||
</div> | </div> | ||
| + | <p id="t2">We characterized its efficiency through the RFP regulated expression! Check this out in results!</p> | ||
</br> | </br> | ||
Revision as of 03:53, 20 October 2016
New Promoters Library
Description
Transcription is the first step of genetic expression and thus is one of greater and important processes of life. Promoters are DNA sequences that controls the transcription initiation and mRNA production of all genes, recognized and synthetized, respectively, by the enzyme RNA polymerase.
Different regions for interaction of the promoter with RNA polymerase were described as represented in the figure 1 bellow, such as: up element, sequences of -35 and -10 hexamer, extended -10 element, discriminator.
Figure 1 – Specific sequences for interaction between promoters sequences and RNA polymerase
Together, these regions specify the RNA polymerase recognition and interaction to the promoter. Furthermore, two others elements control the transcription of certain gene or set of genes: the repressor proteins and its operators. Many steps of this process can be also highly regulated by accessory protein factors and small ligands, temperature, salt and solute concentrations, and other environmental variables.
About Operon Mer, it’s regulated by MerR repressor protein that, in the absence of mercury, binds on a promoter-operator-MerR complex, in which the RNA polymerase is prevented from recognizing the promoter sequence, not having genetical transcription. However, in the presence of mercury, it dislocates from the promoter region and binds to it, being allosterically altered, as represented below.
This unique system of genetic regulated expression sensible to the mercury absence and presence, inactivating and activating, inspired us to design new promoter sequences, merging the well documented and widely used promoter sequences with mer operator. We designed 8 new promoters, the first and only collection of MerR regulated promoters, sensible to mercury, of iGEM!
The new designed promoters were merged with Tac promoter and JK26 promoter, promoters that interact with RNA polymerase on exponential and stationary phase of bacterial growth. Check out the synthetic genetic circuits on the videos below.
We characterized its efficiency through the RFP regulated expression! Check this out in results!
Results
