Wednesday, September 28th
Tasks:
Jordan
- Colony PCR all pET28a previously picked colonies
- Transform SS-Cas9 in tet plasmid into JC8031
Sam
- Autoclave 5 beveled Erlenmeyers LB for 25 mL cultures, also all of the tips
- Rerun the PCR from yesterday that Paul did (SS-cas9 with res digest overhangs)
- Make extra plates of the TorA and YcdO colonies 1 and 2
Jordan
- Colony PCR all pET28a previously picked colonies
- Transform SS-Cas9 in tet plasmid into JC8031
Sam
- Autoclave 5 beveled Erlenmeyers LB for 25 mL cultures, also all of the tips
- Rerun the PCR from yesterday that Paul did (SS-cas9 with res digest overhangs)
- Make extra plates of the TorA and YcdO colonies 1 and 2
