8/17
restriction emzyme fragmation
K592024 B0015 C0051→Eco RI & SpeI
I14144 R100 B0015 →XbaI & PstI
EcoRI XbaI PstI →Promega
SpeI→Takara
8/18
transcription
R0051 K592024 I14044 B0015 R0010
C0051(psbic3)→E・coli-DH5X
8/18
miniprep
J04450
colony PCR
K592024 I14044 B0015 R0010 C0051
8/23
restriction emzyme fragmation
9/16
miniprep
restriction emzyme fragmation
9/19
miniprep
9/21
miniprep
9/22
restriction emzyme fragmation
miniprep
purify a gel
9/23
miniprep
purify a gel
9/24
restriction emzyme fragmation
9/25
purify a gel
ligation
transformation
9/26
miniprep
9/27
purify a gel
restriction emzyme fragmation
9/28
restriction emzyme fragmation
9/30
restriction emzyme fragmation
purify a gel
10/1
ligation
transformation
pre-culture
purification of antisensence
10/2
pre-culture
miniprep
electrophoresis to confirm
10/3
miniprep
PCR
pre-culture
purify a gel
10/4
restriction emzyme fragmation
purify a gel
miniprep
electrophoresis to confirm
10/5
ligation
transformation
purify a gel
restriction emzyme fragmation
10/6
KIB…miniprep
electrophoresis to confirm
purify a gel
10/7
restriction emzyme fragmation
ligation
transformation
10/8
miniprep
purify a gel
restriction emzyme fragmation
10/9
pre-culture
purify a gel
10/10
ligation
transformation
10/11
pre-culture
miniprep
10/12
purify a gel
10/13
electrophoresis to confirm
10/14
restriction emzyme fragmation
10/15
electrophoresis to confirm
restriction emzyme fragmation
10/16
electrophoresis to confirm