Team:Tokyo Tech/Toxin Assay/Queens capricious

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3-1-4 YafO System Assay

Contents

1. Introduction

2. Summary of the Experiment

   2-1. Confirming YafO Function as Toxin on Agar plates

   2-2. Toxin-Antitoxin Assay

3. Results

   3-1. Confirming YafO Function as Toxin on Agar plates

   3-2. Toxin-Antitoxin Assay

4. Discussion

5. Materials and Methods

   5-1. Strain

   5-2. Plasmid

     5-2-1. Assay to Confirm YafO function as toxin on agar plates

     5-2-2. toxin-antitoxin assay

   5-3. Assay Protocol

     5-3-1. Confirming YafO function as toxin on agar plate

     5-3-2. toxin-antitoxin assay

6. Reference

1. Introduction