User contributions
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- 10:14, 25 August 2016 (diff | hist) . . (+1) . . Team:Paris Saclay/Notebook/August/22 (→Device 1 culture)
- 10:13, 25 August 2016 (diff | hist) . . (+1) . . Team:Paris Saclay/Notebook/August/22 (→Transformation into DH5a cells)
- 10:13, 25 August 2016 (diff | hist) . . (+21) . . Team:Paris Saclay/Notebook/August/22 (→pPS16_016 ligation product transformation in DH5a)
- 10:11, 25 August 2016 (diff | hist) . . (+14) . . Team:Paris Saclay/Notebook/August/22 (→Purification of PSB1C3 with kit)
- 10:10, 25 August 2016 (diff | hist) . . (+6) . . Team:Paris Saclay/Notebook/August/22 (→Dream Taq PCR of GFP1-9 cloned in the pSB1C3)
- 10:09, 25 August 2016 (diff | hist) . . (+16) . . Team:Paris Saclay/Notebook/August/22 (→Purification on gel of gBlocks 3 and 4)
- 10:08, 25 August 2016 (diff | hist) . . (+23) . . Team:Paris Saclay/Notebook/August/22 (→Sequencing of 1.2, FKBP, FRB)
- 10:07, 25 August 2016 (diff | hist) . . (+18) . . Team:Paris Saclay/Notebook/August/22 (→Dream Taq PCR of pPS16_016)
- 10:07, 25 August 2016 (diff | hist) . . (+56) . . Team:Paris Saclay/Notebook/August/22 (→Low fidelity Dreamn Taq PCR of pPS16_016)
- 11:36, 22 August 2016 (diff | hist) . . (+4) . . Team:Paris Saclay/Notebook/August/19 (→Colony and Extraction product DreamTaq PCR)
- 11:30, 22 August 2016 (diff | hist) . . (+1) . . Team:Paris Saclay/Notebook/August/19 (→Plasmids extraction)
- 11:28, 22 August 2016 (diff | hist) . . (-4) . . Team:Paris Saclay/Notebook/August/19 (→Q5 PCR on fragments 3 and 4)
- 14:06, 19 August 2016 (diff | hist) . . (0) . . Team:Paris Saclay/Notebook/August/11 (→Transformation of DH5a cells with pPS16_009)
- 14:05, 19 August 2016 (diff | hist) . . (+54) . . Team:Paris Saclay/Notebook/August/11 (→Transformation of DH5a cells with pPS16_009)
- 10:30, 19 August 2016 (diff | hist) . . (-1) . . Team:Paris Saclay/Notebook/August/17 (→Digestion of pPS16_013 clones 2 and 3)
- 08:53, 19 August 2016 (diff | hist) . . (+1) . . Team:Paris Saclay/Notebook/August/18 (→B-Galactosidase and luciferase test on transformed BL21)
- 08:52, 19 August 2016 (diff | hist) . . (+105) . . Team:Paris Saclay/Notebook/August/18 (→pPS16_002, pPS16_010, pPS16_013 and pPS16_014 PCR with pJET)
- 08:39, 19 August 2016 (diff | hist) . . (+11) . . Team:Paris Saclay/Notebook/August/18 (→Samples preparation for sequencing)
- 08:37, 19 August 2016 (diff | hist) . . (+61) . . Team:Paris Saclay/Notebook/August/18 (→Q5 PCR on fragment 3-4 and Q5 joining PCR on with fragment 3 and fragment 4)
- 08:31, 19 August 2016 (diff | hist) . . (+2) . . Team:Paris Saclay/Notebook/August/18 (→Digested fragment 3 ligation with fragment 4)
- 08:29, 19 August 2016 (diff | hist) . . (+3) . . Team:Paris Saclay/Notebook/August/18 (→Samples preparation for sequencing)
- 12:56, 18 August 2016 (diff | hist) . . (+2) . . Team:Paris Saclay/Notebook/August/5 (→PCR Clean-up with the NucleoSpin kit)
- 12:55, 18 August 2016 (diff | hist) . . (+1) . . Team:Paris Saclay/Notebook/August/5 (→Q5 PCR on pPS16_003, pPS16_004, pPS16_006 and pPS16_007)
- 12:54, 18 August 2016 (diff | hist) . . (+41) . . Team:Paris Saclay/Notebook/August/5 (→DNA Extraction of DS-NMcasN- and pPS_001)
- 12:54, 18 August 2016 (diff | hist) . . (+41) . . Team:Paris Saclay/Notebook/August/5 (→DNA Extraction of DS-TDcasN- and DS-SPcasN-)
- 12:44, 18 August 2016 (diff | hist) . . (+50) . . Team:Paris Saclay/Notebook/August/4 (→DNA Extraction of pPS16_001, pPS16_002, pPS16_003, pPS16_009,DS-NMcasN-, DS-TDcasN-, DS-SPcasN-)
- 11:07, 18 August 2016 (diff | hist) . . (+10) . . Team:Paris Saclay/Notebook/August/4 (→PCR Clean-up with the NucleoSpin kit)
- 11:04, 18 August 2016 (diff | hist) . . (+4) . . Team:Paris Saclay/Notebook/August/3 (→High fidelity Q5 PCR on pPS16_004, pPS16_006 and pPS16_007)
- 11:01, 18 August 2016 (diff | hist) . . (+3) . . Team:Paris Saclay/Notebook/August/2 (→Sequencing of the gBlock 4.2)
- 10:57, 18 August 2016 (diff | hist) . . (+17) . . Team:Paris Saclay/Notebook/August/1 (→New gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB insertion in puc19)
- 10:56, 18 August 2016 (diff | hist) . . (+131) . . Team:Paris Saclay/Notebook/August/12 (→Dreamtaq PCR on puc19, detection, FRB, FKBP, SgRNAst, SgRNAnm and gBlock 1.2)
- 10:40, 18 August 2016 (diff | hist) . . (-1) . . Team:Paris Saclay/Notebook/August/11 (→DreamTaq PCR of transformed cell with pPS16_002,008,010, 011, 012, 013, 014 and puC 19)
- 10:38, 18 August 2016 (diff | hist) . . (+81) . . Team:Paris Saclay/Notebook/August/11 (→High Fidelity Phusion PCR of transformed cell with ligation 2 and 3)
- 10:35, 18 August 2016 (diff | hist) . . (-27) . . Team:Paris Saclay/Notebook/August/11 (→pPS16_010 extraction)
- 10:34, 18 August 2016 (diff | hist) . . (-10) . . Team:Paris Saclay/Notebook/August/11 (→pPS16_010 extraction)
- 10:23, 18 August 2016 (diff | hist) . . (+2) . . Team:Paris Saclay/Notebook/August/10 (→Phusion PCR on the ligation products)
- 10:22, 18 August 2016 (diff | hist) . . (-1) . . Team:Paris Saclay/Notebook/August/10 (→Phusion PCR on the ligation products)
- 10:19, 18 August 2016 (diff | hist) . . (+4) . . Team:Paris Saclay/Notebook/August/9 (→pUC19 ligation with gBlocks ATG linker RFB, detection, St sgRNA, ATG linker FKBP, Nm sgRNA, 1.2 and 4.2)
- 10:18, 18 August 2016 (diff | hist) . . (+1) . . Team:Paris Saclay/Notebook/August/9 (→Phusion PCR on pPS16_006 and pPS16_007)
- 09:58, 18 August 2016 (diff | hist) . . (+133) . . Team:Paris Saclay/Notebook/August/8 (→Migration of DNA plasmid extracted)
- 09:52, 18 August 2016 (diff | hist) . . (+5) . . Team:Paris Saclay/Notebook/August/8 (→Liquid cultures of bacteria containing plasmid to extract)
- 09:51, 18 August 2016 (diff | hist) . . (+18) . . Team:Paris Saclay/Notebook/August/8 (→Phusion PCR on pPS16_006 and pPS16_007)
- 09:49, 18 August 2016 (diff | hist) . . (+4) . . Team:Paris Saclay/Notebook/August/17 (→Gel Extraction of 1.2 and Lig 4)
- 09:30, 18 August 2016 (diff | hist) . . (+55) . . Team:Paris Saclay/Notebook/August/17 (→Preparation of the gel for the Gel Extraction of 1.2 and Lig 4)
- 09:25, 18 August 2016 (diff | hist) . . (-9) . . Team:Paris Saclay/Notebook/August/17 (→Q5 PCR on gBlocks (1.2, NM_Sg_RNA, FRB and FKBP) and products of 4.1 and 4.2 gBlocks ligation)
- 08:48, 18 August 2016 (diff | hist) . . (+2) . . Team:Paris Saclay/Notebook/August/17 (→Visualization)
- 08:46, 18 August 2016 (diff | hist) . . (-5) . . Team:Paris Saclay/Notebook/August/16 (→4.1-4.2 ligation)
- 08:46, 18 August 2016 (diff | hist) . . (+13) . . Team:Paris Saclay/Notebook/August/16 (→PCR Clean-up with the NucleoSpin kit)
- 08:39, 18 August 2016 (diff | hist) . . (+8) . . Team:Paris Saclay/Notebook/August/16 (→Q5 PCR on the ligation products and pPS16_008 clones 1 and 2)
- 13:01, 11 August 2016 (diff | hist) . . (+4) . . Team:Paris Saclay/Experiments (→PCR with Phusion High-Fidelity DNA Polymerase from ThermoFisher Scientific)
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