Team:CGU Taiwan/Safety

Leijuvant

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Safety

(1)Short introduction of our project

Transgenic Leishmania that can be inactivated by light exposure acts as a safe carrier to deliver specific antigens to the APCs for T cells and humoral response. Based on this concept, we established a new model system to generate antigen-specific inactive Leishmania adjuvant (Leijuvant).

(2)What organisms do we use?

Leishmania, DC2.4, E.coli(DH5α, BL21), C57BL/6 mice

(3)Which parts do we use?

Full length of HA and OVA: We used full length sequence of hemagglutinin (HA) of H1N1 and Ovalbumin as our testing antigen that is expressed in Leishmania.

5’UTR, 3’UTR, 2.3kb intron: the sequence is originally from Leishmania genome. Act as promotor, terminator, and expression modulating part in our device.