Team:Manchester/Project/Protocols

Manchester iGEM 2016

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Medium and Buffers

20% (w/v) glucose solution


table 1
  1. Prepare the solution by mixing the ingredients stated above.
  2. Sterilize in an autoclave before using it to prepare the SOC medium.

50% (w/v) glycerol solution


table2
  1. Prepare the solution by mixing the ingredients stated above.
  2. Sterilize in an autoclave.

*Source from 2015 iGEM Exeter [link = 2015.igem2015 .org/wiki/images/a/ab/Exeter_Glycerol_recipe.pdf]


Electrophoresis buffers


For nucleic acid DNA/RNA separation

  • TAE buffer (Tris-acetate-EDTA)
  • TBE buffer (Tris-borate-EDTA)
  • LB buffer (Lithium borate)

table3
  1. Prepare the solution by mixing the ingredients stated above.

LB agar (Luria-Bertani agar)


If not using pre-mixed LB agar powder, prepare the materials as below:


table4

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