Difference between revisions of "Team:FAFU-CHINA/Basic Part"

 
Line 563: Line 563:
  
  
−
  <script>
+
 
−
if (!window.ga) {
+
−
  (function(i,s,o,g,r,a,m){i['GoogleAnalyticsObject']=r;i[r]=i[r]||function(){
+
−
  (i[r].q=i[r].q||[]).push(arguments)},i[r].l=1*new Date();a=s.createElement(o),
+
−
  m=s.getElementsByTagName(o)[0];a.async=1;a.src=g;m.parentNode.insertBefore(a,m)
+
−
  })(window,document,'script','//www.google-analytics.com/analytics.js','ga');
+
−
 
+
−
  window.ga('create', 'UA-42690126-5', 'auto');
+
−
  window.ga('require', 'linkid', 'linkid.js');
+
−
  window.ga('require', 'displayfeatures');
+
−
  window.ga('send', 'pageview');
+
−
} else {
+
−
  window.ga('send', 'pageview', window.location.pathname+window.location.search);
+
−
}
+
−
 
+
−
  </script>
+
 
   <script>
 
   <script>
 
   $('#page-contents a').click(function() {
 
   $('#page-contents a').click(function() {

Latest revision as of 06:36, 8 November 2016

Team FAFU-CHINA

 Basic Part

Cry4Aa4+Extended FMDV 2A-pChlamy_3

Gene Name

Cry4Aa4+Extended FMDV 2A

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

3673bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs1.png

 

T--FAFU-CHINA--bs2.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

Cry10Aa4+Extended FMDV 2A-pChlamy_3

Gene Name

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

2158bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs3.png

 

T--FAFU-CHINA--bs4.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

Cry11Aa4+Extended FMDV 2A-pChlamy_3

Gene Name

Cry11Aa4+ Extended FMDV 2A

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

2071bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs3.png

 

T--FAFU-CHINA--bs6.png

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

Cyt1-pChlamy_3

Gene Name

Cyt1

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

762bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs3.png

 

T--FAFU-CHINA--bs8.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

 

Cyt2-pChlamy_3

Gene Name

Cyt1

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

801bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

T--FAFU-CHINA--bs3.png

 

T--FAFU-CHINA--bs10.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

 

 

 

EGFP-pChlamy_3

Gene Name

06-EGFP（TAA）

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

732bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs3.png

T--FAFU-CHINA--bs12.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA