Labonote
- 8/17
- restriction emzyme fragmation
K592024 B0015 C0051→Eco RI & SpeI
I14144 R100 B0015 →XbaI & PstI
EcoRI XbaI PstI →Promega
SpeI→Takara
- 8/18
- transcription
R0051 K592024 I14044 B0015 R0010
C0051(psbic3)→E・coli-DH5X
- 8/18
- miniprep
J04450
colony PCR
K592024 I14044 B0015 R0010 C0051
- 8/23
- restriction emzyme fragmation
- 8/24
- 3A assembly
Ligation
PCR
R0051 K592024 I14044 B0015 R0010 C0051
- 8/25
- Miniprep
J04450
- 8/26
- Restriction enzyme treatment
- 8/29
- Purity a gel
- 8/30
- PCR
- 8/31
- PCR
3A assembly
K595024 + I14044
- 9/1
- PCR
K595024 I14044 C0051 R0010 B0015
purity a gel
preculture
K595024 I14044 C0051 R0010 B0015
- 9/2
- Miniprep
K592024 I14044 C0051 R0010 B0015
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
- 9/3
- Miniprep
K592024 I14044 C0051 R010 B0015 J04450
- 9/5
- Restriction enzyme treatment
- 9/6
- PCR
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
- 9/7
- Miniprep
K592024 I14044 C0051 R010 B0015 J04450
Restriction enzyme treatment
Pre-culture
K592024 I14044 C0051 R010 B0015 J04450
- 9/8
- Miniprep
K592024 I14044 C0051 R010 B0015 J04450
- 9/8
- PCR
- 9/12
- Gel purification
- 9/13
- Restriction enzyme treatment
C0051 R010 B0015X B0015E J04450
Pre-culture
K592024 I14044 J04450
Restriction enzyme treatment
- 9/14
- Restriction enzyme treatment
Miniprep
K592024 I14044 J04450
- 9/15
- Transformation
Restriction enzyme treatment
K592024 I14044 J04450
- 9/16
- PCR
K592024 I14044 J04450
Miniprep
Restriction emzyme fragmation
- 9/19
- Miniprep
- 9/21
- Miniprep
- 9/22
- Restriction emzyme fragmation
Miniprep
Purify a gel
- 9/23
- Miniprep
Purify a gel
- 9/24
- Restriction emzyme fragmation
- 9/25
- Purify a gel
Ligation
Transformation
- 9/26
- Miniprep
- 9/27
- Purify a gel
Restriction emzyme fragmation
- 9/28
- Restriction emzyme fragmation
- 9/30
- Restriction emzyme fragmation
Purify a gel
- 10/1
- Ligation
transformation
Pre-culture
Purification of antisensence
- 10/2
- Pre-culture
Miniprep
Electrophoresis to confirm
- 10/3
- miniprep
PCR
Pre-culture
Purify a gel
- 10/4
- Restriction emzyme fragmation
Purify a gel
Miniprep
Electrophoresis to confirm
- 10/5
- Ligation
Transformation
Purify a gel
Restriction emzyme fragmation
- 10/6
- KIB…miniprep
Electrophoresis to confirm
Purify a gel
- 10/7
- Restriction emzyme fragmation
Ligation
Transformation
- 10/8
- Miniprep
Purify a gel
Restriction emzyme fragmation
- 10/9
- Pre-culture
Purify a gel
- 10/10
- Ligation
Transformation
- 10/11
- pre-culture
Miniprep
- 10/12
- Purify a gel
- 10/13
- Electrophoresis to confirm
- 10/14
- Restriction emzyme fragmation
- 10/15
- Electrophoresis to confirm
Restriction emzyme fragmation
- 10/16
- Electrophoresis to confirm