We were thrilled after realising that this year’s project from
Lethbridge University was similar to our project. They also wanted to screen a library of nanobodies using a bacterial two-hybrid system. After initiating the contact we had several skype meetings (
see here for a complete skype diary) where we exchanged ideas and talked about each other’s approaches towards the experiments. After a intensive discussion about bacterial two-hybrid systems, we realized that Lethbridge has no positive controls for their two-hybrid system. Therefore we sent them our positive controls:
the HA4-Abl SH2 binding pair including the different mutants of HA4. In this way,we hope to give iGEM Lethbridge the tools to validate their two-hybrid system.
In exchange, Lethbridge provided us with the NS1 peptide from influenza A virus. We wanted to try this peptide as a potential target for our Evobodies. Therefore we could generate Evobodies against the important pathogen influenza A as well.
