Team:BGU ISRAEL/Integrated Practices
Human Practice
Human practice is an integral and inseparable part of iGEM. We, PlastiCure, chose to focus on a major environmental subject that concern the world. Therefore, one of our goals were to reach a diverse community, expose and explain about the crucial problem, the plastic hazard, humanity face with. We believe that in order to maintains a difference and create an environmental impact, society need to be aware to the issue. In our project we highlight this filed thought different strategic - first, we acquired extensive knowledge about plastic waste then, we focused on passing the knowledge to the general public through public engagement and various media channels. You can read more about every one of them in the specific label.
Plastic Art
Policies
Acquiring Knowledge
Every science research first requires a collect of information and introducing of the field. One of our major goals in the project, was to raise awareness of the plastic hazard. Thus, before we could pass our knowledge to others, we did a comprehensive investigation of plastic's implications. We met senior professors and researchers who focuses on different aspects of plastic waste.
Prop. Alex Sivan, a researcher from our university that one of his research interests is Microbial degradation of plastic wastes. We consulted prop. Sivan about the enzyme we should work with. Along to wide Literature research, we have learned that LC - Cutinase is an enzyme who knows for his ability to break PET first bond in the metabolic degradation pathway. We noticed that there is an extend research about this enzyme property. From that point we decided to work with LC - Cutinase as the PET first bond breaker. Dr. Sarel Flaysman - Principal investigator from Department of Biological Chemistry, Weizmann Institute of Science. We contact Dr. Flaysman in order to introduce him our project. Dr. Flaysman suggest us to use his algorithm- PROSS, who preform rational mutations in proteins and improve their activity by making them more thermodynamic stable.
After we collect that info, we were ready to start the lab research. For that we met Prof. Amir Aharoni - a researcher from our university that focus in Protein Engineering for the study of complex biological processes. Prof. Aharoni helped us to apply our theoretical knowledge to lab terms. Meanwhile we also investigated the metabolic pathway part, we read lot of article and found out about the Pseudomonas putida bacterium.
Prop. De Lorenzo - his research exploits advanced molecular biology and genetic engineering of microorganisms for the sake of biomonitoring, bioremediation etc. He use in particular P.Putida. We contact him and ask about the P.Putida bacterium.
Prop. De Lorenzo answered our questions, sent us pSEVA plasmids and turned us to Prof. Dan Tawfik and Dr. Halim Jubran, Department of Bimolecular Sciences, Weizmann Institute of Science, That provided us with the P.putida KT 2440. We also consulate Dr. Shimon Bershtein, Faculty of Natural Sciences, Ben-Gurion University of the Negev, about Assistance with P.putida resistances and genetic engineering. As we gather information about our Substrate we moved to design our science research. Thus we address our PIs for science advises and met diverse researchers who helped us combine an efficient work patterns.
All project long we continued to encounter experts who deal with plastic as a raw material in industrial ways, the main ones was - Coca-Cola Company, Genome compiler and Carasso Science Park . We have learned about plastic's convenient properties that makes him so popular and easy to use, such as- flexibility, rigidity, lucidity and much more.
Finally, we aimed to get familiar with plastic waste degradation solutions. Therefore, we visit 'Neot Hovav- eco industrial park', 'Aviv- Recycling Industries' and 'ADAMA Agricultural Solutions' . At our visit in Aviv recycling plant we saw and understood the process of plastic recycling – from the arrival of the plastic bottle to the plant, to transforming it to raw material for other products.
One of the most interesting and important things we've learned during the tour is that 99% of the raw material (the output of the recycle process) is than used to producing products that cannot go through the recycle process again. It was here, that we really understood the enormous damage lies in plastic waste and that we have to find a different solution to deal with this problem.
The investigate process we have done helped us understand how major and severe the issue of accumulate of plastic waste is. This increase our motivation and drove us to work harder in order to find an efficient solution.
Public Outreach
After we extend our knowledge we turn to pass it to the public. We tried to reach as many people as possible, in order to raise their awareness to plastics solution disadvantages thus encourage controlled consumption. We did it thought public events and media channels. First we opened a twitter, Instagram and facebook accounts, followed by thousands of people. We updated those accounts daily with new data about the wide world plastic waste problem, our project, and many fact related to the subject. In the social media we also published our events, answer people questions and attract people attention towards our project.
Another way to Engage and educate the crowd was by publish in many newspapers all over Israel, among them 'Haaretz', the oldest and one of the biggest newspaper in Israel. We were published it two issues of 'Haaretz'- the local Israeli issue and the English newsletter version.
In the article we interviewed about our environmental concern and the research we conduct to solve it. We also mentioned the advance synthetic biology tools we are using in the lab and talked about the significant attribute of iGEM project to science. For the full article click here
One of our best accomplishments to achieved was Queen Noor twittered 'HAARETZ' article about our project- this tweet reacted by almost 250 people and reached to all thousands of Queen Noor's followers.
we have reached was winning 'Green Campus award'. 'Green Campus' is a green competition occur in our university. Any student with an Innovative idea concern the environment can apply. We won first place and got recognition from our university.
In order to reach diverse communities, we also participated in an environment radio show, hosted by south radio channel. On the show we discussed the existed plastic waste solutions and explained the necessary of biosynthetic solution.
To make our project more accessible we created a fun computer game and animation video. Those attracted and exposed many people to our goals. The computer game demonstrate our idea as a simple task, which a bacteria eating plastic parts by shooting them. Because the mission is not that easy, we made the game in a medium-high level of difficulty. If you lose the game, a picture of ocean full of plastic waste is shown. We sent the game to all iGEM groups along to our project description and ask them to spread it as much as they can. We also upload the game to our website and our social media accounts. You can check it out here
The animation video we created present our project in attractive and simple way. Due to that people who don't have synthetic biology background can understand the project and the science behind it.
Public Engagement
This was a really, really big year for me. I got to go home for Thanksgiving and sit at the adults' table. That's 'cause, you know, somebody had to die for me to move up a plate.
"osim rehov / making a street" event:
"Lyla lavan / white night" event:
"Science with a beer" event:
Address:
Ben-Gurion University of the Negev
Ben Gurion 1, Beer Sheva 8410501, Israel
Mail: igembgu2016@gmail.com
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