| BioBrick |
Link |
Part Name |
Description |
Part Type |
| BBa_K2092000 |
http://parts.igem.org/Part:BBa_K2092000 |
AOx without RBS |
This part encodes for the AOx1 protein from Pichia Pastoris. This enzyme oxidises primary alcohols to produce aldehydes and hydrogen peroxide. It is designed with an NDeI restriction site between the prefix and the start of the AOx gene.
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Basic |
| BBa_K2092001 |
http://parts.igem.org/Part:BBa_K2092001 |
alcR without RBS/scar |
This part encodes for AlcR protein, an ethanol-activated transcriptional factor. It binds to alcA promoter and aldA promoter to induce expression of proteins in the presence of ethanol or threonine. |
Basic |
| BBa_K2092002 |
http://parts.igem.org/Part:BBa_K2092002 |
alcA 1P |
This part contains the ethanol/alcR regulated alcA 1 promoter, the native variant of alcA promoter from Aspergillus nidulans which tightly regulates gene expression. It is an improved BioBrick from 2011 iGEM DTU-Denmark BBa_K678001 |
Basic |
| BBa_K2092003 |
http://parts.igem.org/Part:BBa_K2092003 |
alcA 2P |
This part contains the ethanol/alcR regulated alcA 2 promoter, an engineered variant of the alcA promoter from Aspergillus nidulans which tightly regulates gene expression. The lack of one binding site on this promoter is suggested to have 70% decrease in promoter strength in comparison to the native BBa_K2092002. |
Basic |
| BBa_K2092004 |
http://parts.igem.org/Part:BBa_K2092004 or 2005 |
alcR (incl. RBS) |
This part is a twin of BBa_K2092001 with an additional < href="http://parts.igem.org/Part:BBa_J34801" target="_blank">RBS BBa_J34801 being synthesized upstream of the start codon. This part encodes for AlcR protein, an ethanol-activated transcriptional factor. It binds to alcA promoter and aldA promoter to induce expression of proteins in the presence of ethanol or threonine. |
Composite |
| BBa_K2092005 |
http://parts.igem.org/Part:BBa_K2092009 |
alcA2+RBS+amilCP |
This part consists of alcA2 (BBa_K2092003) tagged with a blue chromoprotein, which makes it capable to produce blue colour upon the binding of its ethanol-activated transcription factor AlcR. |
Composite |
| BBa_K2092006 |
http://parts.igem.org/Part:BBa_K2092006 |
CP3+RBS+amilCP |
This part consists of a constitutive promoter (J23110), RBS (B0034) and blue chromoprotein (amilCP). Suitable to be used as a reporter. |
Composite |
| BBa_K2092007 |
http://parts.igem.org/Part:BBa_K2092007 |
CP3+RBS+amilGFP |
This part consists of a constitutive promoter (J23110), RBS (B0034) and yellow chromoprotein (amilGFP). Suitable to be used as a reporter. |
Composite |
| BBa_K2092008 |
http://parts.igem.org/Part:BBa_K2092008 |
CP3+RBS+alcR |
This part consists of a constitutive promoter (J23110), RBS (B0034) and alcR. Suitable to be used to measure the promoter strengths of alcA/aldA. |
Composite |
| BBa_K2092009 |
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