Team:FAFU-CHINA/Model/Growth Model
When we made the draft plan about our project, we noticed that NCTU_Formosa 2010’s project made use of Cry11Aa gene. And they constructed the related part BBa_K332011. In their project, they transformed the Cry11Aa gene into E. coli to kill the larvae of mosquitoes. Considering the potential safety problem, using E. coli in the natural environment is not a good idea. Therefore, we decided to use Chlamydomonas reinhardti to express toxin genes such as Cry11Aa. Firstly, we wanted to ask them to send this part to us. And we can transform it to Chlamydomonas reinhardti to test our idea. But we noticed that the codon bias of Chlamydomonas reinhardti was significantly different from the E. Coli. At last, we decided to synthesis the codon-optimized DNA sequence to replace it.
We have mentioned in the wiki that we co-expressed Cry and Cyt by 2A-peptide system. So we also submit the codon-optimized Cry11Aa with 2A-peptide sequence part. Users can link the other genes by infusion technology.
Therefore, we submitted the part which contained the Chlamydomonas reinhardti codon-optimized DNA sequence as a part of our improvement project. Meanwhile, we submit the codon-optimized Cry11Aa with 2A-peptide sequence part.
