Manchester iGEM 2016
Notebook
Inducible Gene Switch
- Re-suspended DNA (constitutive promoters) from iGEM Distribution kit and transformed them into DH5α strain.
- Growth was found on the negative control plates so re-transformed DNA
- Inoculated of transformed cells.
Pilot Experiment
- Made stocks of our reagents Glucose Stock for 0.005 g/mL, Glucose Oxidase Stock for 0.0025 g/mL, HRP Stock for 250 μL/mL, ABTS for 0.0025 g/mL
Inducible Gene Switch
- Performed miniprep for overnight cultures
- Prepared fresh batch of DH5α chemical competent cells
- Restriction enzyme digest of constitutive promoters and control(pUC19) for validation with EcoRI and PstI
Pilot Experiment
- Still awaiting the arrival of the BMG spectrophotometer
Inducible Gene Switch
- Prepared DH5α chemical competent cells
- Prepared Chloramphenicol antibiotic stock
- Restreaked alcA 1 (BBa_K678001) and spispink(BBa_K1033923 pink chromoprotein)
