Team:USP UNIFESP-Brazil/Parts
AlgAranha Team USP_UNIFESP-Brazil
Part Table
The protein structure of spider silk is composed of two constant terminal domains and a variable middle structural domain. Our design uses the substitution of this middle domain by a protein of interest, as used by Team:UCLA in 2014. In our case, we are using enzybiotics.
Our major contribution of a Basic Part for the iGEM community is a BioBrick coding for the catalytic protein domain of lysostaphin (BBa_K2136002) optimized for Chlamydomonas reinhardtii genome. This is an antibiotic peptide domain which can be used by future teams in the development of projects fighting antibiotic resistant Staphylococcus or working with its population dynamics.
Our favourite composite part was the 5' cassete for Chlamydomonas transgenic expression(BBa_K2136010). We validated it through a series of measurements which show the production and secretion of mCherry protein by Chlamydomonas. A detailed descriptions is available in our Proof of concept page.
