CAPS Kansas
Manipulating Omp pores & AcrAB-TolC efflux pumps using CRISPR/dCas9 to enhance E. coli antibiotic susceptibility.
Our Project
Antibiotics are a hallmark of modern medicine having saved numerous lives since the discovery of penicillin in 1928. Bacteria resistant to antibiotics emerged shortly after their initial use, and is an increasing problem as pathogenic strains of bacteria evolve resistance to multiple drugs. In an effort to increase antibiotic susceptibility, we will use CRISPR/Cas9 technologies to disrupt intrinsic resistance mechanisms in E. coli. Specifically, we plan to enhance the expression of Omp pores while also inhibiting the functioning of AcrAB-TolC efflux pumps. Mechanism for effective delivery of a functioning CRISPR system in clinical settings will be explored.
Our Team
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Safety
While CAPS Kansas has certainly dedicated itself to the frontier of microbiology research and exploration, safety is still the top priority. CAPS Kansas has followed proper safety guidelines for all labs and experiments. Non-pathogenic strains of Escherichia coli were the subjects of all experiments. All tools were properly sanitized and disposed of after use. Hazardous chemicals were handled with full diligence and care under the watchful supervision of lab experts Mr. Kessler and Mr. Whalen. CAPS Kansas takes safety seriously. We encourage all iGEM teams to follow proper safety guidelines, and to never under any circumstances forsake human safety for fast results.
Human Practices
CAPS Kansas believes that proper, engaging STEM education begins much earlier than high school. By combing pop culture with fun science, CAPS Kansas hopes to inspire and cultivate young scientists.
Attributions
Genetic enginenering is a challenging process. Thankfully, CAPS Kansas has had the pleasure of working with many talented and hard-working individuals.
Awards
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