Difference between revisions of "Team:Paris Saclay/Notebook/July/25"
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Revision as of 09:25, 25 July 2016
Contents
Wednesday 20th July
Lab work
Visualization
Low Fidelity Dreamtaq PCR of transformed DH5α with pPS16_002
By Mathilde
A DreamTaq PCR was made with the transformed culture pPS16_002 re-plated the day before. The usual protocol was followeed with Tm at 57°c and 5min for the initial denaturation.
We divided up the PCR mix in 6 PCR tubes and added in each one a different clone from the transformed culture. The picked colonies were re-plated on a petri dish medium LB + Ampicillin (50µg/mL) + xGal/IPTG (1/1000).
Results : PCR products expected were :
| Plasmid | pPS16_002 |
|---|---|
| Bande Size pb | 960 |
