Team:Tokyo Tech/Toxin Assay/Queens capricious

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3-1-4 YafO System Assay

Contents

1. Introduction

2. Summary of the Experiment

   2-1. Confirming YafO Function as Toxin on Agar plates

   2-2. Toxin-Antitoxin Assay

3. Results

   3-1. Confirming YafO Function as Toxin on Agar plates

   3-2. Toxin-Antitoxin Assay

4. Discussion

5. Materials and Methods

   5-1. Strain

   5-2. Plasmid

     5-2-1. Assay to Confirm YafO function as toxin on agar plates

     5-2-2. toxin-antitoxin assay

   5-3. Assay Protocol

     5-3-1. Confirming YafO function as toxin on agar plate

     5-3-2. toxin-antitoxin assay

6. Reference

1. Introduction

　Our project, the story of “Snow White” is constructed based on mazEF system, which is one of toxin-antitoxin (TA) system on E. coli genomic DNA. At the same time, we are interested in other TA systems and we carried out assay using yafNO system.

2. Summary of the Experiment