Team:BGU ISRAEL/Results
Results
One method to assess the activity of our designed mutants is a using the pNP-Butyrate degradation assay. pNP-B is similar in structure to the PET monomer, therefore an increase in its degradation could suggest an increase in the degradation of PET. Upon degradation, pNP-B undergoes a color reaction measurable in a wavelength of 405nm. We performed testing sessions with 96 well plates, testing each variant many times and using the pNP-B, bacteria transformed with an empty vector and without a vector as controls.
In order to test the ability of P. putida to grow in an electrochemical fuel cell we had to assess its ability to product a biofilm on Graphite-felt sheets like those used in the fuel cell.
We incubated the bacteria in a liquid M9 minimal medium with glucose and hanged a sheet of graphite-felt half inside the medium and half outside.
