Result and Discussion
Confirmation antibacterial activity of farnesol.
First, we examined our working hypothesis to “Flavorator” that farnesol can show either the antibacterial or bacteriostatic activity in a box like “KOZOKO”. The results clearly showed that farnesol had antibacterial properties. In the literatures, farnesol have antibacterial volatiles. Farnesol is produced after complicated pathways, so for their syntheses, various enzymes are required. In E. coli, farnesol may be synthesized. In this context, we designed our system for establishing the concept of “Flavorator” to build up a brand-new biosynthetic pathways, in which farnesol is produced in the E. coli. In doing so, we transfered the three types of genes listed below to create the hyper-producer E. coli of farnesol. We examined our working hypothesis to “Flavorator” that farnesol can show either the antibacterial or bacteriostatic activity in a box like Kozoko.