Team:Missouri Rolla/Safety

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All S&T iGEM members must be trained through our online and in-lab Lab Training Program before contributing to the project in lab. Our Lab Training Manual, which contains a variety of safety and procedure information, and our online lessons are currently being revised to better prepare future lab workers. The Lab Training Program culminates in the "mini-project," where trainees are given a small task and must produce a final product using parts from the iGEM DNA Distribution. Our goal is to train safe and productive lab workers by giving them a chance to try each procedure themselves under the supervision of an experienced member.

We used E. coli DH5α, a non-pathogenic strain, for all assembly and as the chassis for testing. We attempted to express the mevalonate pathway to produce (E)-beta-ocimene, a safe terpene with pleasant scent. We also used Streptomyces roseus, another non-pathogenic strain. The genome of S. roseus contains two genes of interest, leupA and leupB, which are potentially involved in the production of leupeptin, a protease inhibitor. Our final safety form may be found