Team:Nagahama/Protocol

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Medium

LB medium (100 mL liquid) 1.Measure 1g Tripton 2.Measure 0.5g Yeast Extract 3.Measure 1g Nacl 4.Add 100mL H2O 5.autoclave(121℃ 20min)

2×YT medium (100mL liquid) 1.Measure 1.6g Tripton 2.Measure 1g Yeast Extract 3.Measure 0.5g Nacl 4.Add 100mL H2O 5.autoclave(121℃ 20min)