Template:CAPS Kansas test
CAPS Kansas
Manipulating Omp pores & AcrAB-TolC efflux pumps using CRISPR/dCas9 to enhance E. coli antibiotic susceptibility.
Our Project
Antibiotics are a hallmark of modern medicine having saved numerous lives since the discovery of penicillin in 1928. Bacteria resistant to antibiotics emerged shortly after their initial use, and is an increasing problem as pathogenic strains of bacteria evolve resistance to multiple drugs. In an effort to increase antibiotic susceptibility, we will use CRISPR/Cas9 technologies to disrupt intrinsic resistance mechanisms in E. coli. Specifically, we plan to enhance the expression of Omp pores while also inhibiting the functioning of AcrAB-TolC efflux pumps. Mechanism for effective delivery of a functioning CRISPR system in clinical settings will be explored.
Our Team
Ipsum consequat
Donec imperdiet consequat consequat. Suspendisse feugiat congue
posuere. Nulla massa urna, fermentum eget quam aliquet.
Nam elementum nisl et mi a commodo porttitor. Morbi sit amet nisl eu arcu faucibus hendrerit vel a risus. Nam a orci mi, elementum ac arcu sit amet, fermentum pellentesque et purus. Integer maximus varius lorem, sed convallis diam accumsan sed. Etiam porttitor placerat sapien, sed eleifend a enim pulvinar faucibus semper quis ut arcu. Ut non nisl a mollis est efficitur vestibulum. Integer eget purus nec nulla mattis et accumsan ut magna libero. Morbi auctor iaculis porttitor. Sed ut magna ac risus et hendrerit scelerisque. Praesent eleifend lacus in lectus aliquam porta. Cras eu ornare dui curabitur lacinia.
Safety
While CAPS Kansas has certainly dedicated itself to the frontier of microbiology research and exploration, safety is still the top priority. CAPS Kansas has followed proper safety guidelines for all labs and experiments. Non-pathogenic strains of Escherichia coli were the subjects of all experiments. All tools were properly sanitized and disposed of after use. Hazardous chemicals were handled with full diligence and care under the watchful supervision of lab experts Mr. Kessler and Mr. Whalen. CAPS Kansas takes safety seriously. We encourage all iGEM teams to follow proper safety guidelines, and to never under any circumstances forsake human safety for fast results.
Human Practices
CAPS Kansas believes that proper, engaging STEM education begins much earlier than high school. By combing pop culture with fun science, CAPS Kansas hopes to inspire and cultivate young scientists.
Attributions
Genetic enginenering is a challenging process. Thankfully, CAPS Kansas has had the pleasure of working with many talented and hard-working individuals.
Awards
Donec imperdiet consequat consequat. Suspendisse feugiat congue
posuere. Nulla massa urna, fermentum eget quam aliquet.
