Labonote
- 8/17
- restriction emzyme fragmation
K592024 B0015 C0051→Eco RI & SpeI
I14144 R100 B0015 →XbaI & PstI
EcoRI XbaI PstI →Promega
SpeI→Takara
- 8/18
transcription
R0051 K592024 I14044 B0015 R0010
C0051(psbic3)→E・coli-DH5X
- 8/18
miniprep
J04450
colony PCR
K592024 I14044 B0015 R0010 C0051
- 8/23
restriction emzyme fragmation
- 8/24
3A assembly
Ligation
PCR
R0051 K592024 I14044 B0015 R0010 C0051
- 8/25
Miniprep
J04450
8/26
Restriction enzyme treatment
- 8/29
Purity a gel
- 8/30
PCR
- 8/31
PCR
3A assembly
K595024 + I14044
- 9/1
PCR
K595024 I14044 C0051 R0010 B0015
purity a gel
preculture
K595024 I14044 C0051 R0010 B0015
9/2
Miniprep
K592024 I14044 C0051 R0010 B0015
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
- 9/3
Miniprep
K592024 I14044 C0051 R010 B0015 J04450
- 9/5
Restriction enzyme treatment
- 9/6
PCR
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
- 9/7 Miniprep
K592024 I14044 C0051 R010 B0015 J04450
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
- 9/8
Miniprep
K592024 I14044 C0051 R010 B0015 J04450
- 9/8
PCR
- 9/12
Gel purification
- 9/13
Restriction enzyme treatment
C0051 R010 B0015X B0015E J04450
Pre-culture
K592024 I14044 J04450
Restriction enzyme treatment
- 9/14
Restriction enzyme treatment
Miniprep
K592024 I14044 J04450
- /15
Transformation
Restriction enzyme treatment
K592024 I14044 J04450
- 9/16
PCR
K592024 I14044 J04450
Miniprep
Restriction emzyme fragmation
- 9/19
Miniprep
- 9/21
Miniprep
- 9/22
Restriction emzyme fragmation
Miniprep
Purify a gel
- 9/23
Miniprep
Purify a gel
- 9/24
restriction emzyme fragmation
- 9/25
purify a gel
ligation
transformation
- 9/26
Miniprep
- 9/27
Purify a gel
Restriction emzyme fragmation
9/28
Restriction emzyme fragmation
- 9/30
Restriction emzyme fragmation
Purify a gel
- 10/1
Ligation
transformation
Pre-culture
Purification of antisensence
- 10/2
Pre-culture
Miniprep
Electrophoresis to confirm
- 10/3
miniprep
PCR
Pre-culture
Purify a gel
- 10/4
Restriction emzyme fragmation
Purify a gel
Miniprep
Electrophoresis to confirm
- 10/5
Ligation
Transformation
Purify a gel
Restriction emzyme fragmation
- 10/6
KIB…miniprep
Electrophoresis to confirm
Purify a gel
- 10/7
Restriction emzyme fragmation
Ligation
Transformation
- 10/8
Miniprep
Purify a gel
Restriction emzyme fragmation
- 10/9
Pre-culture
Purify a gel
- 0/10
Ligation
Transformation
- 10/11
pre-culture
Miniprep
- 10/12
Purify a gel
- 10/13
Electrophoresis to confirm
- 10/14
Restriction emzyme fragmation
- 10/15
Electrophoresis to confirm
Restriction emzyme fragmation
- 10/16
Electrophoresis to confirm
- 8/18