Team:Tokyo Tech/Toxin Assay/Overview

• 
• 
• 
  • 
    • 
    • 
    • 
    • 
  • 
    • 
    • 
    • 
  • 
  • 
    • 
    • 
• 
• 
  • 
  • 
  • 
  • 
• 
  • 
  • 
  • 
• 
• 
  • 
  • 
  • 
  • 
  • 
  • 

3-1-0. Overview

Contents

3-1-0. TA system

3-1-1.　Adjustment of MazF expression

3-1-2. mazEF System Assay

   3-1-2-1. Stop & Go

   3-1-2-2. Go & Stop

3-1-3. Control of Cell Growth

3-1-4. Reference

3-1-0. TA system

The experiments of iGEM 2016 team Tokyo_Tech are divided into Toxin, AHL, AmiE, and the temperature-dependent promoter Assay. This section describes Toxin Assay. TA system is a function that controls cell growth on the genomic DNA of many microorganisms.Preceding iGEM teams studied on the following TA systems; ccdB-ccdA (iGEM 2014 team ULB-Brussels and iGEM 2013 team UGent), Kid-Kis, Zeta-Epsilon(iGEM 2014 team Wageningen UR) and Hok-Soc(iGEM 2015 team UMaryland). Almost all the iGEM teams dealing with TA system aimed to use it as Kill Switch or substitution for antibiotics. On the other hand, we aim to study the interaction of toxin and antitoxin in a cell. Besides some teams failed to show enough data of TA system assay. Although UCSF 2012 experienced the communication through TA system including mazEF system, they did not introduce toxin and antitoxin into a cell and could not show mazEF system assay data. Our results show that the cell growth can be controlled by mazEF system. In iGEM 2016 team Tokyo_Tech, we have studied on mazEF system, which has been studied well as the TA system.