We use DH-5α for DNA cloning and we also use the E.coli as a model microorganisms to check the function of cloned DNA. We were going to transform A. xylinum. A. xylinum was used in an industry field and is normally present in the environment.
Q2. What risks does our project pose at the laboratory stage? What actions are we takinｇ to reduce those risks?
A2. ・We wear a lab coat and gloves when we use microorganisms.
・We have sterilized our used materials by autoclave, ethanol.
・We have experimented in clean bench to prevent our organism from escaping from lab.
・We sterilized all waste before we throw away.
・We have a P2 level laboratory and we use the room.
