Team:Oxford/Notebook
Notebook
Introduction
This page documents all the experiments we carried out in the wet lab as a part of our project. The details of the process we carried out can be found on our protocols page, and the chemicals we used can be found on the chemicals page. The interlab project was carried out alongside the rest of the wet lab but we have recorded it separately.
Week 1
Day 1
Day 2
Day 3
Day 4
Day 5
Week 2
Day 1
Day 2
Day 3
Day 4
Day 5
Week 3
Day 1
Day 2
Day 3
Day 4
Day 5
Week 4
Day 1
Day 2
Day 3
Day 4
Day 5
Week 5
Day 1
Day 2
Day 3
Day 4
Day 5
Week 6
Day 1
Day 2
Day 3
Day 4
Day 5
Week 7
Day 1
Day 2
Day 3
Day 4
Day 5
Week 8
Day 1
Day 2
Day 3
Day 4
Day 5
Week 9
Day 1
Day 2
Day 3
Day 4
Day 5
Week 10
Day 1
Day 2
Day 3
Day 4
Day 5
Week 11
Day 1
Day 2
Day 3
Day 4
Day 5
Week 12
Day 1
Day 2
Day 3
Day 4
Day 5
Week 13
Day 1
Day 2
Day 3
Day 4
Day 5
Week 14
Day 1
Day 2
Day 3
Day 4
Day 5
Week 15
Day 1
Day 2
Day 3
Day 4
Day 5
Week 16
Day 1
Day 2
Day 3
Day 4
Day 5
Interlab
Day 1
Transformed the 5 interlab parts into DH5a as per the transformation protocol.
Day 2
Transformations mostly unsuccessful. Started making new competent cells.
Day 3
Second day of competent cell preparation, cells frozen and put in the freezer.
Day 4
LB media preparation and re-transformation of the interlab parts.
Day 5
Had colonies from all interlab parts except PC. Picked colonies from all successful plates. Retransformed PC.
Day 6
PC didn't transform again. After measuring the sample we were sent with nanodrop we found the sample has no DNA in it. Requested a second sample from iGEM HQ Nevertheless we did a third transformation for PC. NC and TD1-3 were miniprepped and sent for sequencing.
Day 7
TD1 sequencing successful, others had the wrong part in so retransformed these. PC colonies successful despite nanodrop so picked colonies for these.
Day 8
PC miniprepped, digested and sent for sequencing. Colonies picked for re-transformed TD2, TD3 and NC.
Day 9
PC sequencing unsuccessful. TD2, TD3 and NC miniprepped and sent for sequencing.
Day 10
TD2 sequencing successful, others not. PC, NC and TD3 transformed again
Day 11
PC, NC and TD3 colonies picked.
Day 12
PC, NC and TD3 miniprepped and sent for sequencing.
Day 13
NC and TD3 sequencing successful but PC wrong so retransformed again.
Day 14
Picked colonies for PC.
Day 15
PC was miniprepped and digested. Gel showed it contained the wrong part so we transformed the part from the distribution kit instead.
Day 16
Colonies picked for PC.
Day 17
PC miniprepped, digested and sent for sequencing. Produced more competent cells.
Day 18
PC sequencing correct! All 5 interlab parts were transformed into MG1655
Day 19
Made the calibration curves for the OD600 and the FITC standard curve Colonies picked for all the interlab parts.
Day 20
Tested iGEM's protocol for the OD600 and the fluorescence. Picked more colonies for all 5 parts.
Day 21
Repeated iGEM's protocol and started Oxford iGEM's protocol.
Day 22
Finished Oxford iGEM's protocol and submitted the data obtained using iGEM's method to iGEM
